Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from <i>Lactobacillus</i> Strains
<i>Lactobacillus</i> are normal inhabitants of the gastrointestinal tract and confer a variety of health effects. Lipoteichoic acid (LTA), an amphiphilic substance located in the cell membrane, is a key molecule in probiotic–host crosstalk. Through the characterization of structural char...
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MDPI AG
2022-05-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/11/1610 |
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| author | Qianqian Lu Yingqi Guo Guo Yang Lei Cui Zhen Wu Xiaoqun Zeng Daodong Pan Zhendong Cai |
| author_facet | Qianqian Lu Yingqi Guo Guo Yang Lei Cui Zhen Wu Xiaoqun Zeng Daodong Pan Zhendong Cai |
| author_sort | Qianqian Lu |
| collection | DOAJ |
| description | <i>Lactobacillus</i> are normal inhabitants of the gastrointestinal tract and confer a variety of health effects. Lipoteichoic acid (LTA), an amphiphilic substance located in the cell membrane, is a key molecule in probiotic–host crosstalk. Through the characterization of structural characteristics of LTA molecules derived from <i>Lactobacillus plantarum</i> A3, <i>Lactobacillus reuteri</i> DMSZ 8533, and <i>Lactobacillus acidophilus</i> CICC 6074, there exists some heterogeneity in LTA molecules, which perhaps contributes to the distinguishable adhesion properties of <i>Lactobacillus</i> strains based on fluorescence microscopy observations. In LPS-induced RAW 264.7 cells, LTAs derived from three <i>Lactobacillus</i> strains obviously alleviated inflammatory responses as evidenced by the altered inflammatory cytokine levels of TNF-α, IL-6, and IL-10. Western blotting demonstrated that <i>L. reuteri</i> LTA blocked LPS-triggered expression of the MAPK and NF-κB pathways. The findings further validated that LTA is an important effector molecule and deserves further consideration as an alternative therapeutic for ulcerative colitis treatment. |
| first_indexed | 2024-03-10T01:19:10Z |
| format | Article |
| id | doaj.art-9439d581e5e7414ab409973ffa29d6c8 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T01:19:10Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-9439d581e5e7414ab409973ffa29d6c82023-11-23T14:03:03ZengMDPI AGFoods2304-81582022-05-011111161010.3390/foods11111610Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from <i>Lactobacillus</i> StrainsQianqian Lu0Yingqi Guo1Guo Yang2Lei Cui3Zhen Wu4Xiaoqun Zeng5Daodong Pan6Zhendong Cai7Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, ChinaKey Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, ChinaKey Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, ChinaKey Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, ChinaKey Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, ChinaKey Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, ChinaKey Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, ChinaKey Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China<i>Lactobacillus</i> are normal inhabitants of the gastrointestinal tract and confer a variety of health effects. Lipoteichoic acid (LTA), an amphiphilic substance located in the cell membrane, is a key molecule in probiotic–host crosstalk. Through the characterization of structural characteristics of LTA molecules derived from <i>Lactobacillus plantarum</i> A3, <i>Lactobacillus reuteri</i> DMSZ 8533, and <i>Lactobacillus acidophilus</i> CICC 6074, there exists some heterogeneity in LTA molecules, which perhaps contributes to the distinguishable adhesion properties of <i>Lactobacillus</i> strains based on fluorescence microscopy observations. In LPS-induced RAW 264.7 cells, LTAs derived from three <i>Lactobacillus</i> strains obviously alleviated inflammatory responses as evidenced by the altered inflammatory cytokine levels of TNF-α, IL-6, and IL-10. Western blotting demonstrated that <i>L. reuteri</i> LTA blocked LPS-triggered expression of the MAPK and NF-κB pathways. The findings further validated that LTA is an important effector molecule and deserves further consideration as an alternative therapeutic for ulcerative colitis treatment.https://www.mdpi.com/2304-8158/11/11/1610<i>Lactobacillus</i>lipoteichoic acidadhesionanti-inflammationMAPK and NF-κB signals |
| spellingShingle | Qianqian Lu Yingqi Guo Guo Yang Lei Cui Zhen Wu Xiaoqun Zeng Daodong Pan Zhendong Cai Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from <i>Lactobacillus</i> Strains Foods <i>Lactobacillus</i> lipoteichoic acid adhesion anti-inflammation MAPK and NF-κB signals |
| title | Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from <i>Lactobacillus</i> Strains |
| title_full | Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from <i>Lactobacillus</i> Strains |
| title_fullStr | Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from <i>Lactobacillus</i> Strains |
| title_full_unstemmed | Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from <i>Lactobacillus</i> Strains |
| title_short | Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from <i>Lactobacillus</i> Strains |
| title_sort | structure and anti inflammation potential of lipoteichoic acids isolated from i lactobacillus i strains |
| topic | <i>Lactobacillus</i> lipoteichoic acid adhesion anti-inflammation MAPK and NF-κB signals |
| url | https://www.mdpi.com/2304-8158/11/11/1610 |
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