Effect of Instant Controlled Pressure Drop (DIC) Treatment on the Detection of Nut Allergens by Real Time PCR
Tree nuts show nutritional properties and human health benefits. However, they contain allergenic proteins, which make them harmful to the sensitised population. The presence of tree nuts on food labelling is mandatory and, consequently, the development of suitable analytical methodologies to detect...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/6/729 |
| _version_ | 1797566310992838656 |
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| author | Africa Sanchiz Carmen Cuadrado Joseph Haddad Rosario Linacero |
| author_facet | Africa Sanchiz Carmen Cuadrado Joseph Haddad Rosario Linacero |
| author_sort | Africa Sanchiz |
| collection | DOAJ |
| description | Tree nuts show nutritional properties and human health benefits. However, they contain allergenic proteins, which make them harmful to the sensitised population. The presence of tree nuts on food labelling is mandatory and, consequently, the development of suitable analytical methodologies to detect nuts in processed foods is advisable. Real-Time PCR allowed a specific and accurate amplification of allergen sequences. Some food processing methods could induce structural and/or conformational changes in proteins by altering their allergenic capacity, as well as produce the fragmentation and/or degradation of genomic DNA. In this work, we analysed by means of Real-Time PCR, the influence of pressure and thermal processing through Instant Controlled Pressure Drop (DIC) on the detectability of hazelnut, pistachio and cashew allergens. The detection of targets in hazelnut, pistachio and cashew (Cor a 9, Pis v 1 and Ana o 1, respectively) is affected by the treatment to different extents depending on the tree nut. Results are compared to those previously obtained by our group in the analysis of different treatments on the amplificability of the same targets. Reduction in amplificability is similar to that reported for some autoclave conditions. Our assays might allow for the detection of up to 1000 mg/kg of hazelnut, pistachio and cashew flours after being submitted to DIC treatment in food matrices. |
| first_indexed | 2024-03-10T19:24:55Z |
| format | Article |
| id | doaj.art-941873284b5340feae3868e580ba4195 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T19:24:55Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-941873284b5340feae3868e580ba41952023-11-20T02:40:21ZengMDPI AGFoods2304-81582020-06-019672910.3390/foods9060729Effect of Instant Controlled Pressure Drop (DIC) Treatment on the Detection of Nut Allergens by Real Time PCRAfrica Sanchiz0Carmen Cuadrado1Joseph Haddad2Rosario Linacero3Food Technology Department, SGIT-INIA, Ctra. La Coruña Km. 7.5, 28040 Madrid, SpainFood Technology Department, SGIT-INIA, Ctra. La Coruña Km. 7.5, 28040 Madrid, SpainLaboratory Engineering Science for Environment (UMR 7356 CNRS), La Rochelle University, Avenue Michel Crepeau, 17042 La Rochelle, FranceGenetics, Physiology and Microbiology Department, Biology Faculty, Complutense University, 28040 Madrid, SpainTree nuts show nutritional properties and human health benefits. However, they contain allergenic proteins, which make them harmful to the sensitised population. The presence of tree nuts on food labelling is mandatory and, consequently, the development of suitable analytical methodologies to detect nuts in processed foods is advisable. Real-Time PCR allowed a specific and accurate amplification of allergen sequences. Some food processing methods could induce structural and/or conformational changes in proteins by altering their allergenic capacity, as well as produce the fragmentation and/or degradation of genomic DNA. In this work, we analysed by means of Real-Time PCR, the influence of pressure and thermal processing through Instant Controlled Pressure Drop (DIC) on the detectability of hazelnut, pistachio and cashew allergens. The detection of targets in hazelnut, pistachio and cashew (Cor a 9, Pis v 1 and Ana o 1, respectively) is affected by the treatment to different extents depending on the tree nut. Results are compared to those previously obtained by our group in the analysis of different treatments on the amplificability of the same targets. Reduction in amplificability is similar to that reported for some autoclave conditions. Our assays might allow for the detection of up to 1000 mg/kg of hazelnut, pistachio and cashew flours after being submitted to DIC treatment in food matrices.https://www.mdpi.com/2304-8158/9/6/729real-time PCRtree nutsallergen detectionprocessed foodsthermal processingpressure processing |
| spellingShingle | Africa Sanchiz Carmen Cuadrado Joseph Haddad Rosario Linacero Effect of Instant Controlled Pressure Drop (DIC) Treatment on the Detection of Nut Allergens by Real Time PCR Foods real-time PCR tree nuts allergen detection processed foods thermal processing pressure processing |
| title | Effect of Instant Controlled Pressure Drop (DIC) Treatment on the Detection of Nut Allergens by Real Time PCR |
| title_full | Effect of Instant Controlled Pressure Drop (DIC) Treatment on the Detection of Nut Allergens by Real Time PCR |
| title_fullStr | Effect of Instant Controlled Pressure Drop (DIC) Treatment on the Detection of Nut Allergens by Real Time PCR |
| title_full_unstemmed | Effect of Instant Controlled Pressure Drop (DIC) Treatment on the Detection of Nut Allergens by Real Time PCR |
| title_short | Effect of Instant Controlled Pressure Drop (DIC) Treatment on the Detection of Nut Allergens by Real Time PCR |
| title_sort | effect of instant controlled pressure drop dic treatment on the detection of nut allergens by real time pcr |
| topic | real-time PCR tree nuts allergen detection processed foods thermal processing pressure processing |
| url | https://www.mdpi.com/2304-8158/9/6/729 |
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