Development of a quantum dot-based lateral flow immunoassay strip for rapid and sensitive detection of SARS-CoV-2 neutralizing antibodies
Abstract To a certain extent, the development and vaccination of COVID-19 vaccine have reduced the alarming rate of transmission speed and mortality rate. At present, vaccine coverage is quite high in countries around the world. Since individual differences are unavoidable, it is necessary to assess...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-49244-5 |
| _version_ | 1797388356509761536 |
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| author | Xirong Wang Shulin Shao Huan Ye Sen Li Bing Gu Bo Tang |
| author_facet | Xirong Wang Shulin Shao Huan Ye Sen Li Bing Gu Bo Tang |
| author_sort | Xirong Wang |
| collection | DOAJ |
| description | Abstract To a certain extent, the development and vaccination of COVID-19 vaccine have reduced the alarming rate of transmission speed and mortality rate. At present, vaccine coverage is quite high in countries around the world. Since individual differences are unavoidable, it is necessary to assess the efficacy of the vaccine in each vaccinated person in order to reflect the protective effect of the vaccine in different populations. In this study, we developed a novel COVID-19 neutralizing antibody detection kit combining lateral flow immunochromatography and novel quantum dot technology with 85.23% sensitivity, 92.50% specificity. The novel QD-ICA could achieve an accurate detection of SARS-CoV-2 neutralizing antibodies with 10 minutes, two steps, small equipment size, and broad testing application, suggesting its capability to assess vaccine effectiveness on a large scale in areas of world that currently affected by the pandemic. |
| first_indexed | 2024-03-08T22:38:33Z |
| format | Article |
| id | doaj.art-942c95f63eac4cd3b99a3ad46eb03392 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T22:38:33Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-942c95f63eac4cd3b99a3ad46eb033922023-12-17T12:17:31ZengNature PortfolioScientific Reports2045-23222023-12-011311810.1038/s41598-023-49244-5Development of a quantum dot-based lateral flow immunoassay strip for rapid and sensitive detection of SARS-CoV-2 neutralizing antibodiesXirong Wang0Shulin Shao1Huan Ye2Sen Li3Bing Gu4Bo Tang5Medical Technology School of Xuzhou Medical UniversityDepartment of Laboratory, Nanjing Pukou Hospital of Traditional Chinese MedicineNanjing Vazyme Medical Technology Co. Ltd.Nanjing Vazyme Medical Technology Co. Ltd.Laboratory Medicine, Guangdong Provincial People’s HospitalMedical Technology School of Xuzhou Medical UniversityAbstract To a certain extent, the development and vaccination of COVID-19 vaccine have reduced the alarming rate of transmission speed and mortality rate. At present, vaccine coverage is quite high in countries around the world. Since individual differences are unavoidable, it is necessary to assess the efficacy of the vaccine in each vaccinated person in order to reflect the protective effect of the vaccine in different populations. In this study, we developed a novel COVID-19 neutralizing antibody detection kit combining lateral flow immunochromatography and novel quantum dot technology with 85.23% sensitivity, 92.50% specificity. The novel QD-ICA could achieve an accurate detection of SARS-CoV-2 neutralizing antibodies with 10 minutes, two steps, small equipment size, and broad testing application, suggesting its capability to assess vaccine effectiveness on a large scale in areas of world that currently affected by the pandemic.https://doi.org/10.1038/s41598-023-49244-5 |
| spellingShingle | Xirong Wang Shulin Shao Huan Ye Sen Li Bing Gu Bo Tang Development of a quantum dot-based lateral flow immunoassay strip for rapid and sensitive detection of SARS-CoV-2 neutralizing antibodies Scientific Reports |
| title | Development of a quantum dot-based lateral flow immunoassay strip for rapid and sensitive detection of SARS-CoV-2 neutralizing antibodies |
| title_full | Development of a quantum dot-based lateral flow immunoassay strip for rapid and sensitive detection of SARS-CoV-2 neutralizing antibodies |
| title_fullStr | Development of a quantum dot-based lateral flow immunoassay strip for rapid and sensitive detection of SARS-CoV-2 neutralizing antibodies |
| title_full_unstemmed | Development of a quantum dot-based lateral flow immunoassay strip for rapid and sensitive detection of SARS-CoV-2 neutralizing antibodies |
| title_short | Development of a quantum dot-based lateral flow immunoassay strip for rapid and sensitive detection of SARS-CoV-2 neutralizing antibodies |
| title_sort | development of a quantum dot based lateral flow immunoassay strip for rapid and sensitive detection of sars cov 2 neutralizing antibodies |
| url | https://doi.org/10.1038/s41598-023-49244-5 |
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