Transcriptomic Study on the Lungs of Broilers with Ascites Syndrome
Although broiler ascites syndrome (AS) has been extensively studied, its pathogenesis remains unclear. The lack of cardiopulmonary function in broilers causes relative hypoxia in the body; hence, the lung is the main target organ of AS. However, the transcriptome of AS lung tissue in broilers has no...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/1/175 |
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| author | Dongqing Guo Jian Zhang Yufeng Han Liang Cui Huimin Wang Keyao Wang Peiqi Li Ruiqiang Deng Jie Kang Zhibian Duan |
| author_facet | Dongqing Guo Jian Zhang Yufeng Han Liang Cui Huimin Wang Keyao Wang Peiqi Li Ruiqiang Deng Jie Kang Zhibian Duan |
| author_sort | Dongqing Guo |
| collection | DOAJ |
| description | Although broiler ascites syndrome (AS) has been extensively studied, its pathogenesis remains unclear. The lack of cardiopulmonary function in broilers causes relative hypoxia in the body; hence, the lung is the main target organ of AS. However, the transcriptome of AS lung tissue in broilers has not been studied. In this study, an AS model was successfully constructed, and lung tissues of three AS broilers and three healthy broilers were obtained for RNA sequencing (RNA-seq) and pathological observation. The results showed that 614 genes were up-regulated and 828 genes were down-regulated in the AS group compared with the normal group. Gene Ontology (GO) functional annotation revealed the following up-regulated genes: <i>FABP4</i>, <i>APLN</i>, <i>EIF2AK4</i>, <i>HMOX1</i>, <i>MMP9</i>, <i>THBS1</i>, <i>TLR4</i>, <i>BCL2</i>; and down-regulated genes: <i>APELA</i>, <i>FGF7</i>, <i>WNT5A</i>, <i>CDK6</i>, <i>IL7</i>, <i>IL7R</i>, <i>APLNR</i>. These genes have attracted much attention in cardiovascular diseases such as pulmonary hypertension. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that multiple metabolic processes were enriched, indicating abnormal lung metabolism of AS in broilers. These findings elucidate the potential genes and signal pathways in the lungs of broilers with AS and provide a potential target for studying the pathogenesis and preventing AS. |
| first_indexed | 2024-03-11T10:09:03Z |
| format | Article |
| id | doaj.art-94147cb53ca546c5a003727f1e8e29dd |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T10:09:03Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-94147cb53ca546c5a003727f1e8e29dd2023-11-16T14:49:09ZengMDPI AGAnimals2076-26152023-01-0113117510.3390/ani13010175Transcriptomic Study on the Lungs of Broilers with Ascites SyndromeDongqing Guo0Jian Zhang1Yufeng Han2Liang Cui3Huimin Wang4Keyao Wang5Peiqi Li6Ruiqiang Deng7Jie Kang8Zhibian Duan9College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaAlthough broiler ascites syndrome (AS) has been extensively studied, its pathogenesis remains unclear. The lack of cardiopulmonary function in broilers causes relative hypoxia in the body; hence, the lung is the main target organ of AS. However, the transcriptome of AS lung tissue in broilers has not been studied. In this study, an AS model was successfully constructed, and lung tissues of three AS broilers and three healthy broilers were obtained for RNA sequencing (RNA-seq) and pathological observation. The results showed that 614 genes were up-regulated and 828 genes were down-regulated in the AS group compared with the normal group. Gene Ontology (GO) functional annotation revealed the following up-regulated genes: <i>FABP4</i>, <i>APLN</i>, <i>EIF2AK4</i>, <i>HMOX1</i>, <i>MMP9</i>, <i>THBS1</i>, <i>TLR4</i>, <i>BCL2</i>; and down-regulated genes: <i>APELA</i>, <i>FGF7</i>, <i>WNT5A</i>, <i>CDK6</i>, <i>IL7</i>, <i>IL7R</i>, <i>APLNR</i>. These genes have attracted much attention in cardiovascular diseases such as pulmonary hypertension. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that multiple metabolic processes were enriched, indicating abnormal lung metabolism of AS in broilers. These findings elucidate the potential genes and signal pathways in the lungs of broilers with AS and provide a potential target for studying the pathogenesis and preventing AS.https://www.mdpi.com/2076-2615/13/1/175broiler ascites syndromelungmetabolic hypoxiaRNA-seq |
| spellingShingle | Dongqing Guo Jian Zhang Yufeng Han Liang Cui Huimin Wang Keyao Wang Peiqi Li Ruiqiang Deng Jie Kang Zhibian Duan Transcriptomic Study on the Lungs of Broilers with Ascites Syndrome Animals broiler ascites syndrome lung metabolic hypoxia RNA-seq |
| title | Transcriptomic Study on the Lungs of Broilers with Ascites Syndrome |
| title_full | Transcriptomic Study on the Lungs of Broilers with Ascites Syndrome |
| title_fullStr | Transcriptomic Study on the Lungs of Broilers with Ascites Syndrome |
| title_full_unstemmed | Transcriptomic Study on the Lungs of Broilers with Ascites Syndrome |
| title_short | Transcriptomic Study on the Lungs of Broilers with Ascites Syndrome |
| title_sort | transcriptomic study on the lungs of broilers with ascites syndrome |
| topic | broiler ascites syndrome lung metabolic hypoxia RNA-seq |
| url | https://www.mdpi.com/2076-2615/13/1/175 |
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