3D-Printed Collagen-Based Waveform Microfibrous Scaffold for Periodontal Ligament Reconstruction
Reconstruction of the periodontal ligament (PDL) to fulfill functional requirement remains a challenge. This study sought to develop a biomimetic microfibrous system capable of withstanding the functional load to assist PDL regeneration. Collagen-based straight and waveform microfibers to guide PDL...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/14/7725 |
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| author | Hsu-Hsiang Lin Pen-Hsiu Grace Chao Wei-Chiu Tai Po-Chun Chang |
| author_facet | Hsu-Hsiang Lin Pen-Hsiu Grace Chao Wei-Chiu Tai Po-Chun Chang |
| author_sort | Hsu-Hsiang Lin |
| collection | DOAJ |
| description | Reconstruction of the periodontal ligament (PDL) to fulfill functional requirement remains a challenge. This study sought to develop a biomimetic microfibrous system capable of withstanding the functional load to assist PDL regeneration. Collagen-based straight and waveform microfibers to guide PDL cell growth were prepared using an extrusion-based bioprinter, and a laminar flow-based bioreactor was used to generate fluidic shear stress. PDL cells were seeded on the respective microfibers with 0 or 6 dynes/cm<sup>2</sup> fluidic shear stress for 1–4 h. The viability, morphology, adhesion pattern, and gene expression levels of PDL cells were assessed. The results revealed that upon bioprinting optimization, collagen-based microfibers were successfully fabricated. The straight microfibers were 189.9 ± 11.44 μm wide and the waveform microfibers were 235.9 ± 11.22 μm wide. Under 6 dynes/cm<sup>2</sup> shear stress, PDL cells were successfully seeded, and cytoskeleton expansion, adhesion, and viability were greater. Cyclin D, E-cadherin, and periostin were upregulated on the waveform microfibers. In conclusion, 3D-printed collagen-based waveform microfibers preserved PDL cell viability and exhibited an enhanced tendency to promote healing and regeneration under shear stress. This approach is promising for the development of a guiding scaffold for PDL regeneration. |
| first_indexed | 2024-03-10T09:36:27Z |
| format | Article |
| id | doaj.art-942572f9d9634fda89dcf3b543b2794d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T09:36:27Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-942572f9d9634fda89dcf3b543b2794d2023-11-22T04:03:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-012214772510.3390/ijms221477253D-Printed Collagen-Based Waveform Microfibrous Scaffold for Periodontal Ligament ReconstructionHsu-Hsiang Lin0Pen-Hsiu Grace Chao1Wei-Chiu Tai2Po-Chun Chang3Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei 10048, TaiwanDepartment of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 10617, TaiwanGraduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei 10048, TaiwanGraduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei 10048, TaiwanReconstruction of the periodontal ligament (PDL) to fulfill functional requirement remains a challenge. This study sought to develop a biomimetic microfibrous system capable of withstanding the functional load to assist PDL regeneration. Collagen-based straight and waveform microfibers to guide PDL cell growth were prepared using an extrusion-based bioprinter, and a laminar flow-based bioreactor was used to generate fluidic shear stress. PDL cells were seeded on the respective microfibers with 0 or 6 dynes/cm<sup>2</sup> fluidic shear stress for 1–4 h. The viability, morphology, adhesion pattern, and gene expression levels of PDL cells were assessed. The results revealed that upon bioprinting optimization, collagen-based microfibers were successfully fabricated. The straight microfibers were 189.9 ± 11.44 μm wide and the waveform microfibers were 235.9 ± 11.22 μm wide. Under 6 dynes/cm<sup>2</sup> shear stress, PDL cells were successfully seeded, and cytoskeleton expansion, adhesion, and viability were greater. Cyclin D, E-cadherin, and periostin were upregulated on the waveform microfibers. In conclusion, 3D-printed collagen-based waveform microfibers preserved PDL cell viability and exhibited an enhanced tendency to promote healing and regeneration under shear stress. This approach is promising for the development of a guiding scaffold for PDL regeneration.https://www.mdpi.com/1422-0067/22/14/7725periodontal ligamenttissue engineeringbioprintingcollagen |
| spellingShingle | Hsu-Hsiang Lin Pen-Hsiu Grace Chao Wei-Chiu Tai Po-Chun Chang 3D-Printed Collagen-Based Waveform Microfibrous Scaffold for Periodontal Ligament Reconstruction International Journal of Molecular Sciences periodontal ligament tissue engineering bioprinting collagen |
| title | 3D-Printed Collagen-Based Waveform Microfibrous Scaffold for Periodontal Ligament Reconstruction |
| title_full | 3D-Printed Collagen-Based Waveform Microfibrous Scaffold for Periodontal Ligament Reconstruction |
| title_fullStr | 3D-Printed Collagen-Based Waveform Microfibrous Scaffold for Periodontal Ligament Reconstruction |
| title_full_unstemmed | 3D-Printed Collagen-Based Waveform Microfibrous Scaffold for Periodontal Ligament Reconstruction |
| title_short | 3D-Printed Collagen-Based Waveform Microfibrous Scaffold for Periodontal Ligament Reconstruction |
| title_sort | 3d printed collagen based waveform microfibrous scaffold for periodontal ligament reconstruction |
| topic | periodontal ligament tissue engineering bioprinting collagen |
| url | https://www.mdpi.com/1422-0067/22/14/7725 |
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