Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production
Abstract Rhodosporidium toruloides is a useful oleaginous yeast, but lipids production is affected by various factors including nutrients in the culture medium. Herein, the R-ZL2 high-yield mutant strain was used to investigate the effects of different carbon sources (sucrose, glucose, xylose), nitr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-11-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-021-01313-6 |
| _version_ | 1818743371373477888 |
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| author | Zi Ye Tongrui Sun Huoye Hao Yanling He Xueyan Liu Minrui Guo Guogang Chen |
| author_facet | Zi Ye Tongrui Sun Huoye Hao Yanling He Xueyan Liu Minrui Guo Guogang Chen |
| author_sort | Zi Ye |
| collection | DOAJ |
| description | Abstract Rhodosporidium toruloides is a useful oleaginous yeast, but lipids production is affected by various factors including nutrients in the culture medium. Herein, the R-ZL2 high-yield mutant strain was used to investigate the effects of different carbon sources (sucrose, glucose, xylose), nitrogen sources (ammonium sulphate, ammonium nitrate), and C/N ratio on lipids production capacity, get the following conclusion (1) Compared with glucose and xylose, sucrose was a superior carbon source for lipids production; (2) When using ammonium sulphate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (10.7 g/L, 6.32 g/L and 59%, respectively), and lipids produced under different C/N conditions have potential for biodiesel production (except for C/N = 40 and C/N = 80); (3) When using ammonium nitrate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (12.1 g/L, 8.25 g/L and 65%, respectively), and lipids produced under different C/N ratio conditions have potential for biodiesel production. Thus, a combination of sucrose and ammonium nitrate was optimal for the lipid accumulation in R-ZL2. The findings will lay a foundation for further improving lipids yields. |
| first_indexed | 2024-12-18T02:27:21Z |
| format | Article |
| id | doaj.art-9403f59437d44bca847a3b0859f6079e |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-18T02:27:21Z |
| publishDate | 2021-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-9403f59437d44bca847a3b0859f6079e2022-12-21T21:24:00ZengSpringerOpenAMB Express2191-08552021-11-0111111110.1186/s13568-021-01313-6Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids productionZi Ye0Tongrui Sun1Huoye Hao2Yanling He3Xueyan Liu4Minrui Guo5Guogang Chen6School of Food Science and Technology, College of Food, Shihezi UniversitySchool of Food Science and Technology, College of Food, Shihezi UniversitySchool of Food Science and Technology, College of Food, Shihezi UniversitySchool of Food Science and Technology, College of Food, Shihezi UniversitySchool of Food Science and Technology, College of Food, Shihezi UniversitySchool of Food Science and Technology, College of Food, Shihezi UniversitySchool of Food Science and Technology, College of Food, Shihezi UniversityAbstract Rhodosporidium toruloides is a useful oleaginous yeast, but lipids production is affected by various factors including nutrients in the culture medium. Herein, the R-ZL2 high-yield mutant strain was used to investigate the effects of different carbon sources (sucrose, glucose, xylose), nitrogen sources (ammonium sulphate, ammonium nitrate), and C/N ratio on lipids production capacity, get the following conclusion (1) Compared with glucose and xylose, sucrose was a superior carbon source for lipids production; (2) When using ammonium sulphate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (10.7 g/L, 6.32 g/L and 59%, respectively), and lipids produced under different C/N conditions have potential for biodiesel production (except for C/N = 40 and C/N = 80); (3) When using ammonium nitrate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (12.1 g/L, 8.25 g/L and 65%, respectively), and lipids produced under different C/N ratio conditions have potential for biodiesel production. Thus, a combination of sucrose and ammonium nitrate was optimal for the lipid accumulation in R-ZL2. The findings will lay a foundation for further improving lipids yields.https://doi.org/10.1186/s13568-021-01313-6Carbon sourcesC/N ratioLipid productionNitrogen sourcesRhodosporidium toruloides |
| spellingShingle | Zi Ye Tongrui Sun Huoye Hao Yanling He Xueyan Liu Minrui Guo Guogang Chen Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production AMB Express Carbon sources C/N ratio Lipid production Nitrogen sources Rhodosporidium toruloides |
| title | Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production |
| title_full | Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production |
| title_fullStr | Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production |
| title_full_unstemmed | Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production |
| title_short | Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production |
| title_sort | optimising nutrients in the culture medium of rhodosporidium toruloides enhances lipids production |
| topic | Carbon sources C/N ratio Lipid production Nitrogen sources Rhodosporidium toruloides |
| url | https://doi.org/10.1186/s13568-021-01313-6 |
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