Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats
Fasting is known to cause physiological changes in the endocrine pancreas, including decreased insulin secretion and increased reactive oxygen species (ROS) production. However, there is no consensus about the long-term effects of intermittent fasting (IF), which can involve up to 24 hours of fastin...
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MDPI AG
2020-04-01
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author | Ana Cláudia Munhoz Eloisa Aparecida Vilas-Boas Ana Carolina Panveloski-Costa Jaqueline Santos Moreira Leite Camila Ferraz Lucena Patrícia Riva Henriette Emilio Angelo R. Carpinelli |
author_facet | Ana Cláudia Munhoz Eloisa Aparecida Vilas-Boas Ana Carolina Panveloski-Costa Jaqueline Santos Moreira Leite Camila Ferraz Lucena Patrícia Riva Henriette Emilio Angelo R. Carpinelli |
author_sort | Ana Cláudia Munhoz |
collection | DOAJ |
description | Fasting is known to cause physiological changes in the endocrine pancreas, including decreased insulin secretion and increased reactive oxygen species (ROS) production. However, there is no consensus about the long-term effects of intermittent fasting (IF), which can involve up to 24 hours of fasting interspersed with normal feeding days. In the present study, we analyzed the effects of alternate-day IF for 12 weeks in a developing and healthy organism. Female 30-day-old Wistar rats were randomly divided into two groups: control, with free access to standard rodent chow; and IF, subjected to 24-hour fasts intercalated with 24-hours of free access to the same chow. Alternate-day IF decreased weight gain and food intake. Surprisingly, IF also elevated plasma insulin concentrations, both at baseline and after glucose administration collected during oGTT. After 12 weeks of dietary intervention, pancreatic islets displayed increased ROS production and apoptosis. Despite their lower body weight, IF animals had increased fat reserves and decreased muscle mass. Taken together, these findings suggest that alternate-day IF promote β -cell dysfunction, especially in developing animals. More long-term research is necessary to define the best IF protocol to reduce side effects. |
first_indexed | 2024-03-10T20:34:47Z |
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issn | 2072-6643 |
language | English |
last_indexed | 2024-03-10T20:34:47Z |
publishDate | 2020-04-01 |
publisher | MDPI AG |
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series | Nutrients |
spelling | doaj.art-943448ef2548437a951bb7c00df9714a2023-11-19T21:07:28ZengMDPI AGNutrients2072-66432020-04-01124102910.3390/nu12041029Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar RatsAna Cláudia Munhoz0Eloisa Aparecida Vilas-Boas1Ana Carolina Panveloski-Costa2Jaqueline Santos Moreira Leite3Camila Ferraz Lucena4Patrícia Riva5Henriette Emilio6Angelo R. Carpinelli7Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, 1524 Professor Lineu Prestes avenue, Butanta, São Paulo 05508-900, BrazilDepartment of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, 1524 Professor Lineu Prestes avenue, Butanta, São Paulo 05508-900, BrazilDepartment of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, 1524 Professor Lineu Prestes avenue, Butanta, São Paulo 05508-900, BrazilDepartment of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, 1524 Professor Lineu Prestes avenue, Butanta, São Paulo 05508-900, BrazilDepartment of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, 1524 Professor Lineu Prestes avenue, Butanta, São Paulo 05508-900, BrazilDepartment of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, 1524 Professor Lineu Prestes avenue, Butanta, São Paulo 05508-900, BrazilDepartment of General Biology, Ponta Grossa State University, 4748 General Carlos Cavalcanti avenue, Uvaranas, Parana, PR 84030-900, BrazilDepartment of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, 1524 Professor Lineu Prestes avenue, Butanta, São Paulo 05508-900, BrazilFasting is known to cause physiological changes in the endocrine pancreas, including decreased insulin secretion and increased reactive oxygen species (ROS) production. However, there is no consensus about the long-term effects of intermittent fasting (IF), which can involve up to 24 hours of fasting interspersed with normal feeding days. In the present study, we analyzed the effects of alternate-day IF for 12 weeks in a developing and healthy organism. Female 30-day-old Wistar rats were randomly divided into two groups: control, with free access to standard rodent chow; and IF, subjected to 24-hour fasts intercalated with 24-hours of free access to the same chow. Alternate-day IF decreased weight gain and food intake. Surprisingly, IF also elevated plasma insulin concentrations, both at baseline and after glucose administration collected during oGTT. After 12 weeks of dietary intervention, pancreatic islets displayed increased ROS production and apoptosis. Despite their lower body weight, IF animals had increased fat reserves and decreased muscle mass. Taken together, these findings suggest that alternate-day IF promote β -cell dysfunction, especially in developing animals. More long-term research is necessary to define the best IF protocol to reduce side effects.https://www.mdpi.com/2072-6643/12/4/1029intermittent fastingfat massinsulin secretionpancreatic islet |
spellingShingle | Ana Cláudia Munhoz Eloisa Aparecida Vilas-Boas Ana Carolina Panveloski-Costa Jaqueline Santos Moreira Leite Camila Ferraz Lucena Patrícia Riva Henriette Emilio Angelo R. Carpinelli Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats Nutrients intermittent fasting fat mass insulin secretion pancreatic islet |
title | Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats |
title_full | Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats |
title_fullStr | Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats |
title_full_unstemmed | Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats |
title_short | Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats |
title_sort | intermittent fasting for twelve weeks leads to increases in fat mass and hyperinsulinemia in young female wistar rats |
topic | intermittent fasting fat mass insulin secretion pancreatic islet |
url | https://www.mdpi.com/2072-6643/12/4/1029 |
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