Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck
Intraoperative definition of tumor free resection margins in head and neck cancer is challenging. In the current proof-of-principle study we evaluated a novel silica nanoparticle-based agent for its potential use as contrast enhancer. We synthesized silica nanoparticles with an average size of 45 nm...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/9/10/1378 |
| _version_ | 1818959452484665344 |
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| author | Anna Watermann Rita Gieringer Anna-Maria Bauer Sven Kurch Ralf Kiesslich Wolfgang Tremel Jan Gosepath Juergen Brieger |
| author_facet | Anna Watermann Rita Gieringer Anna-Maria Bauer Sven Kurch Ralf Kiesslich Wolfgang Tremel Jan Gosepath Juergen Brieger |
| author_sort | Anna Watermann |
| collection | DOAJ |
| description | Intraoperative definition of tumor free resection margins in head and neck cancer is challenging. In the current proof-of-principle study we evaluated a novel silica nanoparticle-based agent for its potential use as contrast enhancer. We synthesized silica nanoparticles with an average size of 45 nm and modified these particles with the fluorescence stain fluorescein isocyanate (FITC) for particle detection and with epidermal growth factor receptor (EGFR)-targeting antibodies for enhanced tumor specificity. The nanoparticles exhibited good biocompatibility and could be detected in vitro and in vivo by confocal laser scanning microscopy. Additionally, we show in an ex vivo setting that these modified nanoparticles specifically bind to tumor samples and could be detected using a handheld confocal fluorescence endomicroscope. From a clinical point of view, we believe that this method could be used for tumor border contrast enhancement and for better intraoperative definition of R-0 tumor resection. |
| first_indexed | 2024-12-20T11:41:52Z |
| format | Article |
| id | doaj.art-942025c5987c4eae94987f666a39c8d7 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-20T11:41:52Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-942025c5987c4eae94987f666a39c8d72022-12-21T19:41:58ZengMDPI AGNanomaterials2079-49912019-09-01910137810.3390/nano9101378nano9101378Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and NeckAnna Watermann0Rita Gieringer1Anna-Maria Bauer2Sven Kurch3Ralf Kiesslich4Wolfgang Tremel5Jan Gosepath6Juergen Brieger7Molecular Tumorbiology, Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University Mainz, 55131 Mainz, GermanyMolecular Tumorbiology, Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University Mainz, 55131 Mainz, GermanyInstitute of Inorganic Chemistry and Analytical Chemistry, Johannes Gutenberg University, 55128 Mainz, GermanyInstitute of Inorganic Chemistry and Analytical Chemistry, Johannes Gutenberg University, 55128 Mainz, GermanyHSK Klinik für Innere Medizin II: Gastroenterologie, Hepatologie und Endokrinologie, 65199 Wiesbaden, GermanyInstitute of Inorganic Chemistry and Analytical Chemistry, Johannes Gutenberg University, 55128 Mainz, GermanyHSK Klinik für Hals-, Nasen- und Ohrenheilkunde, Kopf- und Hals-Chirugie, 65199 Wiesbaden, GermanyMolecular Tumorbiology, Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University Mainz, 55131 Mainz, GermanyIntraoperative definition of tumor free resection margins in head and neck cancer is challenging. In the current proof-of-principle study we evaluated a novel silica nanoparticle-based agent for its potential use as contrast enhancer. We synthesized silica nanoparticles with an average size of 45 nm and modified these particles with the fluorescence stain fluorescein isocyanate (FITC) for particle detection and with epidermal growth factor receptor (EGFR)-targeting antibodies for enhanced tumor specificity. The nanoparticles exhibited good biocompatibility and could be detected in vitro and in vivo by confocal laser scanning microscopy. Additionally, we show in an ex vivo setting that these modified nanoparticles specifically bind to tumor samples and could be detected using a handheld confocal fluorescence endomicroscope. From a clinical point of view, we believe that this method could be used for tumor border contrast enhancement and for better intraoperative definition of R-0 tumor resection.https://www.mdpi.com/2079-4991/9/10/1378contrast agentsilica nanoparticlesegfr |
| spellingShingle | Anna Watermann Rita Gieringer Anna-Maria Bauer Sven Kurch Ralf Kiesslich Wolfgang Tremel Jan Gosepath Juergen Brieger Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck Nanomaterials contrast agent silica nanoparticles egfr |
| title | Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck |
| title_full | Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck |
| title_fullStr | Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck |
| title_full_unstemmed | Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck |
| title_short | Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck |
| title_sort | fluorescein and egfr antibody conjugated silica nanoparticles for enhancement of real time tumor border definition using confocal laser endomicroscopy in squamous cell carcinoma of the head and neck |
| topic | contrast agent silica nanoparticles egfr |
| url | https://www.mdpi.com/2079-4991/9/10/1378 |
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