Carbapenem-Resistant <i>Acinetobacter baumannii</i>: Biofilm-Associated Genes, Biofilm-Eradication Potential of Disinfectants, and Biofilm-Inhibitory Effects of Selenium Nanoparticles
This study aimed to investigate the biofilm-production ability of carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB), the biofilm-eradication potential of 70% ethanol and 0.5% sodium hypochlorite, the effects of selenium nanoparticles (SeNPs) against planktonic and biofilm-embedd...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/11/1/171 |
| _version_ | 1797438536526331904 |
|---|---|
| author | Aleksandra Smitran Bojana Lukovic LJiljana Bozic Dijana Jelic Milos Jovicevic Jovana Kabic Dusan Kekic Jovana Ranin Natasa Opavski Ina Gajic |
| author_facet | Aleksandra Smitran Bojana Lukovic LJiljana Bozic Dijana Jelic Milos Jovicevic Jovana Kabic Dusan Kekic Jovana Ranin Natasa Opavski Ina Gajic |
| author_sort | Aleksandra Smitran |
| collection | DOAJ |
| description | This study aimed to investigate the biofilm-production ability of carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB), the biofilm-eradication potential of 70% ethanol and 0.5% sodium hypochlorite, the effects of selenium nanoparticles (SeNPs) against planktonic and biofilm-embedded CRAB, and the relationship between biofilm production and bacterial genotypes. A total of 111 CRAB isolates were tested for antimicrobial susceptibility, biofilm formation, presence of the genes encoding carbapenemases, and biofilm-associated virulence factors. The antibiofilm effects of disinfectants and SeNPs against CRAB isolates were also tested. The vast majority of the tested isolates were biofilm producers (91.9%). The <i>bap</i>, <i>ompA</i>, and <i>csuE</i> genes were found in 57%, 70%, and 76% of the CRAB isolates, with the <i>csuE</i> being significantly more common among biofilm producers (78.6%) compared to non-biofilm-producing CRAB (25%). The tested disinfectants showed a better antibiofilm effect on moderate and strong biofilm producers than on weak producers (<i>p</i> < 0.01). The SeNPs showed an inhibitory effect against all tested planktonic (MIC range: 0.00015 to >1.25 mg/mL) and biofilm-embedded CRAB, with a minimum biofilm inhibitory concentration of less than 0.15 mg/mL for 90% of biofilm producers. In conclusion, SeNPs might be used as promising therapeutic and medical device coating agents, thus serving as an alternative approach for the prevention of biofilm-related infections. |
| first_indexed | 2024-03-09T11:38:22Z |
| format | Article |
| id | doaj.art-9407709fd76d45d3ab4149aa86707f27 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T11:38:22Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-9407709fd76d45d3ab4149aa86707f272023-11-30T23:37:27ZengMDPI AGMicroorganisms2076-26072023-01-0111117110.3390/microorganisms11010171Carbapenem-Resistant <i>Acinetobacter baumannii</i>: Biofilm-Associated Genes, Biofilm-Eradication Potential of Disinfectants, and Biofilm-Inhibitory Effects of Selenium NanoparticlesAleksandra Smitran0Bojana Lukovic1LJiljana Bozic2Dijana Jelic3Milos Jovicevic4Jovana Kabic5Dusan Kekic6Jovana Ranin7Natasa Opavski8Ina Gajic9Faculty of Medicine, University of Banja Luka, 78 000 Banja Luka, Bosnia and HerzegovinaAcademy of Applied Studies Belgrade, College of Health Sciences, 11000 Belgrade, SerbiaFaculty of Medicine, University of Banja Luka, 78 000 Banja Luka, Bosnia and HerzegovinaDepartment of Chemistry, Faculty of Natural Sciences and Mathematics, University of Banja Luka, 78 000 Banja Luka, Bosnia and HerzegovinaInstitute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, SerbiaInstitute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, SerbiaInstitute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, SerbiaInstitute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, SerbiaInstitute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, SerbiaInstitute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, SerbiaThis study aimed to investigate the biofilm-production ability of carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB), the biofilm-eradication potential of 70% ethanol and 0.5% sodium hypochlorite, the effects of selenium nanoparticles (SeNPs) against planktonic and biofilm-embedded CRAB, and the relationship between biofilm production and bacterial genotypes. A total of 111 CRAB isolates were tested for antimicrobial susceptibility, biofilm formation, presence of the genes encoding carbapenemases, and biofilm-associated virulence factors. The antibiofilm effects of disinfectants and SeNPs against CRAB isolates were also tested. The vast majority of the tested isolates were biofilm producers (91.9%). The <i>bap</i>, <i>ompA</i>, and <i>csuE</i> genes were found in 57%, 70%, and 76% of the CRAB isolates, with the <i>csuE</i> being significantly more common among biofilm producers (78.6%) compared to non-biofilm-producing CRAB (25%). The tested disinfectants showed a better antibiofilm effect on moderate and strong biofilm producers than on weak producers (<i>p</i> < 0.01). The SeNPs showed an inhibitory effect against all tested planktonic (MIC range: 0.00015 to >1.25 mg/mL) and biofilm-embedded CRAB, with a minimum biofilm inhibitory concentration of less than 0.15 mg/mL for 90% of biofilm producers. In conclusion, SeNPs might be used as promising therapeutic and medical device coating agents, thus serving as an alternative approach for the prevention of biofilm-related infections.https://www.mdpi.com/2076-2607/11/1/171carbapenem-resistant <i>Acinetobacter baumannii</i>disinfectantsbiofilm-associated genesselenium nanoparticles |
| spellingShingle | Aleksandra Smitran Bojana Lukovic LJiljana Bozic Dijana Jelic Milos Jovicevic Jovana Kabic Dusan Kekic Jovana Ranin Natasa Opavski Ina Gajic Carbapenem-Resistant <i>Acinetobacter baumannii</i>: Biofilm-Associated Genes, Biofilm-Eradication Potential of Disinfectants, and Biofilm-Inhibitory Effects of Selenium Nanoparticles Microorganisms carbapenem-resistant <i>Acinetobacter baumannii</i> disinfectants biofilm-associated genes selenium nanoparticles |
| title | Carbapenem-Resistant <i>Acinetobacter baumannii</i>: Biofilm-Associated Genes, Biofilm-Eradication Potential of Disinfectants, and Biofilm-Inhibitory Effects of Selenium Nanoparticles |
| title_full | Carbapenem-Resistant <i>Acinetobacter baumannii</i>: Biofilm-Associated Genes, Biofilm-Eradication Potential of Disinfectants, and Biofilm-Inhibitory Effects of Selenium Nanoparticles |
| title_fullStr | Carbapenem-Resistant <i>Acinetobacter baumannii</i>: Biofilm-Associated Genes, Biofilm-Eradication Potential of Disinfectants, and Biofilm-Inhibitory Effects of Selenium Nanoparticles |
| title_full_unstemmed | Carbapenem-Resistant <i>Acinetobacter baumannii</i>: Biofilm-Associated Genes, Biofilm-Eradication Potential of Disinfectants, and Biofilm-Inhibitory Effects of Selenium Nanoparticles |
| title_short | Carbapenem-Resistant <i>Acinetobacter baumannii</i>: Biofilm-Associated Genes, Biofilm-Eradication Potential of Disinfectants, and Biofilm-Inhibitory Effects of Selenium Nanoparticles |
| title_sort | carbapenem resistant i acinetobacter baumannii i biofilm associated genes biofilm eradication potential of disinfectants and biofilm inhibitory effects of selenium nanoparticles |
| topic | carbapenem-resistant <i>Acinetobacter baumannii</i> disinfectants biofilm-associated genes selenium nanoparticles |
| url | https://www.mdpi.com/2076-2607/11/1/171 |
| work_keys_str_mv | AT aleksandrasmitran carbapenemresistantiacinetobacterbaumanniiibiofilmassociatedgenesbiofilmeradicationpotentialofdisinfectantsandbiofilminhibitoryeffectsofseleniumnanoparticles AT bojanalukovic carbapenemresistantiacinetobacterbaumanniiibiofilmassociatedgenesbiofilmeradicationpotentialofdisinfectantsandbiofilminhibitoryeffectsofseleniumnanoparticles AT ljiljanabozic carbapenemresistantiacinetobacterbaumanniiibiofilmassociatedgenesbiofilmeradicationpotentialofdisinfectantsandbiofilminhibitoryeffectsofseleniumnanoparticles AT dijanajelic carbapenemresistantiacinetobacterbaumanniiibiofilmassociatedgenesbiofilmeradicationpotentialofdisinfectantsandbiofilminhibitoryeffectsofseleniumnanoparticles AT milosjovicevic carbapenemresistantiacinetobacterbaumanniiibiofilmassociatedgenesbiofilmeradicationpotentialofdisinfectantsandbiofilminhibitoryeffectsofseleniumnanoparticles AT jovanakabic carbapenemresistantiacinetobacterbaumanniiibiofilmassociatedgenesbiofilmeradicationpotentialofdisinfectantsandbiofilminhibitoryeffectsofseleniumnanoparticles AT dusankekic carbapenemresistantiacinetobacterbaumanniiibiofilmassociatedgenesbiofilmeradicationpotentialofdisinfectantsandbiofilminhibitoryeffectsofseleniumnanoparticles AT jovanaranin carbapenemresistantiacinetobacterbaumanniiibiofilmassociatedgenesbiofilmeradicationpotentialofdisinfectantsandbiofilminhibitoryeffectsofseleniumnanoparticles AT natasaopavski carbapenemresistantiacinetobacterbaumanniiibiofilmassociatedgenesbiofilmeradicationpotentialofdisinfectantsandbiofilminhibitoryeffectsofseleniumnanoparticles AT inagajic carbapenemresistantiacinetobacterbaumanniiibiofilmassociatedgenesbiofilmeradicationpotentialofdisinfectantsandbiofilminhibitoryeffectsofseleniumnanoparticles |