Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model Mice
Background: Near-infrared photoimmunotherapy (NIR-PIT) is a cancer therapy that causes an increase in tumor perfusion, a phenomenon termed the super-enhanced permeability and retention effect. Currently, in vivo treatment efficacy of NIR-PIT is observable days after treatment, but monitoring would b...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publications
2020-06-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1177/1536012120934965 |
| _version_ | 1797288048057122816 |
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| author | Adrian Rosenberg Daiki Fujimura MD Ryuhei Okada MD, PhD Aki Furusawa PhD Fuyuki Inagaki MD, PhD Hiroaki Wakiyama MD, PhD Takuya Kato MD, PhD Peter L. Choyke MD Hisataka Kobayashi MD, PhD |
| author_facet | Adrian Rosenberg Daiki Fujimura MD Ryuhei Okada MD, PhD Aki Furusawa PhD Fuyuki Inagaki MD, PhD Hiroaki Wakiyama MD, PhD Takuya Kato MD, PhD Peter L. Choyke MD Hisataka Kobayashi MD, PhD |
| author_sort | Adrian Rosenberg |
| collection | DOAJ |
| description | Background: Near-infrared photoimmunotherapy (NIR-PIT) is a cancer therapy that causes an increase in tumor perfusion, a phenomenon termed the super-enhanced permeability and retention effect. Currently, in vivo treatment efficacy of NIR-PIT is observable days after treatment, but monitoring would be improved by more acute detection of intratumor change. Fluorescence imaging may detect increased tumor perfusion immediately after treatment. Methods: In the first experiment, athymic nude mouse models bearing unilateral subcutaneous flank tumors were treated with either NIR-PIT or laser therapy only. In the second experiment, mice bearing bilateral flank tumors were treated with NIR-PIT only on the left-sided tumor. In both groups, immediately after treatment, indocyanine green was injected at different doses intravenously, and mice were monitored with the Shimadzu LIGHTVISION fluorescence imaging system for 1 hour. Results: Tumor-to-background ratio of fluorescence intensity increased over the 60 minutes of monitoring in treated mice but did not vary significantly in control mice. Tumor-to-background ratio was highest in the 1 mg kg −1 and 0.3 mg kg −1 doses. In mice with bilateral tumors, tumor-to-untreated tumor ratio increased similarly. Conclusions: Acute changes in tumor perfusion after NIR-PIT can be detected by real-time fluorescence imaging. |
| first_indexed | 2024-03-07T18:43:38Z |
| format | Article |
| id | doaj.art-940fa7e2050d4118b9a413114e40e0bd |
| institution | Directory Open Access Journal |
| issn | 1536-0121 |
| language | English |
| last_indexed | 2024-03-07T18:43:38Z |
| publishDate | 2020-06-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj.art-940fa7e2050d4118b9a413114e40e0bd2024-03-02T03:15:50ZengSAGE PublicationsMolecular Imaging1536-01212020-06-011910.1177/1536012120934965Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model MiceAdrian Rosenberg0Daiki Fujimura MD1Ryuhei Okada MD, PhD2Aki Furusawa PhD3Fuyuki Inagaki MD, PhD4Hiroaki Wakiyama MD, PhD5Takuya Kato MD, PhD6Peter L. Choyke MD7Hisataka Kobayashi MD, PhD8 Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USABackground: Near-infrared photoimmunotherapy (NIR-PIT) is a cancer therapy that causes an increase in tumor perfusion, a phenomenon termed the super-enhanced permeability and retention effect. Currently, in vivo treatment efficacy of NIR-PIT is observable days after treatment, but monitoring would be improved by more acute detection of intratumor change. Fluorescence imaging may detect increased tumor perfusion immediately after treatment. Methods: In the first experiment, athymic nude mouse models bearing unilateral subcutaneous flank tumors were treated with either NIR-PIT or laser therapy only. In the second experiment, mice bearing bilateral flank tumors were treated with NIR-PIT only on the left-sided tumor. In both groups, immediately after treatment, indocyanine green was injected at different doses intravenously, and mice were monitored with the Shimadzu LIGHTVISION fluorescence imaging system for 1 hour. Results: Tumor-to-background ratio of fluorescence intensity increased over the 60 minutes of monitoring in treated mice but did not vary significantly in control mice. Tumor-to-background ratio was highest in the 1 mg kg −1 and 0.3 mg kg −1 doses. In mice with bilateral tumors, tumor-to-untreated tumor ratio increased similarly. Conclusions: Acute changes in tumor perfusion after NIR-PIT can be detected by real-time fluorescence imaging.https://doi.org/10.1177/1536012120934965 |
| spellingShingle | Adrian Rosenberg Daiki Fujimura MD Ryuhei Okada MD, PhD Aki Furusawa PhD Fuyuki Inagaki MD, PhD Hiroaki Wakiyama MD, PhD Takuya Kato MD, PhD Peter L. Choyke MD Hisataka Kobayashi MD, PhD Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model Mice Molecular Imaging |
| title | Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model Mice |
| title_full | Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model Mice |
| title_fullStr | Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model Mice |
| title_full_unstemmed | Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model Mice |
| title_short | Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model Mice |
| title_sort | real time fluorescence imaging using indocyanine green to assess therapeutic effects of near infrared photoimmunotherapy in tumor model mice |
| url | https://doi.org/10.1177/1536012120934965 |
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