Comparison of Methods for Quantifying Extracellular Vesicles of Gram-Negative Bacteria

Comparison of Methods for Quantifying Extracellular Vesicles of Gram-Negative Bacteria

There are a variety of methods employed by laboratories for quantifying extracellular vesicles isolated from bacteria. As a result, the ability to compare results across published studies can lead to questions regarding the suitability of methods and buffers for accurately quantifying these vesicles...

Full description

Bibliographic Details
Main Authors:	Chanel A. Mosby, Natalia Perez Devia, Melissa K. Jones
Format:	Article
Language:	English
Published:	MDPI AG 2023-10-01
Series:	International Journal of Molecular Sciences
Subjects:	bacterial extracellular vesicle outer membrane vesicles nanoparticle tracking analysis vesicle quantification Qubit NanoOrange
Online Access:	https://www.mdpi.com/1422-0067/24/20/15096

Similar Items

Bacterial extracellular vesicle applications in cancer immunotherapy
by: Kanika Suri, et al.
Published: (2023-04-01)

Changes in the Murine Microbiome and Bacterial Extracellular Vesicle Production in Response to Antibiotic Treatment and Norovirus Infection
by: Chanel A. Mosby, et al.
Published: (2023-12-01)

Roles of bacterial extracellular vesicles in systemic diseases
by: Yanzhen Wang, et al.
Published: (2023-09-01)

A new isolation method for bacterial extracellular vesicles providing greater purity and improved proteomic detection of vesicle proteins
by: Lena Hoang My Le, et al.
Published: (2023-05-01)

Differential temporal release and lipoprotein loading in B. thetaiotaomicron bacterial extracellular vesicles
by: Rokas Juodeikis, et al.
Published: (2024-01-01)

Salivary Outer Membrane Vesicles and DNA Methylation of Small Extracellular Vesicles as Biomarkers for Periodontal Status: A Pilot Study
by: Pingping Han, et al.
Published: (2021-02-01)

Emerging role of bacterial outer membrane vesicle in gastrointestinal tract
by: Cheng-mei Tian, et al.
Published: (2023-04-01)

Changes in lipid composition of host-derived extracellular vesicles following Salmonella infection
by: Lisa E. Emerson, et al.
Published: (2024-01-01)

Isolation and Quantification of Bacterial Membrane Vesicles for Quantitative Metabolic Studies Using Mammalian Cell Cultures
by: Marcel Kretschmer, et al.
Published: (2023-11-01)

Fluorescent Labeling of Helminth Extracellular Vesicles Using an In Vivo Whole Organism Approach
by: Anders T. Boysen, et al.
Published: (2020-07-01)

Comparison of interferometric light microscopy with nanoparticle tracking analysis for the study of extracellular vesicles and bacteriophages
by: Romain Sausset, et al.
Published: (2023-02-01)

Characterization of markers, functional properties, and microbiome composition in human gut-derived bacterial extracellular vesicles
by: Chih-Chi Li, et al.
Published: (2023-12-01)

Population Dynamics in the Biogenesis of Single-/Multi-Layered Membrane Vesicles Revealed by Encapsulated GFP-Monitoring Analysis
by: Sangho Koh, et al.
Published: (2023-09-01)

Extracellular Vesicles and Host–Pathogen Interactions: A Review of Inter-Kingdom Signaling by Small Noncoding RNA
by: Bruce A. Stanton
Published: (2021-06-01)

Mass‐produced gram‐negative bacterial outer membrane vesicles activate cancer antigen‐specific stem‐like CD8+ T cells which enables an effective combination immunotherapy with anti‐PD‐1
by: Solchan Won, et al.
Published: (2023-08-01)

From trash to treasure: the role of bacterial extracellular vesicles in gut health and disease
by: Desen Sun, et al.
Published: (2023-09-01)

Aptamer‐based extracellular vesicle isolation, analysis and therapeutics
by: Lin Zhu, et al.
Published: (2023-04-01)

Roles of extracellular vesicles in periodontal homeostasis and their therapeutic potential
by: Dao-Kun Deng, et al.
Published: (2022-12-01)

Membrane Vesicles as Drug Delivery Systems: Source, Preparation, Modification, Drug Loading, In Vivo Administration and Biodistribution, and Application in Various Diseases
by: Chenhan Sun, et al.
Published: (2023-07-01)

Outer membrane vesicles from bacteria: Role and potential value in the pathogenesis of chronic respiratory diseases
by: Fei Han, et al.
Published: (2022-12-01)

The Role of Extracellular Vesicles in Ischemic Stroke Severity
by: Angelica Carandina, et al.
Published: (2022-10-01)

Bacterial extracellular vesicles control murine norovirus infection through modulation of antiviral immune responses
by: Sutonuka Bhar, et al.
Published: (2022-08-01)

Extracellular vesicles of the probiotic bacteria E. coli O83 activate innate immunity and prevent allergy in mice
by: Anna Marlene Schmid, et al.
Published: (2023-10-01)

Bacterial outer membrane vesicles induce a transcriptional shift in arabidopsis towards immune system activation leading to suppression of pathogen growth in planta
by: Laura Chalupowicz, et al.
Published: (2023-01-01)

Extracellular Vesicles of <i>Pseudomonas</i>: Friends and Foes
by: Tania Henriquez, et al.
Published: (2023-04-01)

Both extracellular vesicles from helicobacter pylori-infected cells and helicobacter pylori outer membrane vesicles are involved in gastric/extragastric diseases
by: Chengyao Wang, et al.
Published: (2023-11-01)

Roles and therapeutic potential of different extracellular vesicle subtypes on traumatic brain injury
by: Xinlong Dong, et al.
Published: (2023-08-01)

Extracellular vesicle-mediated regulation of macrophage polarization in bacterial infections
by: Mingjuan Qu, et al.
Published: (2022-12-01)

Bacterial Membrane Vesicles in Pneumonia: From Mediators of Virulence to Innovative Vaccine Candidates
by: Felix Behrens, et al.
Published: (2021-04-01)

Extracellular vesicles: Emerging tools as therapeutic agent carriers
by: Shan Liu, et al.
Published: (2022-10-01)

Extracellular Vesicles: Current Analytical Techniques for Detection and Quantification
by: Esther Serrano-Pertierra, et al.
Published: (2020-05-01)

Detoxified synthetic bacterial membrane vesicles as a vaccine platform against bacteria and SARS-CoV-2
by: Kyong-Su Park, et al.
Published: (2023-05-01)

Extracellular Vesicle- and Extracellular Vesicle Mimetics-Based Drug Delivery Systems: New Perspectives, Challenges, and Clinical Developments
by: Prakash Gangadaran, et al.
Published: (2020-05-01)

miR-1227 Targets SEC23A to Regulate the Shedding of Large Extracellular Vesicles
by: Andrew Chin, et al.
Published: (2021-11-01)

Bacterial vesicles block viral replication in macrophages via TLR4-TRIF-axis
by: Jeff Bierwagen, et al.
Published: (2023-03-01)

Evaluation of EV Storage Buffer for Efficient Preservation of Engineered Extracellular Vesicles
by: Yuki Kawai-Harada, et al.
Published: (2023-08-01)

Extracellular Vesicles in the Fungi Kingdom
by: Marc Liebana-Jordan, et al.
Published: (2021-07-01)

Extracellular vesicles highlight many cases of photoreceptor degeneration
by: William J. Spencer
Published: (2023-05-01)

Role of bacterial infections in extracellular vesicles release and impact on immune response
by: Nicole Spencer, et al.
Published: (2021-04-01)

Exploring extracellular vesicles biogenesis in hypothalamic cells through a heavy isotope pulse/trace proteomic approach
by: Tan, Chee Fan, et al.
Published: (2020)