Antiadipogenic Effects of Mixtures of <i>Cornus officinalis</i> and <i>Ribes fasciculatum</i> Extracts on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Mice
Medicinal plants have been used worldwide as primary alternative healthcare supplements. <i>Cornus officinalis</i> (CO) and <i>Ribes fasciculatum</i> (RF) are traditional medicinal plants applied in East Asia to treat human diseases such as hepatitis, osteoporosis, oxidative...
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MDPI AG
2020-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/10/2350 |
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| author | Eunkuk Park Chang Gun Lee Hyesoo Jeong Subin Yeo Ji Ae Kim Seon-Yong Jeong |
| author_facet | Eunkuk Park Chang Gun Lee Hyesoo Jeong Subin Yeo Ji Ae Kim Seon-Yong Jeong |
| author_sort | Eunkuk Park |
| collection | DOAJ |
| description | Medicinal plants have been used worldwide as primary alternative healthcare supplements. <i>Cornus officinalis</i> (CO) and <i>Ribes fasciculatum</i> (RF) are traditional medicinal plants applied in East Asia to treat human diseases such as hepatitis, osteoporosis, oxidative stress and allergy. The aim of this study was to examine the anti-obesity effect of CO and RF on preadipocyte 3T3-L1 cells in vitro and high-fat diet (HFD)-induced obesity mice in vivo. Combination treatment of CO and RF in differentiated 3T3-L1 cells inhibited adipocyte differentiation through downregulation of adipogenesis-associated genes such as CCAAT/enhancer-binding protein alpha (<i>Cebpa</i>), fatty acid binding protein 4 (<i>Fabp4</i>), peroxisome proliferator-activated receptor gamma <i>(Pparg</i>) and sterol regulatory element binding protein (<i>Srebp1</i>). In vivo animal models showed that a mixture of CO and RF inhibited HFD-induced weight gain, resulting in decreased abdominal visceral fat tissues and fatty hepatocyte deposition. In addition, CO+RF treatment decreased HFD-induced adipogenesis-associated genes in abdominal white fat tissue. These results suggest that administration of a CO and RF mixture prevented adipocyte differentiation and lipid accumulation in preadipocyte cells and HFD-induced body weight in obesity mice. Therefore, combined therapy of CO and RF may be a protective therapeutic agent against obesity. |
| first_indexed | 2024-03-10T19:45:53Z |
| format | Article |
| id | doaj.art-942b945c9e6c4c53b913779a67c5ad44 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T19:45:53Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-942b945c9e6c4c53b913779a67c5ad442023-11-20T00:49:54ZengMDPI AGMolecules1420-30492020-05-012510235010.3390/molecules25102350Antiadipogenic Effects of Mixtures of <i>Cornus officinalis</i> and <i>Ribes fasciculatum</i> Extracts on 3T3-L1 Preadipocytes and High-Fat Diet-Induced MiceEunkuk Park0Chang Gun Lee1Hyesoo Jeong2Subin Yeo3Ji Ae Kim4Seon-Yong Jeong5Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, KoreaDepartment of Medical Genetics, Ajou University School of Medicine, Suwon 16499, KoreaNine B Company, Daejeon 34121, KoreaNine B Company, Daejeon 34121, KoreaNine B Company, Daejeon 34121, KoreaDepartment of Medical Genetics, Ajou University School of Medicine, Suwon 16499, KoreaMedicinal plants have been used worldwide as primary alternative healthcare supplements. <i>Cornus officinalis</i> (CO) and <i>Ribes fasciculatum</i> (RF) are traditional medicinal plants applied in East Asia to treat human diseases such as hepatitis, osteoporosis, oxidative stress and allergy. The aim of this study was to examine the anti-obesity effect of CO and RF on preadipocyte 3T3-L1 cells in vitro and high-fat diet (HFD)-induced obesity mice in vivo. Combination treatment of CO and RF in differentiated 3T3-L1 cells inhibited adipocyte differentiation through downregulation of adipogenesis-associated genes such as CCAAT/enhancer-binding protein alpha (<i>Cebpa</i>), fatty acid binding protein 4 (<i>Fabp4</i>), peroxisome proliferator-activated receptor gamma <i>(Pparg</i>) and sterol regulatory element binding protein (<i>Srebp1</i>). In vivo animal models showed that a mixture of CO and RF inhibited HFD-induced weight gain, resulting in decreased abdominal visceral fat tissues and fatty hepatocyte deposition. In addition, CO+RF treatment decreased HFD-induced adipogenesis-associated genes in abdominal white fat tissue. These results suggest that administration of a CO and RF mixture prevented adipocyte differentiation and lipid accumulation in preadipocyte cells and HFD-induced body weight in obesity mice. Therefore, combined therapy of CO and RF may be a protective therapeutic agent against obesity.https://www.mdpi.com/1420-3049/25/10/2350obesity<i>Cornus</i> <i>officinalis</i><i>Ribes</i> <i>fasciculatum</i>high-fat dietnatural product |
| spellingShingle | Eunkuk Park Chang Gun Lee Hyesoo Jeong Subin Yeo Ji Ae Kim Seon-Yong Jeong Antiadipogenic Effects of Mixtures of <i>Cornus officinalis</i> and <i>Ribes fasciculatum</i> Extracts on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Mice Molecules obesity <i>Cornus</i> <i>officinalis</i> <i>Ribes</i> <i>fasciculatum</i> high-fat diet natural product |
| title | Antiadipogenic Effects of Mixtures of <i>Cornus officinalis</i> and <i>Ribes fasciculatum</i> Extracts on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Mice |
| title_full | Antiadipogenic Effects of Mixtures of <i>Cornus officinalis</i> and <i>Ribes fasciculatum</i> Extracts on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Mice |
| title_fullStr | Antiadipogenic Effects of Mixtures of <i>Cornus officinalis</i> and <i>Ribes fasciculatum</i> Extracts on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Mice |
| title_full_unstemmed | Antiadipogenic Effects of Mixtures of <i>Cornus officinalis</i> and <i>Ribes fasciculatum</i> Extracts on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Mice |
| title_short | Antiadipogenic Effects of Mixtures of <i>Cornus officinalis</i> and <i>Ribes fasciculatum</i> Extracts on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Mice |
| title_sort | antiadipogenic effects of mixtures of i cornus officinalis i and i ribes fasciculatum i extracts on 3t3 l1 preadipocytes and high fat diet induced mice |
| topic | obesity <i>Cornus</i> <i>officinalis</i> <i>Ribes</i> <i>fasciculatum</i> high-fat diet natural product |
| url | https://www.mdpi.com/1420-3049/25/10/2350 |
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