Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and Prospects
The cell-based approach in gene therapy arises as a promising strategy to provide safe, targeted, and efficient gene delivery. Owing to their unique features, as homing and tumor-tropism, mesenchymal stem cells (MSCs) have recently been introduced as an encouraging vehicle in gene therapy. Neverthel...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/13/6/843 |
| _version_ | 1797531024304898048 |
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| author | Noha Attia Mohamed Mashal Gustavo Puras Jose Luis Pedraz |
| author_facet | Noha Attia Mohamed Mashal Gustavo Puras Jose Luis Pedraz |
| author_sort | Noha Attia |
| collection | DOAJ |
| description | The cell-based approach in gene therapy arises as a promising strategy to provide safe, targeted, and efficient gene delivery. Owing to their unique features, as homing and tumor-tropism, mesenchymal stem cells (MSCs) have recently been introduced as an encouraging vehicle in gene therapy. Nevertheless, non-viral transfer of nucleic acids into MSCs remains limited due to various factors related to the main stakeholders of the process (e.g., nucleic acids, carriers, or cells). In this review, we have summarized the main types of nucleic acids used to transfect MSCs, the pros and cons, and applications of each. Then, we have emphasized on the most efficient lipid-based carriers for nucleic acids to MSCs, their main features, and some of their applications. While a myriad of studies have demonstrated the therapeutic potential for engineered MSCs therapy in various illnesses, optimization for clinical use is an ongoing challenge. On the way of improvement, genetically modified MSCs have been combined with various novel techniques and tools (e.g., exosomes, spheroids, 3D-Bioprinting, etc.,) aiming for more efficient and safe applications in biomedicine. |
| first_indexed | 2024-03-10T10:38:13Z |
| format | Article |
| id | doaj.art-9421de832f74403d8f81eaa7a96d1b7c |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T10:38:13Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-9421de832f74403d8f81eaa7a96d1b7c2023-11-21T23:08:45ZengMDPI AGPharmaceutics1999-49232021-06-0113684310.3390/pharmaceutics13060843Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and ProspectsNoha Attia0Mohamed Mashal1Gustavo Puras2Jose Luis Pedraz3Laboratory of Pharmaceutics, NanoBioCel Research Group, School of Pharmacy, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, SpainLaboratory of Pharmaceutics, NanoBioCel Research Group, School of Pharmacy, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, SpainLaboratory of Pharmaceutics, NanoBioCel Research Group, School of Pharmacy, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, SpainLaboratory of Pharmaceutics, NanoBioCel Research Group, School of Pharmacy, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, SpainThe cell-based approach in gene therapy arises as a promising strategy to provide safe, targeted, and efficient gene delivery. Owing to their unique features, as homing and tumor-tropism, mesenchymal stem cells (MSCs) have recently been introduced as an encouraging vehicle in gene therapy. Nevertheless, non-viral transfer of nucleic acids into MSCs remains limited due to various factors related to the main stakeholders of the process (e.g., nucleic acids, carriers, or cells). In this review, we have summarized the main types of nucleic acids used to transfect MSCs, the pros and cons, and applications of each. Then, we have emphasized on the most efficient lipid-based carriers for nucleic acids to MSCs, their main features, and some of their applications. While a myriad of studies have demonstrated the therapeutic potential for engineered MSCs therapy in various illnesses, optimization for clinical use is an ongoing challenge. On the way of improvement, genetically modified MSCs have been combined with various novel techniques and tools (e.g., exosomes, spheroids, 3D-Bioprinting, etc.,) aiming for more efficient and safe applications in biomedicine.https://www.mdpi.com/1999-4923/13/6/843non-viral gene delivery3D-bioprintingtransfectionmesenchymal stem cellcell therapygene therapy |
| spellingShingle | Noha Attia Mohamed Mashal Gustavo Puras Jose Luis Pedraz Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and Prospects Pharmaceutics non-viral gene delivery 3D-bioprinting transfection mesenchymal stem cell cell therapy gene therapy |
| title | Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and Prospects |
| title_full | Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and Prospects |
| title_fullStr | Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and Prospects |
| title_full_unstemmed | Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and Prospects |
| title_short | Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and Prospects |
| title_sort | mesenchymal stem cells as a gene delivery tool promise problems and prospects |
| topic | non-viral gene delivery 3D-bioprinting transfection mesenchymal stem cell cell therapy gene therapy |
| url | https://www.mdpi.com/1999-4923/13/6/843 |
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