Habenula GPR139 is associated with fear learning in the zebrafish
Abstract G-protein coupled receptor 139 (GPR139) is an evolutionarily conserved orphan receptor, predominantly expressing in the habenula of vertebrate species. The habenula has recently been implicated in aversive response and its associated learning. Here, we tested the hypothesis that GPR139 sign...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-85002-1 |
| _version_ | 1818345447643676672 |
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| author | Nisa Roy Satoshi Ogawa Roshan Maniam Ishwar Parhar |
| author_facet | Nisa Roy Satoshi Ogawa Roshan Maniam Ishwar Parhar |
| author_sort | Nisa Roy |
| collection | DOAJ |
| description | Abstract G-protein coupled receptor 139 (GPR139) is an evolutionarily conserved orphan receptor, predominantly expressing in the habenula of vertebrate species. The habenula has recently been implicated in aversive response and its associated learning. Here, we tested the hypothesis that GPR139 signalling in the habenula may play a role in fear learning in the zebrafish. We examined the effect of intraperitoneal injections of a human GPR139-selective agonist (JNJ-63533054) on alarm substance-induced fear learning using conditioned place avoidance paradigm, where an aversive stimulus is paired with one compartment, while its absence is associated with the other compartment of the apparatus. The results indicate that fish treated with 1 µg/g body weight of GPR139 agonist displayed no difference in locomotor activity and alarm substance-induced fear response. However, avoidance to fear-conditioned compartment was diminished, which suggests that the agonist blocks the consolidation of contextual fear memory. On the other hand, fish treated with 0.1 µg/g body weight of GPR139 agonist spent a significantly longer time in the unconditioned neutral compartment as compared to the conditioned (punished and unpunished) compartments. These results suggest that activation of GPR139 signalling in the habenula may be involved in fear learning and the decision-making process in the zebrafish. |
| first_indexed | 2024-12-13T17:02:31Z |
| format | Article |
| id | doaj.art-941f840711fb4d1a8f142f93f267a263 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-13T17:02:31Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-941f840711fb4d1a8f142f93f267a2632022-12-21T23:37:47ZengNature PortfolioScientific Reports2045-23222021-03-0111111410.1038/s41598-021-85002-1Habenula GPR139 is associated with fear learning in the zebrafishNisa Roy0Satoshi Ogawa1Roshan Maniam2Ishwar Parhar3Brain Research Institute, Jeffrey Cheah School of Medicine and Health Sciences, Monash University MalaysiaBrain Research Institute, Jeffrey Cheah School of Medicine and Health Sciences, Monash University MalaysiaBrain Research Institute, Jeffrey Cheah School of Medicine and Health Sciences, Monash University MalaysiaBrain Research Institute, Jeffrey Cheah School of Medicine and Health Sciences, Monash University MalaysiaAbstract G-protein coupled receptor 139 (GPR139) is an evolutionarily conserved orphan receptor, predominantly expressing in the habenula of vertebrate species. The habenula has recently been implicated in aversive response and its associated learning. Here, we tested the hypothesis that GPR139 signalling in the habenula may play a role in fear learning in the zebrafish. We examined the effect of intraperitoneal injections of a human GPR139-selective agonist (JNJ-63533054) on alarm substance-induced fear learning using conditioned place avoidance paradigm, where an aversive stimulus is paired with one compartment, while its absence is associated with the other compartment of the apparatus. The results indicate that fish treated with 1 µg/g body weight of GPR139 agonist displayed no difference in locomotor activity and alarm substance-induced fear response. However, avoidance to fear-conditioned compartment was diminished, which suggests that the agonist blocks the consolidation of contextual fear memory. On the other hand, fish treated with 0.1 µg/g body weight of GPR139 agonist spent a significantly longer time in the unconditioned neutral compartment as compared to the conditioned (punished and unpunished) compartments. These results suggest that activation of GPR139 signalling in the habenula may be involved in fear learning and the decision-making process in the zebrafish.https://doi.org/10.1038/s41598-021-85002-1 |
| spellingShingle | Nisa Roy Satoshi Ogawa Roshan Maniam Ishwar Parhar Habenula GPR139 is associated with fear learning in the zebrafish Scientific Reports |
| title | Habenula GPR139 is associated with fear learning in the zebrafish |
| title_full | Habenula GPR139 is associated with fear learning in the zebrafish |
| title_fullStr | Habenula GPR139 is associated with fear learning in the zebrafish |
| title_full_unstemmed | Habenula GPR139 is associated with fear learning in the zebrafish |
| title_short | Habenula GPR139 is associated with fear learning in the zebrafish |
| title_sort | habenula gpr139 is associated with fear learning in the zebrafish |
| url | https://doi.org/10.1038/s41598-021-85002-1 |
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