Gold Nanocluster Decorated Polypeptide/DNA Complexes for NIR Light and Redox Dual-Responsive Gene Transfection
Endo/lysosomal escape and subsequent nuclear translocation are recognized as the two major challenges for efficient gene transfection. Herein, nuclear localization signal (NLS) peptide sequences and oligomeric lysine sequences were crosslinked via disulfide bonds to obtain glutathione (GSH) reducibl...
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| Format: | Article |
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MDPI AG
2016-08-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/21/8/1103 |
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| author | Qi Lei Jing-Jing Hu Lei Rong Han Cheng Yun-Xia Sun Xian-Zheng Zhang |
| author_facet | Qi Lei Jing-Jing Hu Lei Rong Han Cheng Yun-Xia Sun Xian-Zheng Zhang |
| author_sort | Qi Lei |
| collection | DOAJ |
| description | Endo/lysosomal escape and subsequent nuclear translocation are recognized as the two major challenges for efficient gene transfection. Herein, nuclear localization signal (NLS) peptide sequences and oligomeric lysine sequences were crosslinked via disulfide bonds to obtain glutathione (GSH) reducible polypeptide (pNLS). The pNLS could condense DNA into compact positive-charged complexes with redox sensitivity, and then gold nanoclusters (AuNC) were further decorated to the surface via electrostatic interactions obtaining versatile pNLS/DNA/AuNC complexes. The AuNC could generate reactive oxygen species (ROS) under NIR-irradiation and accelerate the endo/lysosomal escape of the complexes, and then the pNLS sequence degraded by GSH in cytoplasm would release the DNA and facilitate the subsequent nuclear translocation for enhanced gene transfection. |
| first_indexed | 2024-12-10T18:01:20Z |
| format | Article |
| id | doaj.art-943971b7742b47a5b22b416def0a746c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T18:01:20Z |
| publishDate | 2016-08-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-943971b7742b47a5b22b416def0a746c2022-12-22T01:38:45ZengMDPI AGMolecules1420-30492016-08-01218110310.3390/molecules21081103molecules21081103Gold Nanocluster Decorated Polypeptide/DNA Complexes for NIR Light and Redox Dual-Responsive Gene TransfectionQi Lei0Jing-Jing Hu1Lei Rong2Han Cheng3Yun-Xia Sun4Xian-Zheng Zhang5Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, ChinaKey Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, ChinaKey Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, ChinaKey Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, ChinaKey Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, ChinaKey Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, ChinaEndo/lysosomal escape and subsequent nuclear translocation are recognized as the two major challenges for efficient gene transfection. Herein, nuclear localization signal (NLS) peptide sequences and oligomeric lysine sequences were crosslinked via disulfide bonds to obtain glutathione (GSH) reducible polypeptide (pNLS). The pNLS could condense DNA into compact positive-charged complexes with redox sensitivity, and then gold nanoclusters (AuNC) were further decorated to the surface via electrostatic interactions obtaining versatile pNLS/DNA/AuNC complexes. The AuNC could generate reactive oxygen species (ROS) under NIR-irradiation and accelerate the endo/lysosomal escape of the complexes, and then the pNLS sequence degraded by GSH in cytoplasm would release the DNA and facilitate the subsequent nuclear translocation for enhanced gene transfection.http://www.mdpi.com/1420-3049/21/8/1103NIR-responsiveredox-responsivenuclear localization signalendo/lysosomal escapegene delivery |
| spellingShingle | Qi Lei Jing-Jing Hu Lei Rong Han Cheng Yun-Xia Sun Xian-Zheng Zhang Gold Nanocluster Decorated Polypeptide/DNA Complexes for NIR Light and Redox Dual-Responsive Gene Transfection Molecules NIR-responsive redox-responsive nuclear localization signal endo/lysosomal escape gene delivery |
| title | Gold Nanocluster Decorated Polypeptide/DNA Complexes for NIR Light and Redox Dual-Responsive Gene Transfection |
| title_full | Gold Nanocluster Decorated Polypeptide/DNA Complexes for NIR Light and Redox Dual-Responsive Gene Transfection |
| title_fullStr | Gold Nanocluster Decorated Polypeptide/DNA Complexes for NIR Light and Redox Dual-Responsive Gene Transfection |
| title_full_unstemmed | Gold Nanocluster Decorated Polypeptide/DNA Complexes for NIR Light and Redox Dual-Responsive Gene Transfection |
| title_short | Gold Nanocluster Decorated Polypeptide/DNA Complexes for NIR Light and Redox Dual-Responsive Gene Transfection |
| title_sort | gold nanocluster decorated polypeptide dna complexes for nir light and redox dual responsive gene transfection |
| topic | NIR-responsive redox-responsive nuclear localization signal endo/lysosomal escape gene delivery |
| url | http://www.mdpi.com/1420-3049/21/8/1103 |
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