PROTEOMIC MASS-SPECTROMETRIC ANALYSIS OF FRESHLY ISOLATED ESCHERICHIA COLI STRAINS
Aim. Time-of-flight mass-spectrometric analysis of colon bacilli populations freshly isolated from humans for identification of proteome markers, that are characteristic for hemolytic and non-hemolytic strains, E.coli - associants of opportunistic bacteria. Materials and methods. 20 strains of monop...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Central Research Institute for Epidemiology
2015-06-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.crie.ru/jour/article/view/14077 |
| Summary: | Aim. Time-of-flight mass-spectrometric analysis of colon bacilli populations freshly isolated from humans for identification of proteome markers, that are characteristic for hemolytic and non-hemolytic strains, E.coli - associants of opportunistic bacteria. Materials and methods. 20 strains of monopopulation of hemolytic E.coli; 100 strains of monopopulation of non-hemolytic E.coli; 15 non-hemolytic strain, isolated from association with opportunistic bacteria. Protein profiling on MALDI-TOF MS Autoflex «Bruker Daltonik» mass-spectrometer. Results. Value within species for mass-charge peak with 100% intensity of 9000 Da could be a differential sign for isolation of hemolytic E.coli during mass-spectrometric biotyping. Conclusion. Proteomic characteristics of strains from various populations within E.coli are given. Taxon-specific markers for proteomic differentiation of E.coli populations are established. |
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| ISSN: | 0372-9311 2686-7613 |