Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and Tissue
Psoriasis is a frequent and incurable skin disease whose pathogenesis is still not fully understood. It is characterized by immune disturbances leading to hyperproliferation and improper differentiation of keratinocytes. Gasdermin E (GSDME) is a protein from the gasdermin family involved in the proc...
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MDPI AG
2023-08-01
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| author | Julia Nowowiejska Anna Baran Anna Pryczynicz Justyna Magdalena Hermanowicz Beata Sieklucka Dariusz Pawlak Iwona Flisiak |
| author_facet | Julia Nowowiejska Anna Baran Anna Pryczynicz Justyna Magdalena Hermanowicz Beata Sieklucka Dariusz Pawlak Iwona Flisiak |
| author_sort | Julia Nowowiejska |
| collection | DOAJ |
| description | Psoriasis is a frequent and incurable skin disease whose pathogenesis is still not fully understood. It is characterized by immune disturbances leading to hyperproliferation and improper differentiation of keratinocytes. Gasdermin E (GSDME) is a protein from the gasdermin family involved in the processes of inflammation and cell death based on apoptosis, necroptosis and pyroptosis. It has never been studied in psoriatics’ sera or urine before. Our study enrolled 60 patients with psoriasis and 30 volunteers without dermatoses as controls. Serum and urinary GSDME concentrations were examined by ELISA and tissue expression of GSDME by immunohistochemistry. Serum GSDME concentration was significantly higher in patients than controls (<i>p</i> < 0.05). There were no differences in urinary GSDME concentrations between patients and controls. GSDME expression was significantly higher in the psoriatic plaque than non-lesional patients’ skin and compared to controls (both <i>p</i> < 0.001). There was no correlation between serum GSDME or its lesional expression and psoriasis severity, age or disease duration. GSDME serum concentration was significantly negatively correlated with BMI, triglycerides and glucose concentrations. The obtained results suggest the engagement of GSDME in psoriasis pathogenesis. It could potentially become a new non-invasive psoriasis marker. Considering its pro-apoptotic influence, GSDME could be compensatively elevated to direct cells towards apoptosis, whereas under other circumstances, it may lead to pyroptosis and sustain inflammation. GSDME may exert a protective influence on the metabolic complications in psoriasis which requires further studies. |
| first_indexed | 2024-03-10T23:25:55Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T23:25:55Z |
| publishDate | 2023-08-01 |
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| series | Cells |
| spelling | doaj.art-9438e92f4cc2412ead348ec8b4aa2b1c2023-11-19T07:57:56ZengMDPI AGCells2073-44092023-08-011217214910.3390/cells12172149Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and TissueJulia Nowowiejska0Anna Baran1Anna Pryczynicz2Justyna Magdalena Hermanowicz3Beata Sieklucka4Dariusz Pawlak5Iwona Flisiak6Department of Dermatology and Venereology, Medical University of Bialystok, 14 Zurawia St., 15-540 Bialystok, PolandDepartment of Dermatology and Venereology, Medical University of Bialystok, 14 Zurawia St., 15-540 Bialystok, PolandDepartment of General Pathomorphology, Medical University of Bialystok, 13 Waszyngtona St., 15-269 Bialystok, PolandDepartment of Pharmacodynamics, Medical University of Bialystok, 2C Mickiewicza St., 15-089 Bialystok, PolandDepartment of Pharmacodynamics, Medical University of Bialystok, 2C Mickiewicza St., 15-089 Bialystok, PolandDepartment of Pharmacodynamics, Medical University of Bialystok, 2C Mickiewicza St., 15-089 Bialystok, PolandDepartment of Dermatology and Venereology, Medical University of Bialystok, 14 Zurawia St., 15-540 Bialystok, PolandPsoriasis is a frequent and incurable skin disease whose pathogenesis is still not fully understood. It is characterized by immune disturbances leading to hyperproliferation and improper differentiation of keratinocytes. Gasdermin E (GSDME) is a protein from the gasdermin family involved in the processes of inflammation and cell death based on apoptosis, necroptosis and pyroptosis. It has never been studied in psoriatics’ sera or urine before. Our study enrolled 60 patients with psoriasis and 30 volunteers without dermatoses as controls. Serum and urinary GSDME concentrations were examined by ELISA and tissue expression of GSDME by immunohistochemistry. Serum GSDME concentration was significantly higher in patients than controls (<i>p</i> < 0.05). There were no differences in urinary GSDME concentrations between patients and controls. GSDME expression was significantly higher in the psoriatic plaque than non-lesional patients’ skin and compared to controls (both <i>p</i> < 0.001). There was no correlation between serum GSDME or its lesional expression and psoriasis severity, age or disease duration. GSDME serum concentration was significantly negatively correlated with BMI, triglycerides and glucose concentrations. The obtained results suggest the engagement of GSDME in psoriasis pathogenesis. It could potentially become a new non-invasive psoriasis marker. Considering its pro-apoptotic influence, GSDME could be compensatively elevated to direct cells towards apoptosis, whereas under other circumstances, it may lead to pyroptosis and sustain inflammation. GSDME may exert a protective influence on the metabolic complications in psoriasis which requires further studies.https://www.mdpi.com/2073-4409/12/17/2149GSDMEgasdermingasdermin Epyroptosisapoptosisinflammation |
| spellingShingle | Julia Nowowiejska Anna Baran Anna Pryczynicz Justyna Magdalena Hermanowicz Beata Sieklucka Dariusz Pawlak Iwona Flisiak Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and Tissue Cells GSDME gasdermin gasdermin E pyroptosis apoptosis inflammation |
| title | Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and Tissue |
| title_full | Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and Tissue |
| title_fullStr | Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and Tissue |
| title_full_unstemmed | Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and Tissue |
| title_short | Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and Tissue |
| title_sort | gasdermin e gsdme a new potential marker of psoriasis and its metabolic complications the first combined study on human serum urine and tissue |
| topic | GSDME gasdermin gasdermin E pyroptosis apoptosis inflammation |
| url | https://www.mdpi.com/2073-4409/12/17/2149 |
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