“Beige” Cross Talk Between the Immune System and Metabolism
With thymic senescence the epithelial network shrinks to be replaced by adipose tissue. Transcription factor TBX-1 controls thymus organogenesis, however, the same TBX-1 has also been reported to orchestrate beige adipose tissue development. Given these different roles of TBX-1, we have assessed if...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-06-01
|
| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fendo.2019.00369/full |
| _version_ | 1818178515774734336 |
|---|---|
| author | Krisztina Banfai Krisztina Banfai David Ernszt David Ernszt Attila Pap Peter Bai Peter Bai Peter Bai Peter Bai Kitti Garai Kitti Garai Djeda Belharazem Judit E. Pongracz Judit E. Pongracz Krisztian Kvell Krisztian Kvell |
| author_facet | Krisztina Banfai Krisztina Banfai David Ernszt David Ernszt Attila Pap Peter Bai Peter Bai Peter Bai Peter Bai Kitti Garai Kitti Garai Djeda Belharazem Judit E. Pongracz Judit E. Pongracz Krisztian Kvell Krisztian Kvell |
| author_sort | Krisztina Banfai |
| collection | DOAJ |
| description | With thymic senescence the epithelial network shrinks to be replaced by adipose tissue. Transcription factor TBX-1 controls thymus organogenesis, however, the same TBX-1 has also been reported to orchestrate beige adipose tissue development. Given these different roles of TBX-1, we have assessed if thymic TBX-1 expression persists and demonstrates this dualism during adulthood. We have also checked whether thymic adipose involution could yield beige adipose tissue. We have used adult mouse and human thymus tissue from various ages to evaluate the kinetics of TBX-1 expression, as well as mouse (TEP1) and human (1889c) thymic epithelial cells (TECs) for our studies. Electron micrographs show multi-locular lipid deposits typical of beige adipose cells. Histology staining shows the accumulation of neutral lipid deposits. qPCR measurements show persistent and/or elevating levels of beige-specific and beige-indicative markers (TBX-1, EAR-2, UCP-1, PPAR-gamma). We have performed miRNome profiling using qPCR-based QuantStudio platform and amplification-free NanoString platform. We have observed characteristic alterations, including increased miR21 level (promoting adipose tissue development) and decreased miR34a level (bias toward beige adipose tissue differentiation). Finally, using the Seahorse metabolic platform we have recorded a metabolic profile (OCR/ECAR ratio) indicative of beige adipose tissue. In summary, our results support that thymic adipose tissue emerging with senescence is bona fide beige adipose tissue. Our data show how the borders blur between a key immune tissue (the thymus) and a key metabolic tissue (beige adipose tissue) with senescence. Our work contributes to the understanding of cross talk between the immune system and metabolism. |
| first_indexed | 2024-12-11T20:49:13Z |
| format | Article |
| id | doaj.art-9401acb00de14d92b3c01e80cd74c2a2 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-11T20:49:13Z |
| publishDate | 2019-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-9401acb00de14d92b3c01e80cd74c2a22022-12-22T00:51:16ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922019-06-011010.3389/fendo.2019.00369459412“Beige” Cross Talk Between the Immune System and MetabolismKrisztina Banfai0Krisztina Banfai1David Ernszt2David Ernszt3Attila Pap4Peter Bai5Peter Bai6Peter Bai7Peter Bai8Kitti Garai9Kitti Garai10Djeda Belharazem11Judit E. Pongracz12Judit E. Pongracz13Krisztian Kvell14Krisztian Kvell15Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Pécs, Pécs, HungarySzentagothai Research Center, University of Pécs, Pécs, HungarySzentagothai Research Center, University of Pécs, Pécs, HungaryDepartment of Physiology, Medical School, University of Pécs, Pécs, HungaryDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen, HungaryMedical Chemistry, Faculty of Medicine, University of Debrecen, Debrecen, HungaryMTA-DE Cell Biology and Signaling Research Group, Debrecen, HungaryMTA-DE Lendulet Laboratory of Cellular Metabolism, Debrecen, HungaryResearch Center for Molecular Medicine, University of Debrecen, Debrecen, HungaryDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Pécs, Pécs, HungarySzentagothai Research Center, University of Pécs, Pécs, HungaryDepartment of Pathology, University Hospital of Mannheim, Mannheim, GermanyDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Pécs, Pécs, HungarySzentagothai Research Center, University of Pécs, Pécs, HungaryDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Pécs, Pécs, HungarySzentagothai Research Center, University of Pécs, Pécs, HungaryWith thymic senescence the epithelial network shrinks to be replaced by adipose tissue. Transcription factor TBX-1 controls thymus organogenesis, however, the same TBX-1 has also been reported to orchestrate beige adipose tissue development. Given these different roles of TBX-1, we have assessed if thymic TBX-1 expression persists and demonstrates this dualism during adulthood. We have also checked whether thymic adipose involution could yield beige adipose tissue. We have used adult mouse and human thymus tissue from various ages to evaluate the kinetics of TBX-1 expression, as well as mouse (TEP1) and human (1889c) thymic epithelial cells (TECs) for our studies. Electron micrographs show multi-locular lipid deposits typical of beige adipose cells. Histology staining shows the accumulation of neutral lipid deposits. qPCR measurements show persistent and/or elevating levels of beige-specific and beige-indicative markers (TBX-1, EAR-2, UCP-1, PPAR-gamma). We have performed miRNome profiling using qPCR-based QuantStudio platform and amplification-free NanoString platform. We have observed characteristic alterations, including increased miR21 level (promoting adipose tissue development) and decreased miR34a level (bias toward beige adipose tissue differentiation). Finally, using the Seahorse metabolic platform we have recorded a metabolic profile (OCR/ECAR ratio) indicative of beige adipose tissue. In summary, our results support that thymic adipose tissue emerging with senescence is bona fide beige adipose tissue. Our data show how the borders blur between a key immune tissue (the thymus) and a key metabolic tissue (beige adipose tissue) with senescence. Our work contributes to the understanding of cross talk between the immune system and metabolism.https://www.frontiersin.org/article/10.3389/fendo.2019.00369/fullthymus senescencebeige adipose tissueTBX-1UCP-1PPARgamma |
| spellingShingle | Krisztina Banfai Krisztina Banfai David Ernszt David Ernszt Attila Pap Peter Bai Peter Bai Peter Bai Peter Bai Kitti Garai Kitti Garai Djeda Belharazem Judit E. Pongracz Judit E. Pongracz Krisztian Kvell Krisztian Kvell “Beige” Cross Talk Between the Immune System and Metabolism Frontiers in Endocrinology thymus senescence beige adipose tissue TBX-1 UCP-1 PPARgamma |
| title | “Beige” Cross Talk Between the Immune System and Metabolism |
| title_full | “Beige” Cross Talk Between the Immune System and Metabolism |
| title_fullStr | “Beige” Cross Talk Between the Immune System and Metabolism |
| title_full_unstemmed | “Beige” Cross Talk Between the Immune System and Metabolism |
| title_short | “Beige” Cross Talk Between the Immune System and Metabolism |
| title_sort | beige cross talk between the immune system and metabolism |
| topic | thymus senescence beige adipose tissue TBX-1 UCP-1 PPARgamma |
| url | https://www.frontiersin.org/article/10.3389/fendo.2019.00369/full |
| work_keys_str_mv | AT krisztinabanfai beigecrosstalkbetweentheimmunesystemandmetabolism AT krisztinabanfai beigecrosstalkbetweentheimmunesystemandmetabolism AT davidernszt beigecrosstalkbetweentheimmunesystemandmetabolism AT davidernszt beigecrosstalkbetweentheimmunesystemandmetabolism AT attilapap beigecrosstalkbetweentheimmunesystemandmetabolism AT peterbai beigecrosstalkbetweentheimmunesystemandmetabolism AT peterbai beigecrosstalkbetweentheimmunesystemandmetabolism AT peterbai beigecrosstalkbetweentheimmunesystemandmetabolism AT peterbai beigecrosstalkbetweentheimmunesystemandmetabolism AT kittigarai beigecrosstalkbetweentheimmunesystemandmetabolism AT kittigarai beigecrosstalkbetweentheimmunesystemandmetabolism AT djedabelharazem beigecrosstalkbetweentheimmunesystemandmetabolism AT juditepongracz beigecrosstalkbetweentheimmunesystemandmetabolism AT juditepongracz beigecrosstalkbetweentheimmunesystemandmetabolism AT krisztiankvell beigecrosstalkbetweentheimmunesystemandmetabolism AT krisztiankvell beigecrosstalkbetweentheimmunesystemandmetabolism |