Alterations in Oxidative Stress Markers and Na,K-ATPase Enzyme Properties in Kidney after Fructose Intake and Quercetin Intervention in Rats
The study aimed to characterize the consequences of a 15-week intake of 10% fructose on the kidney, with the focus on oxidative stress markers and properties of the Na,K-ATPase enzyme. Various antioxidants naturally occurring in common food were demonstrated to be protective against fructose-induced...
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MDPI AG
2023-04-01
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| Online Access: | https://www.mdpi.com/2075-1729/13/4/931 |
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| author | Norbert Vrbjar Jana Vlkovicova Denisa Snurikova Barbora Kalocayova Stefan Zorad Tijana Culafic Snezana Tepavcevic Lubomira Tothova Dominika Radosinska Marta Kollarova Jana Radosinska |
| author_facet | Norbert Vrbjar Jana Vlkovicova Denisa Snurikova Barbora Kalocayova Stefan Zorad Tijana Culafic Snezana Tepavcevic Lubomira Tothova Dominika Radosinska Marta Kollarova Jana Radosinska |
| author_sort | Norbert Vrbjar |
| collection | DOAJ |
| description | The study aimed to characterize the consequences of a 15-week intake of 10% fructose on the kidney, with the focus on oxidative stress markers and properties of the Na,K-ATPase enzyme. Various antioxidants naturally occurring in common food were demonstrated to be protective against fructose-induced deterioration of kidneys. Therefore, we also aimed to observe the effect of 6-week quercetin administration (20 mg/kg/day) that was initiated following the 9-week period of higher fructose intake, by determining the concentration of sodium, potassium, creatinine, urea, and glucose in blood plasma and oxidative status directly in the renal tissue. Kinetic studies of renal Na,K-ATPase were utilized for a deeper insight into the molecular principles of expected changes in this enzyme activity under conditions of presumed fructose-induced renal injury. Fructose intake led to increase in body weight gain, plasma glucose and sodium levels, and deterioration of kidney properties, although some compensatory mechanisms were observable. Quercetin administration improved glycemic control in rats exposed to fructose overload. However, an increase in plasma creatinine, a decrease in GSH/GSSG ratio in renal tissue homogenate, and a controversial effect on renal Na,K-ATPase enzyme suggest that quercetin treatment may not be beneficial in the condition of pre-existing renal pathology. |
| first_indexed | 2024-03-11T04:50:11Z |
| format | Article |
| id | doaj.art-94383f86af3d47dfa70f21e78cb3773a |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T04:50:11Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-94383f86af3d47dfa70f21e78cb3773a2023-11-17T20:05:38ZengMDPI AGLife2075-17292023-04-0113493110.3390/life13040931Alterations in Oxidative Stress Markers and Na,K-ATPase Enzyme Properties in Kidney after Fructose Intake and Quercetin Intervention in RatsNorbert Vrbjar0Jana Vlkovicova1Denisa Snurikova2Barbora Kalocayova3Stefan Zorad4Tijana Culafic5Snezana Tepavcevic6Lubomira Tothova7Dominika Radosinska8Marta Kollarova9Jana Radosinska10Centre of Experimental Medicine, Slovak Academy of Sciences, Institute for Heart Research, Dúbravská Cesta 9, 841 04 Bratislava, SlovakiaCentre of Experimental Medicine, Slovak Academy of Sciences, Institute for Heart Research, Dúbravská Cesta 9, 841 04 Bratislava, SlovakiaCentre of Experimental Medicine, Slovak Academy of Sciences, Institute for Heart Research, Dúbravská Cesta 9, 841 04 Bratislava, SlovakiaCentre of Experimental Medicine, Slovak Academy of Sciences, Institute for Heart Research, Dúbravská Cesta 9, 841 04 Bratislava, SlovakiaInstitute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, 845 05 Bratislava, SlovakiaLaboratory for Molecular Biology and Endocrinology, “VINČA” Institute of Nuclear Sciences—National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Molecular Biology and Endocrinology, “VINČA” Institute of Nuclear Sciences—National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaInstitute of Molecular Biomedicine, Faculty of Medicine, Comenius University in Bratislava, 811 08 Bratislava, SlovakiaInstitute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 4, 811 08 Bratislava, SlovakiaInstitute of Physiology, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 2, 811 08 Bratislava, SlovakiaCentre of Experimental Medicine, Slovak Academy of Sciences, Institute for Heart Research, Dúbravská Cesta 9, 841 04 Bratislava, SlovakiaThe study aimed to characterize the consequences of a 15-week intake of 10% fructose on the kidney, with the focus on oxidative stress markers and properties of the Na,K-ATPase enzyme. Various antioxidants naturally occurring in common food were demonstrated to be protective against fructose-induced deterioration of kidneys. Therefore, we also aimed to observe the effect of 6-week quercetin administration (20 mg/kg/day) that was initiated following the 9-week period of higher fructose intake, by determining the concentration of sodium, potassium, creatinine, urea, and glucose in blood plasma and oxidative status directly in the renal tissue. Kinetic studies of renal Na,K-ATPase were utilized for a deeper insight into the molecular principles of expected changes in this enzyme activity under conditions of presumed fructose-induced renal injury. Fructose intake led to increase in body weight gain, plasma glucose and sodium levels, and deterioration of kidney properties, although some compensatory mechanisms were observable. Quercetin administration improved glycemic control in rats exposed to fructose overload. However, an increase in plasma creatinine, a decrease in GSH/GSSG ratio in renal tissue homogenate, and a controversial effect on renal Na,K-ATPase enzyme suggest that quercetin treatment may not be beneficial in the condition of pre-existing renal pathology.https://www.mdpi.com/2075-1729/13/4/931fructosequercetinkidneyoxidative stressNa,K-ATPaseglycemia |
| spellingShingle | Norbert Vrbjar Jana Vlkovicova Denisa Snurikova Barbora Kalocayova Stefan Zorad Tijana Culafic Snezana Tepavcevic Lubomira Tothova Dominika Radosinska Marta Kollarova Jana Radosinska Alterations in Oxidative Stress Markers and Na,K-ATPase Enzyme Properties in Kidney after Fructose Intake and Quercetin Intervention in Rats Life fructose quercetin kidney oxidative stress Na,K-ATPase glycemia |
| title | Alterations in Oxidative Stress Markers and Na,K-ATPase Enzyme Properties in Kidney after Fructose Intake and Quercetin Intervention in Rats |
| title_full | Alterations in Oxidative Stress Markers and Na,K-ATPase Enzyme Properties in Kidney after Fructose Intake and Quercetin Intervention in Rats |
| title_fullStr | Alterations in Oxidative Stress Markers and Na,K-ATPase Enzyme Properties in Kidney after Fructose Intake and Quercetin Intervention in Rats |
| title_full_unstemmed | Alterations in Oxidative Stress Markers and Na,K-ATPase Enzyme Properties in Kidney after Fructose Intake and Quercetin Intervention in Rats |
| title_short | Alterations in Oxidative Stress Markers and Na,K-ATPase Enzyme Properties in Kidney after Fructose Intake and Quercetin Intervention in Rats |
| title_sort | alterations in oxidative stress markers and na k atpase enzyme properties in kidney after fructose intake and quercetin intervention in rats |
| topic | fructose quercetin kidney oxidative stress Na,K-ATPase glycemia |
| url | https://www.mdpi.com/2075-1729/13/4/931 |
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