Elimination of the risks of colistin resistance gene (mcr-1) in livestock manure during composting
Since its discovery in Escherichia coli, the emergence and rapid spread of the plasmid-mediated colistin resistance gene mcr-1 have become a public health concern. Livestock manure is a potentially important reservoir of mcr-1 because colistin has been widely used in livestock production. Efforts ma...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-05-01
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| Series: | Environment International |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412018329180 |
| _version_ | 1811278668945686528 |
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| author | Yanzheng Gao Chao Lu Di Shen Juan Liu Zhao Ma Bing Yang Wanting Ling Michael Gatheru Waigi |
| author_facet | Yanzheng Gao Chao Lu Di Shen Juan Liu Zhao Ma Bing Yang Wanting Ling Michael Gatheru Waigi |
| author_sort | Yanzheng Gao |
| collection | DOAJ |
| description | Since its discovery in Escherichia coli, the emergence and rapid spread of the plasmid-mediated colistin resistance gene mcr-1 have become a public health concern. Livestock manure is a potentially important reservoir of mcr-1 because colistin has been widely used in livestock production. Efforts made to accurately quantify the prevalence of mcr-1 in livestock manure and the dynamic changes therein during thermophilic composting have been few and far between. In this study, mcr-1 in 51 collected samples from four kinds of livestock manures was detected and quantified. In total, 16 manure samples were found to be mcr-1 positive, with a detection frequency of 31% in 51 samples. The numbers of mcr-1 gene copies in 12 positive manure samples with a high prevalence of mcr-1 were 107–109 copies/g dry weight. During composting, >90% of mcr-1 in the manure was eliminated in 15 days at high temperature (44–65 °C), and mcr-1 was completely undetectable after 22 days. The reduction of mcr-1 following manure composting may be ascribed to the decreased number of potential mcr-1–harboring bacteria, Enterobacteriaceae and Pseudomonas. The results indicated that thermophilic composting effectively eliminated mcr-1 and inhibited its spread from livestock manure to the environment. Keywords: Mcr-1, QPCR, Thermophilic composting, Enterobacteriaceae, Pseudomonas |
| first_indexed | 2024-04-13T00:39:54Z |
| format | Article |
| id | doaj.art-94484791059a46228acda2c77a8a721a |
| institution | Directory Open Access Journal |
| issn | 0160-4120 |
| language | English |
| last_indexed | 2024-04-13T00:39:54Z |
| publishDate | 2019-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj.art-94484791059a46228acda2c77a8a721a2022-12-22T03:10:12ZengElsevierEnvironment International0160-41202019-05-011266168Elimination of the risks of colistin resistance gene (mcr-1) in livestock manure during compostingYanzheng Gao0Chao Lu1Di Shen2Juan Liu3Zhao Ma4Bing Yang5Wanting Ling6Michael Gatheru Waigi7Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR ChinaInstitute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR ChinaInstitute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR ChinaCorresponding author at: Weigang Road 1, Nanjing, Jiangsu 210095, PR China.; Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR ChinaInstitute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR ChinaInstitute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR ChinaInstitute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR ChinaInstitute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR ChinaSince its discovery in Escherichia coli, the emergence and rapid spread of the plasmid-mediated colistin resistance gene mcr-1 have become a public health concern. Livestock manure is a potentially important reservoir of mcr-1 because colistin has been widely used in livestock production. Efforts made to accurately quantify the prevalence of mcr-1 in livestock manure and the dynamic changes therein during thermophilic composting have been few and far between. In this study, mcr-1 in 51 collected samples from four kinds of livestock manures was detected and quantified. In total, 16 manure samples were found to be mcr-1 positive, with a detection frequency of 31% in 51 samples. The numbers of mcr-1 gene copies in 12 positive manure samples with a high prevalence of mcr-1 were 107–109 copies/g dry weight. During composting, >90% of mcr-1 in the manure was eliminated in 15 days at high temperature (44–65 °C), and mcr-1 was completely undetectable after 22 days. The reduction of mcr-1 following manure composting may be ascribed to the decreased number of potential mcr-1–harboring bacteria, Enterobacteriaceae and Pseudomonas. The results indicated that thermophilic composting effectively eliminated mcr-1 and inhibited its spread from livestock manure to the environment. Keywords: Mcr-1, QPCR, Thermophilic composting, Enterobacteriaceae, Pseudomonashttp://www.sciencedirect.com/science/article/pii/S0160412018329180 |
| spellingShingle | Yanzheng Gao Chao Lu Di Shen Juan Liu Zhao Ma Bing Yang Wanting Ling Michael Gatheru Waigi Elimination of the risks of colistin resistance gene (mcr-1) in livestock manure during composting Environment International |
| title | Elimination of the risks of colistin resistance gene (mcr-1) in livestock manure during composting |
| title_full | Elimination of the risks of colistin resistance gene (mcr-1) in livestock manure during composting |
| title_fullStr | Elimination of the risks of colistin resistance gene (mcr-1) in livestock manure during composting |
| title_full_unstemmed | Elimination of the risks of colistin resistance gene (mcr-1) in livestock manure during composting |
| title_short | Elimination of the risks of colistin resistance gene (mcr-1) in livestock manure during composting |
| title_sort | elimination of the risks of colistin resistance gene mcr 1 in livestock manure during composting |
| url | http://www.sciencedirect.com/science/article/pii/S0160412018329180 |
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