| Summary: | <i>Peribacillus</i> sp. AS_2, a leaf endophytic bacterium isolated from the medicinal plant <i>Alectra sessiliflora</i>, was previously identified using the 16S rRNA gene sequence. The draft genome of AS_2 had a 5,482,853 bp draft circular chromosome, 43 contigs, N<sub>50</sub> of 360,633 bp and an average G + C% content of 40.5%. Whole genome sequencing and phenotypic analysis showed that AS_2 was Gram-positive, endospore-forming, motile, and rod-shaped and it showed a high sequence similarity with <i>P. frigoritolerans</i> DSM 8801<sup>T</sup>. Genomic digital DNA–DNA hybridisation (dDDH) between strain AS_2 and <i>Peribacillus frigoritolerans</i> DSM 8801<sup>T</sup> and <i>P. castrilensis</i> N3<sup>T</sup> was 84.8% and 79.2%, respectively, and the average nucleotide identity (ANI) of strain AS_2 with <i>P. frigoritolerans</i> DSM 8801<sup>T</sup> and <i>P. castrilensis</i> N3<sup>T</sup> was 97.0% and 96.7%, respectively. The antiSMASH software predicted a total of eight secondary metabolite gene clusters comprising non-ribosomal peptide synthetase (NRPS) type koranimine, terpenes, and siderophore clusters. Strain AS_2 also displayed genes involved in endophytic lifestyle and antibiotic resistance gene clusters such as small multidrug resistance antibiotic efflux pumps (<i>qacJs</i>). Using the multilocus sequence analysis (MLSA), together with the phenotypic data and genomic analysis, we demonstrated that strain AS_2 is a subspecies of <i>P. frigoritolerans</i> DSM 8801<sup>T</sup>. Genome sequencing of <i>Peribacillus</i> sp. AS_2 from medicinal plants provides valuable genomic information and allows us to further explore its biotechnological applications.
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