HPV and Other Risk Factors Involved in Pharyngeal Neoplasm—Clinical and Morphopathological Correlations in the Southwestern Region of Romania
Oropharyngeal squamous cell carcinoma (OPSCC) development is strongly associated with risk factors like smoking, chronic alcohol consumption, and the living environment, but also chronic human papilloma virus (HPV) infection, which can trigger cascade cellular changes leading to a neoplastic transfo...
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| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/8/984 |
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| author | Carmen Aurelia Mogoantă Mircea Sorin Ciolofan Anca-Maria Istrate-Ofițeru Stelian-Ștefăniță Mogoantă Gabriela-Camelia Roșu Florin Anghelina Alina-Nicoleta Căpitanescu Ioana Cristina Opriscan Nina Ionovici Mihaela Roxana Mitroi Oana Badea Gheorghe Iovănescu |
| author_facet | Carmen Aurelia Mogoantă Mircea Sorin Ciolofan Anca-Maria Istrate-Ofițeru Stelian-Ștefăniță Mogoantă Gabriela-Camelia Roșu Florin Anghelina Alina-Nicoleta Căpitanescu Ioana Cristina Opriscan Nina Ionovici Mihaela Roxana Mitroi Oana Badea Gheorghe Iovănescu |
| author_sort | Carmen Aurelia Mogoantă |
| collection | DOAJ |
| description | Oropharyngeal squamous cell carcinoma (OPSCC) development is strongly associated with risk factors like smoking, chronic alcohol consumption, and the living environment, but also chronic human papilloma virus (HPV) infection, which can trigger cascade cellular changes leading to a neoplastic transformation. The prevalence of these factors differs among different world regions, and the prevention, diagnosis, and prognosis of OPSCC are highly dependent on them. We performed a retrospective study on 406 patients diagnosed with OPSCC in our region that were classified according to the tumor type, localization and diagnosis stage, demographic characteristics, risk factors, and histological and immunohistochemical features. We found that most of the patients were men from urban areas with a smoking habit, while most of the women in our study were diagnosed with tonsillar OPSCC and had a history of chronic alcoholism. During the immunohistochemical study, we analyzed the tumor immunoreactivity against anti-p16 and anti-HPV antibodies as markers of HPV involvement in tumor progression, as well as the correlation with the percentage of intratumoral nuclei immunomarked with the anti-Ki 67 antibody in serial samples. We observed that the percentage of Ki67-positive nuclei increased proportionally with the presence of intratumoral HPV; thus, active HPV infection leads to an increase in the rate of tumor progression. Our results support the implementation of strategies for OPSCC prevention and early diagnosis and can be a starting point for future studies aiming at adapting surgical and oncological treatment according to the HPV stage for better therapeutic results. |
| first_indexed | 2024-03-10T23:40:40Z |
| format | Article |
| id | doaj.art-940386eecf27471ba16e7fdb94df8c80 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T23:40:40Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-940386eecf27471ba16e7fdb94df8c802023-11-19T02:31:45ZengMDPI AGPathogens2076-08172023-07-0112898410.3390/pathogens12080984HPV and Other Risk Factors Involved in Pharyngeal Neoplasm—Clinical and Morphopathological Correlations in the Southwestern Region of RomaniaCarmen Aurelia Mogoantă0Mircea Sorin Ciolofan1Anca-Maria Istrate-Ofițeru2Stelian-Ștefăniță Mogoantă3Gabriela-Camelia Roșu4Florin Anghelina5Alina-Nicoleta Căpitanescu6Ioana Cristina Opriscan7Nina Ionovici8Mihaela Roxana Mitroi9Oana Badea10Gheorghe Iovănescu11ENT Department, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaENT Department, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaHistology Department, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaGeneral Surgery Department, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaHistology Department, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaENT Department, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaENT Department, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDoctoral School, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Occupational Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaENT Department, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Modern Languages, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaENT Department, Faculty of Medicine, University of Medicine and Pharmacy Victor Babes, 300041 Timisoara, RomaniaOropharyngeal squamous cell carcinoma (OPSCC) development is strongly associated with risk factors like smoking, chronic alcohol consumption, and the living environment, but also chronic human papilloma virus (HPV) infection, which can trigger cascade cellular changes leading to a neoplastic transformation. The prevalence of these factors differs among different world regions, and the prevention, diagnosis, and prognosis of OPSCC are highly dependent on them. We performed a retrospective study on 406 patients diagnosed with OPSCC in our region that were classified according to the tumor type, localization and diagnosis stage, demographic characteristics, risk factors, and histological and immunohistochemical features. We found that most of the patients were men from urban areas with a smoking habit, while most of the women in our study were diagnosed with tonsillar OPSCC and had a history of chronic alcoholism. During the immunohistochemical study, we analyzed the tumor immunoreactivity against anti-p16 and anti-HPV antibodies as markers of HPV involvement in tumor progression, as well as the correlation with the percentage of intratumoral nuclei immunomarked with the anti-Ki 67 antibody in serial samples. We observed that the percentage of Ki67-positive nuclei increased proportionally with the presence of intratumoral HPV; thus, active HPV infection leads to an increase in the rate of tumor progression. Our results support the implementation of strategies for OPSCC prevention and early diagnosis and can be a starting point for future studies aiming at adapting surgical and oncological treatment according to the HPV stage for better therapeutic results.https://www.mdpi.com/2076-0817/12/8/984human papillomaviruscell proliferationtumor suppressor gene |
| spellingShingle | Carmen Aurelia Mogoantă Mircea Sorin Ciolofan Anca-Maria Istrate-Ofițeru Stelian-Ștefăniță Mogoantă Gabriela-Camelia Roșu Florin Anghelina Alina-Nicoleta Căpitanescu Ioana Cristina Opriscan Nina Ionovici Mihaela Roxana Mitroi Oana Badea Gheorghe Iovănescu HPV and Other Risk Factors Involved in Pharyngeal Neoplasm—Clinical and Morphopathological Correlations in the Southwestern Region of Romania Pathogens human papillomavirus cell proliferation tumor suppressor gene |
| title | HPV and Other Risk Factors Involved in Pharyngeal Neoplasm—Clinical and Morphopathological Correlations in the Southwestern Region of Romania |
| title_full | HPV and Other Risk Factors Involved in Pharyngeal Neoplasm—Clinical and Morphopathological Correlations in the Southwestern Region of Romania |
| title_fullStr | HPV and Other Risk Factors Involved in Pharyngeal Neoplasm—Clinical and Morphopathological Correlations in the Southwestern Region of Romania |
| title_full_unstemmed | HPV and Other Risk Factors Involved in Pharyngeal Neoplasm—Clinical and Morphopathological Correlations in the Southwestern Region of Romania |
| title_short | HPV and Other Risk Factors Involved in Pharyngeal Neoplasm—Clinical and Morphopathological Correlations in the Southwestern Region of Romania |
| title_sort | hpv and other risk factors involved in pharyngeal neoplasm clinical and morphopathological correlations in the southwestern region of romania |
| topic | human papillomavirus cell proliferation tumor suppressor gene |
| url | https://www.mdpi.com/2076-0817/12/8/984 |
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