A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells
Zhi-Qiang Zhang, Wen-Jing Yao, Lu-Lu Qiao, Xiaojing Yang, Jiahua Shi, Mei-Xia Zhao Key Laboratory of Natural Medicine and Immune Engineering, Henan University, Kaifeng 475004, People’s Republic of ChinaCorrespondence: Mei-Xia ZhaoKey Laboratory of Natural Medicine and Immune Engineering, H...
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| Format: | Article |
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Dove Medical Press
2020-03-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/a-lysosome-targetable-fluorescence-probe-based-on-l-cysteine-polyamine-peer-reviewed-article-IJN |
| _version_ | 1818401355332583424 |
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| author | Zhang ZQ Yao WJ Qiao LL Yang X Shi J Zhao MX |
| author_facet | Zhang ZQ Yao WJ Qiao LL Yang X Shi J Zhao MX |
| author_sort | Zhang ZQ |
| collection | DOAJ |
| description | Zhi-Qiang Zhang, Wen-Jing Yao, Lu-Lu Qiao, Xiaojing Yang, Jiahua Shi, Mei-Xia Zhao Key Laboratory of Natural Medicine and Immune Engineering, Henan University, Kaifeng 475004, People’s Republic of ChinaCorrespondence: Mei-Xia ZhaoKey Laboratory of Natural Medicine and Immune Engineering, Henan University, Jinming Road, Kaifeng 475004, People’s Republic of ChinaEmail zhaomeixia2011@henu.edu.cnPurpose: Quantum dots (QDs) are used as fluorescent probes due to their high fluorescence intensity, longevity of fluorescence, strong light-resistant bleaching ability and high light stability. Therefore, we explore a more precise probe that can target an organelle.Methods: In the current study, a new class of fluorescence probes were developed using QDs capped with 4 different L-cysteine-polyamine-morpholine linked by mercapto groups. Ligands were characterised by Electrospray ionization mass spectrometry (ESI-MS), H-Nuclear Magnetic Resonance (1H NMR) spectroscopy, and 13C NMR spectroscopy. Modified QDs were characterized by Transmission Electron Microscope (TEM), Ultraviolet and visible spectrophotometry (UV–Vis), and fluorescence microscopy. And the biological activity of modified QDs was explored by using MTT assay with HeLa, SMMC-7721 and HepG2 cells. The fluorescence imaging of modified QDs was obtained by confocal laser scanning fluorescence microscopy (CLSM).Results: Synthesized QDs ranged between 4 to 5 nm and had strong optical emission properties. UV–Vis and fluorescence spectra demonstrated that the cysteine-polyamine-morpholine were successfully incorporated into QD nanoparticles. The MTT results demonstrated that modified QDs had lesser cytotoxicity when compared to unmodified QDs. In addition, modified QDs had strong fluorescence intensity in HeLa cells and targeted lysosomes of HeLa cells.Conclusion: This study demonstrates the modified QDs efficiently entered cells and could be used as a potential lysosome-targeting fluorescent probe.Keywords: quantum dots, fluorescence probe, lysosome-targetable, cells imaging |
| first_indexed | 2024-12-14T07:51:09Z |
| format | Article |
| id | doaj.art-942ca50ed3774677b4b1b38f015f397e |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-14T07:51:09Z |
| publishDate | 2020-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-942ca50ed3774677b4b1b38f015f397e2022-12-21T23:10:43ZengDove Medical PressInternational Journal of Nanomedicine1178-20132020-03-01Volume 151611162252372A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living CellsZhang ZQYao WJQiao LLYang XShi JZhao MXZhi-Qiang Zhang, Wen-Jing Yao, Lu-Lu Qiao, Xiaojing Yang, Jiahua Shi, Mei-Xia Zhao Key Laboratory of Natural Medicine and Immune Engineering, Henan University, Kaifeng 475004, People’s Republic of ChinaCorrespondence: Mei-Xia ZhaoKey Laboratory of Natural Medicine and Immune Engineering, Henan University, Jinming Road, Kaifeng 475004, People’s Republic of ChinaEmail zhaomeixia2011@henu.edu.cnPurpose: Quantum dots (QDs) are used as fluorescent probes due to their high fluorescence intensity, longevity of fluorescence, strong light-resistant bleaching ability and high light stability. Therefore, we explore a more precise probe that can target an organelle.Methods: In the current study, a new class of fluorescence probes were developed using QDs capped with 4 different L-cysteine-polyamine-morpholine linked by mercapto groups. Ligands were characterised by Electrospray ionization mass spectrometry (ESI-MS), H-Nuclear Magnetic Resonance (1H NMR) spectroscopy, and 13C NMR spectroscopy. Modified QDs were characterized by Transmission Electron Microscope (TEM), Ultraviolet and visible spectrophotometry (UV–Vis), and fluorescence microscopy. And the biological activity of modified QDs was explored by using MTT assay with HeLa, SMMC-7721 and HepG2 cells. The fluorescence imaging of modified QDs was obtained by confocal laser scanning fluorescence microscopy (CLSM).Results: Synthesized QDs ranged between 4 to 5 nm and had strong optical emission properties. UV–Vis and fluorescence spectra demonstrated that the cysteine-polyamine-morpholine were successfully incorporated into QD nanoparticles. The MTT results demonstrated that modified QDs had lesser cytotoxicity when compared to unmodified QDs. In addition, modified QDs had strong fluorescence intensity in HeLa cells and targeted lysosomes of HeLa cells.Conclusion: This study demonstrates the modified QDs efficiently entered cells and could be used as a potential lysosome-targeting fluorescent probe.Keywords: quantum dots, fluorescence probe, lysosome-targetable, cells imaginghttps://www.dovepress.com/a-lysosome-targetable-fluorescence-probe-based-on-l-cysteine-polyamine-peer-reviewed-article-IJNquantum dotsfluorescence probelysosome-targetablecells imaging |
| spellingShingle | Zhang ZQ Yao WJ Qiao LL Yang X Shi J Zhao MX A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells International Journal of Nanomedicine quantum dots fluorescence probe lysosome-targetable cells imaging |
| title | A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells |
| title_full | A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells |
| title_fullStr | A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells |
| title_full_unstemmed | A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells |
| title_short | A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells |
| title_sort | lysosome targetable fluorescence probe based on l cysteine polyamine morpholine modified quantum dots for imaging in living cells |
| topic | quantum dots fluorescence probe lysosome-targetable cells imaging |
| url | https://www.dovepress.com/a-lysosome-targetable-fluorescence-probe-based-on-l-cysteine-polyamine-peer-reviewed-article-IJN |
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