Direct Extraction of Fumaric Acid from <i>Rhizopus oryzae</i> Suspensions—Interfacial Mass Transfer
Experimental studies on the reactive extraction of fumaric acid with Amberlite LA-2 from <i>Rhizopus oryzae</i> suspensions using three solvents with different dielectric constants varying from 9.08 to 1.90 (dichloromethane, n-butyl acetate, and n-heptane, respectively) underlined the pa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/11/1563 |
| _version_ | 1797511085780107264 |
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| author | Dan Cascaval Anca-Irina Galaction Alexandra Tucaliuc Lenuta Kloetzer |
| author_facet | Dan Cascaval Anca-Irina Galaction Alexandra Tucaliuc Lenuta Kloetzer |
| author_sort | Dan Cascaval |
| collection | DOAJ |
| description | Experimental studies on the reactive extraction of fumaric acid with Amberlite LA-2 from <i>Rhizopus oryzae</i> suspensions using three solvents with different dielectric constants varying from 9.08 to 1.90 (dichloromethane, n-butyl acetate, and n-heptane, respectively) underlined the particular behavior of the extraction system in the presence of fungal biomass. The interfacial mass flow of the reaction product was found to be significantly affected by the biomass, due mainly to its adsorption onto the phase separation interface, this leading to the appearance of a physical barrier against the solute’s transfer. However, the magnitude of the adsorption phenomenon was found to depend on <i>Rhizopus oryzae</i>’s affinity for the solvent phase, which increased significantly from dichloromethane to n-heptane. The negative influence of the biomass on the interfacial mass transfer can be partially counteracted by adding 1-octanol into the organic phase, improving the solvent’s ability to solve the fumaric acid–Amberlite LA-2 complex and simplifying the reactive extraction mechanism, effects that were found to be more important for low-polar solvents. Consequently, for the same mixing intensity, the maximum amplification factor was reached for n-heptane, its value being almost 5–6 times higher than that obtained for dichloromethane and over 2 times higher than that obtained with n-butyl acetate. |
| first_indexed | 2024-03-10T05:41:26Z |
| format | Article |
| id | doaj.art-94320d2cf36c4591b3dadccaa9df4e8f |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T05:41:26Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-94320d2cf36c4591b3dadccaa9df4e8f2023-11-22T22:33:01ZengMDPI AGBiomolecules2218-273X2021-10-011111156310.3390/biom11111563Direct Extraction of Fumaric Acid from <i>Rhizopus oryzae</i> Suspensions—Interfacial Mass TransferDan Cascaval0Anca-Irina Galaction1Alexandra Tucaliuc2Lenuta Kloetzer3Cristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University, D. Mangeron 73, 700050 Iasi, RomaniaFaculty of Medical Bioengineering, Grigore T. Popa University of Medicine and Pharmacy, M. Kogalniceanu 9-13, 700454 Iasi, RomaniaCristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University, D. Mangeron 73, 700050 Iasi, RomaniaCristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University, D. Mangeron 73, 700050 Iasi, RomaniaExperimental studies on the reactive extraction of fumaric acid with Amberlite LA-2 from <i>Rhizopus oryzae</i> suspensions using three solvents with different dielectric constants varying from 9.08 to 1.90 (dichloromethane, n-butyl acetate, and n-heptane, respectively) underlined the particular behavior of the extraction system in the presence of fungal biomass. The interfacial mass flow of the reaction product was found to be significantly affected by the biomass, due mainly to its adsorption onto the phase separation interface, this leading to the appearance of a physical barrier against the solute’s transfer. However, the magnitude of the adsorption phenomenon was found to depend on <i>Rhizopus oryzae</i>’s affinity for the solvent phase, which increased significantly from dichloromethane to n-heptane. The negative influence of the biomass on the interfacial mass transfer can be partially counteracted by adding 1-octanol into the organic phase, improving the solvent’s ability to solve the fumaric acid–Amberlite LA-2 complex and simplifying the reactive extraction mechanism, effects that were found to be more important for low-polar solvents. Consequently, for the same mixing intensity, the maximum amplification factor was reached for n-heptane, its value being almost 5–6 times higher than that obtained for dichloromethane and over 2 times higher than that obtained with n-butyl acetate.https://www.mdpi.com/2218-273X/11/11/1563fumaric acidAmberlite LA-21-octanolreactive extractionmass flow<i>Rhizopus oryzae</i> |
| spellingShingle | Dan Cascaval Anca-Irina Galaction Alexandra Tucaliuc Lenuta Kloetzer Direct Extraction of Fumaric Acid from <i>Rhizopus oryzae</i> Suspensions—Interfacial Mass Transfer Biomolecules fumaric acid Amberlite LA-2 1-octanol reactive extraction mass flow <i>Rhizopus oryzae</i> |
| title | Direct Extraction of Fumaric Acid from <i>Rhizopus oryzae</i> Suspensions—Interfacial Mass Transfer |
| title_full | Direct Extraction of Fumaric Acid from <i>Rhizopus oryzae</i> Suspensions—Interfacial Mass Transfer |
| title_fullStr | Direct Extraction of Fumaric Acid from <i>Rhizopus oryzae</i> Suspensions—Interfacial Mass Transfer |
| title_full_unstemmed | Direct Extraction of Fumaric Acid from <i>Rhizopus oryzae</i> Suspensions—Interfacial Mass Transfer |
| title_short | Direct Extraction of Fumaric Acid from <i>Rhizopus oryzae</i> Suspensions—Interfacial Mass Transfer |
| title_sort | direct extraction of fumaric acid from i rhizopus oryzae i suspensions interfacial mass transfer |
| topic | fumaric acid Amberlite LA-2 1-octanol reactive extraction mass flow <i>Rhizopus oryzae</i> |
| url | https://www.mdpi.com/2218-273X/11/11/1563 |
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