Carbon dioxide treatment modulates phosphatidic acid signaling and stress response to improve chilling tolerance and postharvest quality in paprika
IntroductionPaprika (Capsicum annuum L.) is prone to chilling injury (CI) during low-temperature storage. Although recent findings suggest that CO2 treatment may protect against CI, the effects of short-term CO2 treatment on CI and the underlying molecular mechanisms in paprika remain unknown. There...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1287997/full |
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| author | Me-Hea Park Kang-Mo Ku Kyung-Ran Do Hyang Lan Eum Jae Han Cho Pue Hee Park Siva Kumar Malka |
| author_facet | Me-Hea Park Kang-Mo Ku Kyung-Ran Do Hyang Lan Eum Jae Han Cho Pue Hee Park Siva Kumar Malka |
| author_sort | Me-Hea Park |
| collection | DOAJ |
| description | IntroductionPaprika (Capsicum annuum L.) is prone to chilling injury (CI) during low-temperature storage. Although recent findings suggest that CO2 treatment may protect against CI, the effects of short-term CO2 treatment on CI and the underlying molecular mechanisms in paprika remain unknown. Therefore, this study aimed to examine the effect of short-term CO2 treatment on CI and postharvest quality in paprika during storage at cold storage and retail condition at physio-biochemical-molecular level.MethodsPaprika was treated with 20 and 30% CO2 for 3 h and stored at 4°C for 14 days, followed by additional storage for 2 days at 20°C (retail condition). Fruit quality parameters, including weight loss, firmness, color, and pitting were assessed, and the molecular mechanism of the treatment was elucidated using transcriptomic and metabolomic analyses.ResultsShort-term treatment with 20 and 30% CO2 effectively maintained paprika quality during cold storage and retailer conditions, with reduced surface pitting, a common symptom of CI. Additionally, transcriptomic and metabolomic analyses revealed that 20% CO2 treatment induced genes associated with biosynthesis of phosphatidic acid (PA), diacylglycerol, triacylglycerol, and stress response, metabolites associated with phasphatidyl inositol signaling, inositol phosphate metabolism, and starch and sucrose metabolism.ConclusionCO2 treatment activates PA biosynthesis through PLD and PLC-DGK pathways, and induces inositol phosphate, starch, and sucrose metabolism, thereby regulating chilling stress response via the ICE-CBF pathway. These findings suggest that short-term CO2 treatment enhances resistance to cold-induced injury and preserves postharvest quality in non-climacteric fruits, such as paprika, through activation of PA signaling, which improves membrane stability during cold storage and distribution. |
| first_indexed | 2024-03-09T14:43:39Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-09T14:43:39Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-9431e350b0014162a06347715facd24c2023-11-27T12:02:16ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-11-011410.3389/fpls.2023.12879971287997Carbon dioxide treatment modulates phosphatidic acid signaling and stress response to improve chilling tolerance and postharvest quality in paprikaMe-Hea Park0Kang-Mo Ku1Kyung-Ran Do2Hyang Lan Eum3Jae Han Cho4Pue Hee Park5Siva Kumar Malka6Postharvest Research Division, National Institute of Horticultural and Herbal Science, Wanju, Republic of KoreaDepartment of Plant Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of KoreaPlanning and Coordination Division, National Institute of Horticultural & Herbal Science, Wanju, Republic of KoreaPostharvest Research Division, National Institute of Horticultural and Herbal Science, Wanju, Republic of KoreaPostharvest Research Division, National Institute of Horticultural and Herbal Science, Wanju, Republic of KoreaPostharvest Research Division, National Institute of Horticultural and Herbal Science, Wanju, Republic of KoreaPostharvest Research Division, National Institute of Horticultural and Herbal Science, Wanju, Republic of KoreaIntroductionPaprika (Capsicum annuum L.) is prone to chilling injury (CI) during low-temperature storage. Although recent findings suggest that CO2 treatment may protect against CI, the effects of short-term CO2 treatment on CI and the underlying molecular mechanisms in paprika remain unknown. Therefore, this study aimed to examine the effect of short-term CO2 treatment on CI and postharvest quality in paprika during storage at cold storage and retail condition at physio-biochemical-molecular level.MethodsPaprika was treated with 20 and 30% CO2 for 3 h and stored at 4°C for 14 days, followed by additional storage for 2 days at 20°C (retail condition). Fruit quality parameters, including weight loss, firmness, color, and pitting were assessed, and the molecular mechanism of the treatment was elucidated using transcriptomic and metabolomic analyses.ResultsShort-term treatment with 20 and 30% CO2 effectively maintained paprika quality during cold storage and retailer conditions, with reduced surface pitting, a common symptom of CI. Additionally, transcriptomic and metabolomic analyses revealed that 20% CO2 treatment induced genes associated with biosynthesis of phosphatidic acid (PA), diacylglycerol, triacylglycerol, and stress response, metabolites associated with phasphatidyl inositol signaling, inositol phosphate metabolism, and starch and sucrose metabolism.ConclusionCO2 treatment activates PA biosynthesis through PLD and PLC-DGK pathways, and induces inositol phosphate, starch, and sucrose metabolism, thereby regulating chilling stress response via the ICE-CBF pathway. These findings suggest that short-term CO2 treatment enhances resistance to cold-induced injury and preserves postharvest quality in non-climacteric fruits, such as paprika, through activation of PA signaling, which improves membrane stability during cold storage and distribution.https://www.frontiersin.org/articles/10.3389/fpls.2023.1287997/fullCapsicum annum L.chilling injurymembrane integritypostharvest qualitylipid metabolismphosphatidic acid |
| spellingShingle | Me-Hea Park Kang-Mo Ku Kyung-Ran Do Hyang Lan Eum Jae Han Cho Pue Hee Park Siva Kumar Malka Carbon dioxide treatment modulates phosphatidic acid signaling and stress response to improve chilling tolerance and postharvest quality in paprika Frontiers in Plant Science Capsicum annum L. chilling injury membrane integrity postharvest quality lipid metabolism phosphatidic acid |
| title | Carbon dioxide treatment modulates phosphatidic acid signaling and stress response to improve chilling tolerance and postharvest quality in paprika |
| title_full | Carbon dioxide treatment modulates phosphatidic acid signaling and stress response to improve chilling tolerance and postharvest quality in paprika |
| title_fullStr | Carbon dioxide treatment modulates phosphatidic acid signaling and stress response to improve chilling tolerance and postharvest quality in paprika |
| title_full_unstemmed | Carbon dioxide treatment modulates phosphatidic acid signaling and stress response to improve chilling tolerance and postharvest quality in paprika |
| title_short | Carbon dioxide treatment modulates phosphatidic acid signaling and stress response to improve chilling tolerance and postharvest quality in paprika |
| title_sort | carbon dioxide treatment modulates phosphatidic acid signaling and stress response to improve chilling tolerance and postharvest quality in paprika |
| topic | Capsicum annum L. chilling injury membrane integrity postharvest quality lipid metabolism phosphatidic acid |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1287997/full |
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