Immunohistochemical Expression of Cyclin B1 in Epithelial Hyperplasia, Dysplasia and Oral Squamous Cell Carcinomas - A Comparative Study
Introduction: Cyclin B1 is important in the cell cycle progression from G2 to M phase. Cyclin B1 binds to CDC2, which then becomes dephosphorylated and gets relocated to the nucleus, ensuring the transition toward mitosis. Aim: Over expression of Cyclin B1, has been reported more recently in br...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2016-09-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/8563/19820_CE(EK)_F(AK)_PF1(P_AG_NE)_PFA(AK).pdf |
| Summary: | Introduction: Cyclin B1 is important in the cell cycle progression
from G2 to M phase. Cyclin B1 binds to CDC2, which then
becomes dephosphorylated and gets relocated to the nucleus,
ensuring the transition toward mitosis.
Aim: Over expression of Cyclin B1, has been reported more
recently in breast, colon, prostate, oral and esophageal carcinomas. Thus, the aim of the present study was to examine
the expression of Cyclin B1 in hyperplasia, dysplasia and Oral
Squamous Cell Carcinomas (OSCC).
Materials and Methods: A total of 64 histopathologically
diagnosed cases of epithelial hyperplasias, dysplastic
oral epithelium and OSCC were included in the study.
Immunohistochemical procedure was carried out using the
monoclonal mouse Cyclin B1 antibody (Clone V-152). The Cyclin
B1 positive tumor cells counted were expressed as percentage
of positive tumor cells. Nuclear and cytoplasmic labeling
index (n&cLI) were calculated. The results were tabulated and
statistically analyzed by Kruskal Wallis test- One Way ANOVA
and Mann Whitney U- test.
Results: Combined n&cLI was considered only in 28.57% of
epithelial hyperplasias, 40.7% of oral epithelial dysplasias and
72% of OSCC showed over expression of Cyclin B1 with p
value being 0.029. Cyclin B1 expression was not significantly
different between the grades of dysplasia, between the grades
of OSCC and between the marginal groups.
Conclusion: The present study demonstrates more than 50%
of the study group showing less than 20% of nuclear staining.
The importance of such variations within a type of lesion
requires further investigation, since Cyclin B1 has proved useful
in many studies from esophageal and laryngeal squamous cell
carcinoma as a prognostic indicator, an indicator of recurrence
and as an indicator for tumor sensitivity to radiotherapy. Further
studies are to be extended towards evaluating the role of Cyclin
B1 as a prognostic indicator. |
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| ISSN: | 2249-782X 0973-709X |