Two genotypes of infectious bronchitis virus are responsible for serological variation in KwaZulu-Natal poultry flocks prior to 2012
This study describes the isolation, serotyping and genotyping of 54 infectious bronchitis virus (IBV) cases predominantly in KwaZulu-Natal and compared to several isolates from other South African provinces between 2011 and 2012 and several historic isolates. The results indicate the division of iso...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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AOSIS
2014-09-01
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| Series: | Onderstepoort Journal of Veterinary Research |
| Subjects: | |
| Online Access: | https://ojvr.org/index.php/ojvr/article/view/769 |
| _version_ | 1818515567605186560 |
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| author | Adrian D. Knoetze Niloshni Moodley Celia Abolnik |
| author_facet | Adrian D. Knoetze Niloshni Moodley Celia Abolnik |
| author_sort | Adrian D. Knoetze |
| collection | DOAJ |
| description | This study describes the isolation, serotyping and genotyping of 54 infectious bronchitis virus (IBV) cases predominantly in KwaZulu-Natal and compared to several isolates from other South African provinces between 2011 and 2012 and several historic isolates. The results indicate the division of isolates into two different genotypes of IBV within the province, Massachusetts (Mass)-like and QX-like. The IBV Mass-like genotype was the most prevalent and was detected in 79% of the full spike protein S1 gene sequences. Variation up to 22.3% was detected within local Mass-type strains, supporting the hypothesis that multiple IBV serotypes may co-circulate in the same region simultaneously. Additionally, more conservation was observed amongst Mass serotypes versus QX-like serotypes, implying that vaccine use can influence the variability within the IBV population; this is deduced from the fact that the only live vaccine registered for use in South Africa at the time of the study was of Mass origin and no QX-like vaccines were available for use. This study offers the first published consolidation of IBV isolates from an area of South Africa and identifies variation within the IBV population of the broiler flock within the study area over a 2-year period. |
| first_indexed | 2024-12-11T00:30:33Z |
| format | Article |
| id | doaj.art-9440049cc53b4f7b8554679648532786 |
| institution | Directory Open Access Journal |
| issn | 0030-2465 2219-0635 |
| language | English |
| last_indexed | 2024-12-11T00:30:33Z |
| publishDate | 2014-09-01 |
| publisher | AOSIS |
| record_format | Article |
| series | Onderstepoort Journal of Veterinary Research |
| spelling | doaj.art-9440049cc53b4f7b85546796485327862022-12-22T01:27:22ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352014-09-01811e1e1010.4102/ojvr.v81i1.769458Two genotypes of infectious bronchitis virus are responsible for serological variation in KwaZulu-Natal poultry flocks prior to 2012Adrian D. Knoetze0Niloshni Moodley1Celia Abolnik2Rainbow Veterinary Laboratory, Rainbow Chicken Farms, South Africa; Department of Production Animal Studies, University of Pretoria, South AfricaRainbow Veterinary Laboratory, Rainbow Chicken FarmsDepartment of Production Animal Studies, University of PretoriaThis study describes the isolation, serotyping and genotyping of 54 infectious bronchitis virus (IBV) cases predominantly in KwaZulu-Natal and compared to several isolates from other South African provinces between 2011 and 2012 and several historic isolates. The results indicate the division of isolates into two different genotypes of IBV within the province, Massachusetts (Mass)-like and QX-like. The IBV Mass-like genotype was the most prevalent and was detected in 79% of the full spike protein S1 gene sequences. Variation up to 22.3% was detected within local Mass-type strains, supporting the hypothesis that multiple IBV serotypes may co-circulate in the same region simultaneously. Additionally, more conservation was observed amongst Mass serotypes versus QX-like serotypes, implying that vaccine use can influence the variability within the IBV population; this is deduced from the fact that the only live vaccine registered for use in South Africa at the time of the study was of Mass origin and no QX-like vaccines were available for use. This study offers the first published consolidation of IBV isolates from an area of South Africa and identifies variation within the IBV population of the broiler flock within the study area over a 2-year period.https://ojvr.org/index.php/ojvr/article/view/769infectious bronchitis viruspoultryS1 proteinMass serotypeQX-like serotypeRT-PCR |
| spellingShingle | Adrian D. Knoetze Niloshni Moodley Celia Abolnik Two genotypes of infectious bronchitis virus are responsible for serological variation in KwaZulu-Natal poultry flocks prior to 2012 Onderstepoort Journal of Veterinary Research infectious bronchitis virus poultry S1 protein Mass serotype QX-like serotype RT-PCR |
| title | Two genotypes of infectious bronchitis virus are responsible for serological variation in KwaZulu-Natal poultry flocks prior to 2012 |
| title_full | Two genotypes of infectious bronchitis virus are responsible for serological variation in KwaZulu-Natal poultry flocks prior to 2012 |
| title_fullStr | Two genotypes of infectious bronchitis virus are responsible for serological variation in KwaZulu-Natal poultry flocks prior to 2012 |
| title_full_unstemmed | Two genotypes of infectious bronchitis virus are responsible for serological variation in KwaZulu-Natal poultry flocks prior to 2012 |
| title_short | Two genotypes of infectious bronchitis virus are responsible for serological variation in KwaZulu-Natal poultry flocks prior to 2012 |
| title_sort | two genotypes of infectious bronchitis virus are responsible for serological variation in kwazulu natal poultry flocks prior to 2012 |
| topic | infectious bronchitis virus poultry S1 protein Mass serotype QX-like serotype RT-PCR |
| url | https://ojvr.org/index.php/ojvr/article/view/769 |
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