Serological methods for detection of the causative agent of tularemia and their evaluation
Aim. A comparative study of serological methods for the detection of the causative agent of tularemia and their evaluation. Materials and methods. We used experimental diagnostic kits and test systems for the production of serological methods: indirect hemagglutination reaction (RGA); the reaction i...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2019-09-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.elpub.ru/jour/article/view/427 |
| _version_ | 1811285321185230848 |
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| author | I. V. Zharnikova V. I. Efremenko T. V. Zharnikova S. A. Kurcheva S. M. Kalnoy D. V. Еfremenko A. A. Isakova A. V. Indenbom |
| author_facet | I. V. Zharnikova V. I. Efremenko T. V. Zharnikova S. A. Kurcheva S. M. Kalnoy D. V. Еfremenko A. A. Isakova A. V. Indenbom |
| author_sort | I. V. Zharnikova |
| collection | DOAJ |
| description | Aim. A comparative study of serological methods for the detection of the causative agent of tularemia and their evaluation. Materials and methods. We used experimental diagnostic kits and test systems for the production of serological methods: indirect hemagglutination reaction (RGA); the reaction immunofluorescence (RIF); enzyme immunoassay (ELISA) using traditional microplate; IFA after selective concentration of the pathogen of tularemia in magnoimmunosorbents (MIS); microgravimetric analysis (MGA) based on piezoresistors (SP) and surface plasmon resonance (SPR). The experiments were carried out with homologous strains of tularemia microbe (test strains) and with strains of heterologous microorganisms in model experiments on tap water contaminated with different concentrations of the pathogen. Results. The parameters of each diagnostic method are determined and evaluated according to the following indicators: sensitivity (when working with pure cultures (test strains), contaminated samples of large volumes), specificity, time of setting and taking into account the results, informativeness, determining the modes of setting and accounting. Conclusion. The above diagnostic methods have their advantages and disadvantages. Therefore, when choosing a method, the researcher should be guided by the goals pursued. So, for screening studies it is advisable to carry out the formulation of ELISA, RIF, RGA, in identifying the pathogen in large volumes and contaminated samples, the effective use of selective concentration on MIS followed by the formulation of ELISA, to identify small amounts of samples and take into account the reaction in real time, it is possible to use MGA and SPR. |
| first_indexed | 2024-04-13T02:42:15Z |
| format | Article |
| id | doaj.art-944fba1e10964a24b614d6b7c6b341a9 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-04-13T02:42:15Z |
| publishDate | 2019-09-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-944fba1e10964a24b614d6b7c6b341a92022-12-22T03:06:10ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132019-09-0104323810.36233/0372-9311-2019-4-32-38419Serological methods for detection of the causative agent of tularemia and their evaluationI. V. Zharnikova0V. I. Efremenko1T. V. Zharnikova2S. A. Kurcheva3S. M. Kalnoy4D. V. Еfremenko5A. A. Isakova6A. V. Indenbom7Stavropol State Institute for Plague ControlStavropol State Institute for Plague ControlStavropol State Institute for Plague ControlStavropol State Institute for Plague ControlStavropol State Institute for Plague ControlStavropol State Institute for Plague ControlFrumkin Institute of Physical Chemistry and ElectrochemistryFrumkin Institute of Physical Chemistry and Electrochemistry; Moscow Institute of Physics and Technology (State University)Aim. A comparative study of serological methods for the detection of the causative agent of tularemia and their evaluation. Materials and methods. We used experimental diagnostic kits and test systems for the production of serological methods: indirect hemagglutination reaction (RGA); the reaction immunofluorescence (RIF); enzyme immunoassay (ELISA) using traditional microplate; IFA after selective concentration of the pathogen of tularemia in magnoimmunosorbents (MIS); microgravimetric analysis (MGA) based on piezoresistors (SP) and surface plasmon resonance (SPR). The experiments were carried out with homologous strains of tularemia microbe (test strains) and with strains of heterologous microorganisms in model experiments on tap water contaminated with different concentrations of the pathogen. Results. The parameters of each diagnostic method are determined and evaluated according to the following indicators: sensitivity (when working with pure cultures (test strains), contaminated samples of large volumes), specificity, time of setting and taking into account the results, informativeness, determining the modes of setting and accounting. Conclusion. The above diagnostic methods have their advantages and disadvantages. Therefore, when choosing a method, the researcher should be guided by the goals pursued. So, for screening studies it is advisable to carry out the formulation of ELISA, RIF, RGA, in identifying the pathogen in large volumes and contaminated samples, the effective use of selective concentration on MIS followed by the formulation of ELISA, to identify small amounts of samples and take into account the reaction in real time, it is possible to use MGA and SPR.https://microbiol.elpub.ru/jour/article/view/427rgarifelisamismgapiezoresistorspr<i>francisella tularensis</i>antibodies |
| spellingShingle | I. V. Zharnikova V. I. Efremenko T. V. Zharnikova S. A. Kurcheva S. M. Kalnoy D. V. Еfremenko A. A. Isakova A. V. Indenbom Serological methods for detection of the causative agent of tularemia and their evaluation Журнал микробиологии, эпидемиологии и иммунобиологии rga rif elisa mis mga piezoresistor spr <i>francisella tularensis</i> antibodies |
| title | Serological methods for detection of the causative agent of tularemia and their evaluation |
| title_full | Serological methods for detection of the causative agent of tularemia and their evaluation |
| title_fullStr | Serological methods for detection of the causative agent of tularemia and their evaluation |
| title_full_unstemmed | Serological methods for detection of the causative agent of tularemia and their evaluation |
| title_short | Serological methods for detection of the causative agent of tularemia and their evaluation |
| title_sort | serological methods for detection of the causative agent of tularemia and their evaluation |
| topic | rga rif elisa mis mga piezoresistor spr <i>francisella tularensis</i> antibodies |
| url | https://microbiol.elpub.ru/jour/article/view/427 |
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