Differential gene and protein expression between rat tibial nerve and common peroneal nerve during Wallerian degeneration
Wallerian degeneration and nerve regeneration after injury are complex processes involving many genes, proteins and cytokines. After different peripheral nerve injuries the regeneration rate can differ. Whether this is caused by differential expression of genes and proteins during Wallerian degenera...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Neural Regeneration Research |
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| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2019;volume=14;issue=12;spage=2183;epage=2191;aulast=Lin |
| _version_ | 1818269679373778944 |
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| author | Yao-Fa Lin Zheng Xie Jun Zhou Gang Yin Hao-Dong Lin |
| author_facet | Yao-Fa Lin Zheng Xie Jun Zhou Gang Yin Hao-Dong Lin |
| author_sort | Yao-Fa Lin |
| collection | DOAJ |
| description | Wallerian degeneration and nerve regeneration after injury are complex processes involving many genes, proteins and cytokines. After different peripheral nerve injuries the regeneration rate can differ. Whether this is caused by differential expression of genes and proteins during Wallerian degeneration remains unclear. The right tibial nerve and the common peroneal nerve of the same rat were exposed and completely cut through and then sutured in the same horizontal plane. On days 1, 7, 14, and 21 after surgery, 1–2 cm of nerve tissue distal to the suture site was dissected out from the tibial and common peroneal nerves. The differences in gene and protein expression during Wallerian degeneration of the injured nerves were then studied by RNA sequencing and proteomic techniques. In the tibial and common peroneal nerves, there were 1718, 1374, 1187, and 2195 differentially expressed genes, and 477, 447, 619, and 495 differentially expressed proteins on days 1, 7, 14, and 21 after surgery, respectively. Forty-seven pathways were activated during Wallerian degeneration. Three genes showing significant differential expression by RNA sequencing (Hoxd4, Lpcat4 and Tbx1) were assayed by real-time quantitative polymerase chain reaction. RNA sequencing and real-time quantitative polymerase chain reaction results were consistent. Our findings showed that expression of genes and proteins in injured tibial and the common peroneal nerves were significantly different during Wallerian degeneration at different time points. This suggests that the biological processes during Wallerian degeneration are different in different peripheral nerves after injury. The procedure was approved by the Animal Experimental Ethics Committee of the Second Military Medical University, China (approval No. CZ20160218) on February 18, 2016. |
| first_indexed | 2024-12-12T20:58:13Z |
| format | Article |
| id | doaj.art-93ffd01fa41842a49a979b68d369ef1b |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-12T20:58:13Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-93ffd01fa41842a49a979b68d369ef1b2022-12-22T00:12:15ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742019-01-0114122183219110.4103/1673-5374.262602Differential gene and protein expression between rat tibial nerve and common peroneal nerve during Wallerian degenerationYao-Fa LinZheng XieJun ZhouGang YinHao-Dong LinWallerian degeneration and nerve regeneration after injury are complex processes involving many genes, proteins and cytokines. After different peripheral nerve injuries the regeneration rate can differ. Whether this is caused by differential expression of genes and proteins during Wallerian degeneration remains unclear. The right tibial nerve and the common peroneal nerve of the same rat were exposed and completely cut through and then sutured in the same horizontal plane. On days 1, 7, 14, and 21 after surgery, 1–2 cm of nerve tissue distal to the suture site was dissected out from the tibial and common peroneal nerves. The differences in gene and protein expression during Wallerian degeneration of the injured nerves were then studied by RNA sequencing and proteomic techniques. In the tibial and common peroneal nerves, there were 1718, 1374, 1187, and 2195 differentially expressed genes, and 477, 447, 619, and 495 differentially expressed proteins on days 1, 7, 14, and 21 after surgery, respectively. Forty-seven pathways were activated during Wallerian degeneration. Three genes showing significant differential expression by RNA sequencing (Hoxd4, Lpcat4 and Tbx1) were assayed by real-time quantitative polymerase chain reaction. RNA sequencing and real-time quantitative polymerase chain reaction results were consistent. Our findings showed that expression of genes and proteins in injured tibial and the common peroneal nerves were significantly different during Wallerian degeneration at different time points. This suggests that the biological processes during Wallerian degeneration are different in different peripheral nerves after injury. The procedure was approved by the Animal Experimental Ethics Committee of the Second Military Medical University, China (approval No. CZ20160218) on February 18, 2016.http://www.nrronline.org/article.asp?issn=1673-5374;year=2019;volume=14;issue=12;spage=2183;epage=2191;aulast=Linnerve regeneration; peripheral nerves; peripheral nerve injuries; Wallerian degeneration; tibial nerve; common peroneal nerve; RNA sequencing; proteomic; real-time quantitative polymerase chain reaction; neural regeneration |
| spellingShingle | Yao-Fa Lin Zheng Xie Jun Zhou Gang Yin Hao-Dong Lin Differential gene and protein expression between rat tibial nerve and common peroneal nerve during Wallerian degeneration Neural Regeneration Research nerve regeneration; peripheral nerves; peripheral nerve injuries; Wallerian degeneration; tibial nerve; common peroneal nerve; RNA sequencing; proteomic; real-time quantitative polymerase chain reaction; neural regeneration |
| title | Differential gene and protein expression between rat tibial nerve and common peroneal nerve during Wallerian degeneration |
| title_full | Differential gene and protein expression between rat tibial nerve and common peroneal nerve during Wallerian degeneration |
| title_fullStr | Differential gene and protein expression between rat tibial nerve and common peroneal nerve during Wallerian degeneration |
| title_full_unstemmed | Differential gene and protein expression between rat tibial nerve and common peroneal nerve during Wallerian degeneration |
| title_short | Differential gene and protein expression between rat tibial nerve and common peroneal nerve during Wallerian degeneration |
| title_sort | differential gene and protein expression between rat tibial nerve and common peroneal nerve during wallerian degeneration |
| topic | nerve regeneration; peripheral nerves; peripheral nerve injuries; Wallerian degeneration; tibial nerve; common peroneal nerve; RNA sequencing; proteomic; real-time quantitative polymerase chain reaction; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2019;volume=14;issue=12;spage=2183;epage=2191;aulast=Lin |
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