Myonuclear transcriptional dynamics in response to exercise following satellite cell depletion
Summary: Skeletal muscle is composed of post-mitotic myofibers that form a syncytium containing hundreds of myonuclei. Using a progressive exercise training model in the mouse and single nucleus RNA-sequencing (snRNA-seq) for high-resolution characterization of myonuclear transcription, we show myon...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-08-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221008063 |
| _version_ | 1819137992332148736 |
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| author | Yuan Wen Davis A. Englund Bailey D. Peck Kevin A. Murach John J. McCarthy Charlotte A. Peterson |
| author_facet | Yuan Wen Davis A. Englund Bailey D. Peck Kevin A. Murach John J. McCarthy Charlotte A. Peterson |
| author_sort | Yuan Wen |
| collection | DOAJ |
| description | Summary: Skeletal muscle is composed of post-mitotic myofibers that form a syncytium containing hundreds of myonuclei. Using a progressive exercise training model in the mouse and single nucleus RNA-sequencing (snRNA-seq) for high-resolution characterization of myonuclear transcription, we show myonuclear functional specialization in muscle. After 4 weeks of exercise training, snRNA-seq reveals that resident muscle stem cells, or satellite cells, are activated with acute exercise but demonstrate limited lineage progression while contributing to muscle adaptation. In the absence of satellite cells, a portion of nuclei demonstrates divergent transcriptional dynamics associated with mixed-fate identities compared with satellite cell replete muscles. These data provide a compendium of information about how satellite cells influence myonuclear transcription in response to exercise. |
| first_indexed | 2024-12-22T10:59:41Z |
| format | Article |
| id | doaj.art-943dd826db33413cb54063aae105fce2 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-22T10:59:41Z |
| publishDate | 2021-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-943dd826db33413cb54063aae105fce22022-12-21T18:28:32ZengElsevieriScience2589-00422021-08-01248102838Myonuclear transcriptional dynamics in response to exercise following satellite cell depletionYuan Wen0Davis A. Englund1Bailey D. Peck2Kevin A. Murach3John J. McCarthy4Charlotte A. Peterson5Department of Physical Therapy, College of Health Sciences, University of Kentucky, 900 S. Limestone, Lexington, KY 40536-0200, USA; Center for Muscle Biology, University of Kentucky, Lexington, KY, USADepartment of Physical Therapy, College of Health Sciences, University of Kentucky, 900 S. Limestone, Lexington, KY 40536-0200, USA; Center for Muscle Biology, University of Kentucky, Lexington, KY, USADepartment of Physical Therapy, College of Health Sciences, University of Kentucky, 900 S. Limestone, Lexington, KY 40536-0200, USA; Center for Muscle Biology, University of Kentucky, Lexington, KY, USADepartment of Physical Therapy, College of Health Sciences, University of Kentucky, 900 S. Limestone, Lexington, KY 40536-0200, USA; Center for Muscle Biology, University of Kentucky, Lexington, KY, USACenter for Muscle Biology, University of Kentucky, Lexington, KY, USA; Department of Physiology, College of Medicine, University of Kentucky, Lexington, KY, USADepartment of Physical Therapy, College of Health Sciences, University of Kentucky, 900 S. Limestone, Lexington, KY 40536-0200, USA; Center for Muscle Biology, University of Kentucky, Lexington, KY, USA; Corresponding authorSummary: Skeletal muscle is composed of post-mitotic myofibers that form a syncytium containing hundreds of myonuclei. Using a progressive exercise training model in the mouse and single nucleus RNA-sequencing (snRNA-seq) for high-resolution characterization of myonuclear transcription, we show myonuclear functional specialization in muscle. After 4 weeks of exercise training, snRNA-seq reveals that resident muscle stem cells, or satellite cells, are activated with acute exercise but demonstrate limited lineage progression while contributing to muscle adaptation. In the absence of satellite cells, a portion of nuclei demonstrates divergent transcriptional dynamics associated with mixed-fate identities compared with satellite cell replete muscles. These data provide a compendium of information about how satellite cells influence myonuclear transcription in response to exercise.http://www.sciencedirect.com/science/article/pii/S2589004221008063Biological sciencesStem cells researchTranscriptomics |
| spellingShingle | Yuan Wen Davis A. Englund Bailey D. Peck Kevin A. Murach John J. McCarthy Charlotte A. Peterson Myonuclear transcriptional dynamics in response to exercise following satellite cell depletion iScience Biological sciences Stem cells research Transcriptomics |
| title | Myonuclear transcriptional dynamics in response to exercise following satellite cell depletion |
| title_full | Myonuclear transcriptional dynamics in response to exercise following satellite cell depletion |
| title_fullStr | Myonuclear transcriptional dynamics in response to exercise following satellite cell depletion |
| title_full_unstemmed | Myonuclear transcriptional dynamics in response to exercise following satellite cell depletion |
| title_short | Myonuclear transcriptional dynamics in response to exercise following satellite cell depletion |
| title_sort | myonuclear transcriptional dynamics in response to exercise following satellite cell depletion |
| topic | Biological sciences Stem cells research Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221008063 |
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