Identification of Gut Microbiota Profile Associated with Colorectal Cancer in Saudi Population
Colorectal cancer (CRC) is a significant global health concern. Microbial dysbiosis and associated metabolites have been associated with CRC occurrence and progression. This study aims to analyze the gut microbiota composition and the enriched metabolic pathways in patients with late-stage CRC. In t...
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MDPI AG
2023-10-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/20/5019 |
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| author | Areej A. Alhhazmi Yahya A. Almutawif Walaa A. Mumena Shaima M. Alhazmi Turki S. Abujamel Ruba M. Alhusayni Raghad Aloufi Razan R. Al-Hejaili Rahaf Alhujaily Lama M. Alrehaili Ruya A. Alsaedy Rahaf H. Khoja Wassal Ahmed Mohamed F. Abdelmohsen Waleed Mohammed-Saeid |
| author_facet | Areej A. Alhhazmi Yahya A. Almutawif Walaa A. Mumena Shaima M. Alhazmi Turki S. Abujamel Ruba M. Alhusayni Raghad Aloufi Razan R. Al-Hejaili Rahaf Alhujaily Lama M. Alrehaili Ruya A. Alsaedy Rahaf H. Khoja Wassal Ahmed Mohamed F. Abdelmohsen Waleed Mohammed-Saeid |
| author_sort | Areej A. Alhhazmi |
| collection | DOAJ |
| description | Colorectal cancer (CRC) is a significant global health concern. Microbial dysbiosis and associated metabolites have been associated with CRC occurrence and progression. This study aims to analyze the gut microbiota composition and the enriched metabolic pathways in patients with late-stage CRC. In this study, a cohort of 25 CRC patients diagnosed at late stage III and IV and 25 healthy participants were enrolled. The fecal bacterial composition was investigated using V3-V4 ribosomal RNA gene sequencing, followed by clustering and linear discriminant analysis (LDA) effect size (LEfSe) analyses. A cluster of ortholog genes’ (COG) functional annotations and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were employed to identify enrichment pathways between the two groups. The findings showed that the fecal microbiota between the two groups varied significantly in alpha and beta diversities. CRC patients’ fecal samples had significantly enriched populations of <i>Streptococcus salivarius</i>, <i>S. parasanguins</i>, <i>S. anginosus</i>, <i>Lactobacillus mucosae</i>, <i>L. gasseri</i>, <i>Peptostreptococcus</i>, <i>Eubacterium</i>, <i>Aerococcus</i>, Family XIII_AD3001 Group, <i>Erysipelatoclostridium</i>, <i>Escherichia-Shigella</i>, <i>Klebsiella</i>, <i>Enterobacter</i>, <i>Alistipes</i>, <i>Ralstonia</i>, and <i>Pseudomonas</i> (Q < 0.05). The enriched pathways identified in the CRC group were amino acid transport, signaling and metabolism, membrane biogenesis, DNA replication and mismatch repair system, and protease activity (Q < 0.05). These results suggested that the imbalance between intestinal bacteria and the elevated level of the predicated functions and pathways may contribute to the development of advanced CRC tumors. Further research is warranted to elucidate the exact role of the gut microbiome in CRC and its potential implications for use in diagnostic, prevention, and treatment strategies. |
| first_indexed | 2024-03-10T21:21:44Z |
| format | Article |
| id | doaj.art-942e6c7879984864b62d5ed61d7c0f03 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T21:21:44Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-942e6c7879984864b62d5ed61d7c0f032023-11-19T15:59:25ZengMDPI AGCancers2072-66942023-10-011520501910.3390/cancers15205019Identification of Gut Microbiota Profile Associated with Colorectal Cancer in Saudi PopulationAreej A. Alhhazmi0Yahya A. Almutawif1Walaa A. Mumena2Shaima M. Alhazmi3Turki S. Abujamel4Ruba M. Alhusayni5Raghad Aloufi6Razan R. Al-Hejaili7Rahaf Alhujaily8Lama M. Alrehaili9Ruya A. Alsaedy10Rahaf H. Khoja11Wassal Ahmed12Mohamed F. Abdelmohsen13Waleed Mohammed-Saeid14Medical Laboratories Technology Department, College of Applied Medical Sciences, Taibah University, Al-Madinah Al-Munawarah 42353, Saudi ArabiaMedical Laboratories Technology Department, College of Applied Medical Sciences, Taibah University, Al-Madinah Al-Munawarah 42353, Saudi ArabiaClinical Nutrition Department, College of Applied Medical Sciences, Taibah University, Al-Madinah Al-Munawarah 42353, Saudi ArabiaBotany and Microbiology Department, Science College, King Saud University, Riyadh 12372, Saudi ArabiaVaccines and Immunotherapy Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy, Taibah University, Al-Madinah Al-Munawarah 42353, Saudi ArabiaMedical Laboratories Technology Department, College of Applied Medical Sciences, Taibah University, Al-Madinah Al-Munawarah 42353, Saudi ArabiaDepartment of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy, Taibah University, Al-Madinah Al-Munawarah 42353, Saudi ArabiaMedical Laboratories Technology Department, College of Applied Medical Sciences, Taibah University, Al-Madinah Al-Munawarah 42353, Saudi ArabiaDepartment of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy, Taibah University, Al-Madinah Al-Munawarah 42353, Saudi ArabiaDepartment of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy, Taibah University, Al-Madinah Al-Munawarah 42353, Saudi ArabiaDepartment of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy, Taibah University, Al-Madinah Al-Munawarah 42353, Saudi ArabiaDepartment of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy, Taibah University, Al-Madinah Al-Munawarah 42353, Saudi ArabiaDepartment of Clinical Oncology, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptDepartment of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy, Taibah University, Al-Madinah Al-Munawarah 42353, Saudi ArabiaColorectal cancer (CRC) is a significant global health concern. Microbial dysbiosis and associated metabolites have been associated with CRC occurrence and progression. This study aims to analyze the gut microbiota composition and the enriched metabolic pathways in patients with late-stage CRC. In this study, a cohort of 25 CRC patients diagnosed at late stage III and IV and 25 healthy participants were enrolled. The fecal bacterial composition was investigated using V3-V4 ribosomal RNA gene sequencing, followed by clustering and linear discriminant analysis (LDA) effect size (LEfSe) analyses. A cluster of ortholog genes’ (COG) functional annotations and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were employed to identify enrichment pathways between the two groups. The findings showed that the fecal microbiota between the two groups varied significantly in alpha and beta diversities. CRC patients’ fecal samples had significantly enriched populations of <i>Streptococcus salivarius</i>, <i>S. parasanguins</i>, <i>S. anginosus</i>, <i>Lactobacillus mucosae</i>, <i>L. gasseri</i>, <i>Peptostreptococcus</i>, <i>Eubacterium</i>, <i>Aerococcus</i>, Family XIII_AD3001 Group, <i>Erysipelatoclostridium</i>, <i>Escherichia-Shigella</i>, <i>Klebsiella</i>, <i>Enterobacter</i>, <i>Alistipes</i>, <i>Ralstonia</i>, and <i>Pseudomonas</i> (Q < 0.05). The enriched pathways identified in the CRC group were amino acid transport, signaling and metabolism, membrane biogenesis, DNA replication and mismatch repair system, and protease activity (Q < 0.05). These results suggested that the imbalance between intestinal bacteria and the elevated level of the predicated functions and pathways may contribute to the development of advanced CRC tumors. Further research is warranted to elucidate the exact role of the gut microbiome in CRC and its potential implications for use in diagnostic, prevention, and treatment strategies.https://www.mdpi.com/2072-6694/15/20/5019colorectal cancer (CRC)microbiota16s rRNAgenomedysbiosisbacteria |
| spellingShingle | Areej A. Alhhazmi Yahya A. Almutawif Walaa A. Mumena Shaima M. Alhazmi Turki S. Abujamel Ruba M. Alhusayni Raghad Aloufi Razan R. Al-Hejaili Rahaf Alhujaily Lama M. Alrehaili Ruya A. Alsaedy Rahaf H. Khoja Wassal Ahmed Mohamed F. Abdelmohsen Waleed Mohammed-Saeid Identification of Gut Microbiota Profile Associated with Colorectal Cancer in Saudi Population Cancers colorectal cancer (CRC) microbiota 16s rRNA genome dysbiosis bacteria |
| title | Identification of Gut Microbiota Profile Associated with Colorectal Cancer in Saudi Population |
| title_full | Identification of Gut Microbiota Profile Associated with Colorectal Cancer in Saudi Population |
| title_fullStr | Identification of Gut Microbiota Profile Associated with Colorectal Cancer in Saudi Population |
| title_full_unstemmed | Identification of Gut Microbiota Profile Associated with Colorectal Cancer in Saudi Population |
| title_short | Identification of Gut Microbiota Profile Associated with Colorectal Cancer in Saudi Population |
| title_sort | identification of gut microbiota profile associated with colorectal cancer in saudi population |
| topic | colorectal cancer (CRC) microbiota 16s rRNA genome dysbiosis bacteria |
| url | https://www.mdpi.com/2072-6694/15/20/5019 |
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