Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization
Angelo Gismondi,1 Valentina Nanni,1 Giacomo Reina,2 Silvia Orlanducci,2 Maria Letizia Terranova,2 Antonella Canini1 1Department of Biology, 2Department of Chemical Science and Technology, University of Rome “Tor Vergata”, Rome, Italy Abstract: For the first time, we coupled red...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2016-02-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/nanodiamonds-coupled-with-57-dimethoxycoumarin-a-plant-bioactive-metab-peer-reviewed-article-IJN |
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| author | Gismondi A Nanni V Reina G Orlanducci S Terranova ML Canini A |
| author_facet | Gismondi A Nanni V Reina G Orlanducci S Terranova ML Canini A |
| author_sort | Gismondi A |
| collection | DOAJ |
| description | Angelo Gismondi,1 Valentina Nanni,1 Giacomo Reina,2 Silvia Orlanducci,2 Maria Letizia Terranova,2 Antonella Canini1 1Department of Biology, 2Department of Chemical Science and Technology, University of Rome “Tor Vergata”, Rome, Italy Abstract: For the first time, we coupled reduced detonation nanodiamonds (NDs) with a plant secondary metabolite, citropten (5,7-dimethoxycoumarin), and demonstrated how this complex was able to reduce B16F10 tumor cell growth more effectively than treatment with the pure molecule. These results encouraged us to find out the specific mechanism underlying this phenomenon. Internalization kinetics and quantification of citropten in cells after treatment with its pure or ND-conjugated form were measured, and it was revealed that the coupling between NDs and citropten was essential for the biological properties of the complex. We showed that the adduct was not able to induce apoptosis, senescence, or differentiation, but it determined cell cycle arrest, morphological changes, and alteration of mRNA levels of the cytoskeletal-related genes. The identification of metaphasic nuclei and irregular disposition of β-actin in the cell cytoplasm supported the hypothesis that citropten conjugated with NDs showed antimitotic properties in B16F10 cells. This work can be considered a pioneering piece of research that could promote and support the biomedical use of plant drug-functionalized NDs in cancer therapy. Keywords: citropten, cytoskeletal structure, plant secondary metabolite, melanoma, internalization kinetics |
| first_indexed | 2024-12-19T08:50:05Z |
| format | Article |
| id | doaj.art-9403db7aea0c4d94b450abfff03a6174 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-19T08:50:05Z |
| publishDate | 2016-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-9403db7aea0c4d94b450abfff03a61742022-12-21T20:28:44ZengDove Medical PressInternational Journal of Nanomedicine1178-20132016-02-012016Issue 155757425466Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organizationGismondi ANanni VReina GOrlanducci STerranova MLCanini AAngelo Gismondi,1 Valentina Nanni,1 Giacomo Reina,2 Silvia Orlanducci,2 Maria Letizia Terranova,2 Antonella Canini1 1Department of Biology, 2Department of Chemical Science and Technology, University of Rome “Tor Vergata”, Rome, Italy Abstract: For the first time, we coupled reduced detonation nanodiamonds (NDs) with a plant secondary metabolite, citropten (5,7-dimethoxycoumarin), and demonstrated how this complex was able to reduce B16F10 tumor cell growth more effectively than treatment with the pure molecule. These results encouraged us to find out the specific mechanism underlying this phenomenon. Internalization kinetics and quantification of citropten in cells after treatment with its pure or ND-conjugated form were measured, and it was revealed that the coupling between NDs and citropten was essential for the biological properties of the complex. We showed that the adduct was not able to induce apoptosis, senescence, or differentiation, but it determined cell cycle arrest, morphological changes, and alteration of mRNA levels of the cytoskeletal-related genes. The identification of metaphasic nuclei and irregular disposition of β-actin in the cell cytoplasm supported the hypothesis that citropten conjugated with NDs showed antimitotic properties in B16F10 cells. This work can be considered a pioneering piece of research that could promote and support the biomedical use of plant drug-functionalized NDs in cancer therapy. Keywords: citropten, cytoskeletal structure, plant secondary metabolite, melanoma, internalization kineticshttps://www.dovepress.com/nanodiamonds-coupled-with-57-dimethoxycoumarin-a-plant-bioactive-metab-peer-reviewed-article-IJNCitroptencytoskeletal structureplant secondary metabolitemelanomainternalization kinetics. |
| spellingShingle | Gismondi A Nanni V Reina G Orlanducci S Terranova ML Canini A Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization International Journal of Nanomedicine Citropten cytoskeletal structure plant secondary metabolite melanoma internalization kinetics. |
| title | Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization |
| title_full | Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization |
| title_fullStr | Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization |
| title_full_unstemmed | Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization |
| title_short | Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization |
| title_sort | nanodiamonds coupled with 5 7 dimethoxycoumarin a plant bioactive metabolite interfere with the mitotic process in b16f10 cells altering the actin organization |
| topic | Citropten cytoskeletal structure plant secondary metabolite melanoma internalization kinetics. |
| url | https://www.dovepress.com/nanodiamonds-coupled-with-57-dimethoxycoumarin-a-plant-bioactive-metab-peer-reviewed-article-IJN |
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