Classification and identification of Lactarius based on morphological characteristics of fruiting bodies and rDNA-ITS sequence analysis in Qiandongnan
ObjectiveIn order to clarify the taxonomic status of Lactarius in Qiandongnan of Guizhou, and provid the basis for the identification of wild fungi in foodborne wild mushroom poisoning.MethodsThe morphological characteristics of fruiting bodies and phylogenetic analysis of ribosomal DNA internal tra...
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2021-07-01
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| Series: | Zhongguo shipin weisheng zazhi |
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| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/20210409?st=article_issue |
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| author | WU Anzhong WEI Xiaobao ZENG Yi LIU Ying YANG Rui HUANG Junfei |
| author_facet | WU Anzhong WEI Xiaobao ZENG Yi LIU Ying YANG Rui HUANG Junfei |
| author_sort | WU Anzhong |
| collection | DOAJ |
| description | ObjectiveIn order to clarify the taxonomic status of Lactarius in Qiandongnan of Guizhou, and provid the basis for the identification of wild fungi in foodborne wild mushroom poisoning.MethodsThe morphological characteristics of fruiting bodies and phylogenetic analysis of ribosomal DNA internal transcribed spacer(rDNA-ITS) gene sequences were used for identification.ResultsIt was clear that there were 3 main kinds of Lactarius in the markets. The samples of indigo, amaranth and aurantius were identified as Lactarius vividus, Lactarius hatsudake and Lactarius subindigo based on morphological features. Then ITS gene sequence phylogenetic trees for the 34 fungi strains were divided into two branches, the Lactarius and other toxic fungi of the affinis species were distinguished. The TL1 and Lactarius subindig, TZ1 and Lactarius hatsudake, TH1 and Lactarius vividus were clustered into one group respectively, which were consistent with morphological identification results.ConclusionThe main species of Lactarius in Qiandongnan were the Lactarius hatsudake and Lactarius vividus, which were the same as another Lactarius in Guiyang. Among the Lactarius species, TL1 was identified as Lactarius subindigo, this was the first case reported in Guizhou. |
| first_indexed | 2024-04-11T04:47:56Z |
| format | Article |
| id | doaj.art-9442a319a91f4381b0cbfa8a9ab75fd3 |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-04-11T04:47:56Z |
| publishDate | 2021-07-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-9442a319a91f4381b0cbfa8a9ab75fd32022-12-27T09:23:55ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562021-07-01330443543910.13590/j.cjfh.2021.04.0091004-8456(2021)04-0435-05Classification and identification of Lactarius based on morphological characteristics of fruiting bodies and rDNA-ITS sequence analysis in QiandongnanWU Anzhong0WEI Xiaobao1ZENG Yi2LIU Ying3YANG Rui4HUANG Junfei5Guizhou Center for Disease Control and Prevention, Guizhou Guiyang 550004, ChinaGuizhou Center for Disease Control and Prevention, Guizhou Guiyang 550004, ChinaGuizhou Center for Disease Control and Prevention, Guizhou Guiyang 550004, ChinaGuizhou Center for Disease Control and Prevention, Guizhou Guiyang 550004, ChinaGuizhou Center for Disease Control and Prevention, Guizhou Guiyang 550004, ChinaGuizhou Center for Disease Control and Prevention, Guizhou Guiyang 550004, ChinaObjectiveIn order to clarify the taxonomic status of Lactarius in Qiandongnan of Guizhou, and provid the basis for the identification of wild fungi in foodborne wild mushroom poisoning.MethodsThe morphological characteristics of fruiting bodies and phylogenetic analysis of ribosomal DNA internal transcribed spacer(rDNA-ITS) gene sequences were used for identification.ResultsIt was clear that there were 3 main kinds of Lactarius in the markets. The samples of indigo, amaranth and aurantius were identified as Lactarius vividus, Lactarius hatsudake and Lactarius subindigo based on morphological features. Then ITS gene sequence phylogenetic trees for the 34 fungi strains were divided into two branches, the Lactarius and other toxic fungi of the affinis species were distinguished. The TL1 and Lactarius subindig, TZ1 and Lactarius hatsudake, TH1 and Lactarius vividus were clustered into one group respectively, which were consistent with morphological identification results.ConclusionThe main species of Lactarius in Qiandongnan were the Lactarius hatsudake and Lactarius vividus, which were the same as another Lactarius in Guiyang. Among the Lactarius species, TL1 was identified as Lactarius subindigo, this was the first case reported in Guizhou.http://www.zgspws.com/zgspwszz/article/abstract/20210409?st=article_issuelactariuslactarius vividuslactarius subindigolactarius hatsudakeclassification and identification |
| spellingShingle | WU Anzhong WEI Xiaobao ZENG Yi LIU Ying YANG Rui HUANG Junfei Classification and identification of Lactarius based on morphological characteristics of fruiting bodies and rDNA-ITS sequence analysis in Qiandongnan Zhongguo shipin weisheng zazhi lactarius lactarius vividus lactarius subindigo lactarius hatsudake classification and identification |
| title | Classification and identification of Lactarius based on morphological characteristics of fruiting bodies and rDNA-ITS sequence analysis in Qiandongnan |
| title_full | Classification and identification of Lactarius based on morphological characteristics of fruiting bodies and rDNA-ITS sequence analysis in Qiandongnan |
| title_fullStr | Classification and identification of Lactarius based on morphological characteristics of fruiting bodies and rDNA-ITS sequence analysis in Qiandongnan |
| title_full_unstemmed | Classification and identification of Lactarius based on morphological characteristics of fruiting bodies and rDNA-ITS sequence analysis in Qiandongnan |
| title_short | Classification and identification of Lactarius based on morphological characteristics of fruiting bodies and rDNA-ITS sequence analysis in Qiandongnan |
| title_sort | classification and identification of lactarius based on morphological characteristics of fruiting bodies and rdna its sequence analysis in qiandongnan |
| topic | lactarius lactarius vividus lactarius subindigo lactarius hatsudake classification and identification |
| url | http://www.zgspws.com/zgspwszz/article/abstract/20210409?st=article_issue |
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