Effects of Spermidine on Cell Proliferation, Migration, and Inflammatory Response in Porcine Enterocytes
Background: Polyamines have been demonstrated to be beneficial to porcine intestinal development. Our previous study showed that putrescine mitigates intestinal atrophy in weanling piglets and suppresses inflammatory response in porcine intestinal epithelial cells, it is still unknown the role of sp...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IMR Press
2022-06-01
|
| Series: | Frontiers in Bioscience-Landmark |
| Subjects: | |
| Online Access: | https://www.imrpress.com/journal/FBL/27/6/10.31083/j.fbl2706194 |
| _version_ | 1811342780934389760 |
|---|---|
| author | Zi-xi Wei Long Cai Xue-mei Zhao Xian-ren Jiang Xi-long Li |
| author_facet | Zi-xi Wei Long Cai Xue-mei Zhao Xian-ren Jiang Xi-long Li |
| author_sort | Zi-xi Wei |
| collection | DOAJ |
| description | Background: Polyamines have been demonstrated to be beneficial to porcine intestinal development. Our previous study showed that putrescine mitigates intestinal atrophy in weanling piglets and suppresses inflammatory response in porcine intestinal epithelial cells, it is still unknown the role of spermidine in mediating putrescine function. Objective: The current study aimed to investigate the effect of spermidine on the proliferation, migration, and inflammatory response in porcine intestinal epithelial cells (IPEC-J2 cell line). Methods: The effects of spermidine on proliferation and migration of IPEC-J2 cells were measured. Difluoromethyl ornithine (DFMO) and diethylglyoxal bis (guanylhydrazone) (DEGBG) were used to block the production of putrescine and spermidine, respectively. A cell inflammation model was established with lipopolysaccharides (LPS) stimulation. Gene expression and protein abundance were determined by real-time quantitative PCR and western blotting, respectively. Result: Spermidine significantly enhanced cell proliferation in DFMO (or/and) DEGBG treated IPEC-J2 cells (p < 0.05). Pretreatment with putrescine restored cell growth inhibited by DFMO but did not prevent the decrease in cell proliferation caused by DEGBG (p > 0.05). Similarly, spermidine but not putrescine significantly elevated the rate of migration in DEGBG treated IPEC-J2 cells (p < 0.05). Spermidine deprivation by DEGBG dramatically enhanced mRNA abundance of pro-inflammatory cytokines IL-8, IL-6, and TNF-α (p < 0.05), and the addition of spermidine attenuated excessive expression of those inflammatory pro-inflammatory cytokines, moreover, spermidine but not putrescine suppressed the phosphorylation of NF-κB induced by DEGBG. Spermidine supplementation also significantly suppressed LPS-induced the expression of TNF-α. Conclusions: The present study highlights a novel insight that putrescine may be converted into spermidine to modulate cell proliferation, migration, and inflammatory response on porcine enterocytes. |
| first_indexed | 2024-04-13T19:16:59Z |
| format | Article |
| id | doaj.art-945145060da74c0faf976ba5a5b27e29 |
| institution | Directory Open Access Journal |
| issn | 2768-6701 |
| language | English |
| last_indexed | 2024-04-13T19:16:59Z |
| publishDate | 2022-06-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Frontiers in Bioscience-Landmark |
| spelling | doaj.art-945145060da74c0faf976ba5a5b27e292022-12-22T02:33:38ZengIMR PressFrontiers in Bioscience-Landmark2768-67012022-06-0127619410.31083/j.fbl2706194S2768-6701(22)00560-3Effects of Spermidine on Cell Proliferation, Migration, and Inflammatory Response in Porcine EnterocytesZi-xi Wei0Long Cai1Xue-mei Zhao2Xian-ren Jiang3Xi-long Li4Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, 100081 Beijing, ChinaLaboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, 100081 Beijing, ChinaLaboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, 100081 Beijing, ChinaLaboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, 100081 Beijing, ChinaLaboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, 100081 Beijing, ChinaBackground: Polyamines have been demonstrated to be beneficial to porcine intestinal development. Our previous study showed that putrescine mitigates intestinal atrophy in weanling piglets and suppresses inflammatory response in porcine intestinal epithelial cells, it is still unknown the role of spermidine in mediating putrescine function. Objective: The current study aimed to investigate the effect of spermidine on the proliferation, migration, and inflammatory response in porcine intestinal epithelial cells (IPEC-J2 cell line). Methods: The effects of spermidine on proliferation and migration of IPEC-J2 cells were measured. Difluoromethyl ornithine (DFMO) and diethylglyoxal bis (guanylhydrazone) (DEGBG) were used to block the production of putrescine and spermidine, respectively. A cell inflammation model was established with lipopolysaccharides (LPS) stimulation. Gene expression and protein abundance were determined by real-time quantitative PCR and western blotting, respectively. Result: Spermidine significantly enhanced cell proliferation in DFMO (or/and) DEGBG treated IPEC-J2 cells (p < 0.05). Pretreatment with putrescine restored cell growth inhibited by DFMO but did not prevent the decrease in cell proliferation caused by DEGBG (p > 0.05). Similarly, spermidine but not putrescine significantly elevated the rate of migration in DEGBG treated IPEC-J2 cells (p < 0.05). Spermidine deprivation by DEGBG dramatically enhanced mRNA abundance of pro-inflammatory cytokines IL-8, IL-6, and TNF-α (p < 0.05), and the addition of spermidine attenuated excessive expression of those inflammatory pro-inflammatory cytokines, moreover, spermidine but not putrescine suppressed the phosphorylation of NF-κB induced by DEGBG. Spermidine supplementation also significantly suppressed LPS-induced the expression of TNF-α. Conclusions: The present study highlights a novel insight that putrescine may be converted into spermidine to modulate cell proliferation, migration, and inflammatory response on porcine enterocytes.https://www.imrpress.com/journal/FBL/27/6/10.31083/j.fbl2706194spermidineputrescinecell proliferationcell migrationinflammatory response |
| spellingShingle | Zi-xi Wei Long Cai Xue-mei Zhao Xian-ren Jiang Xi-long Li Effects of Spermidine on Cell Proliferation, Migration, and Inflammatory Response in Porcine Enterocytes Frontiers in Bioscience-Landmark spermidine putrescine cell proliferation cell migration inflammatory response |
| title | Effects of Spermidine on Cell Proliferation, Migration, and Inflammatory Response in Porcine Enterocytes |
| title_full | Effects of Spermidine on Cell Proliferation, Migration, and Inflammatory Response in Porcine Enterocytes |
| title_fullStr | Effects of Spermidine on Cell Proliferation, Migration, and Inflammatory Response in Porcine Enterocytes |
| title_full_unstemmed | Effects of Spermidine on Cell Proliferation, Migration, and Inflammatory Response in Porcine Enterocytes |
| title_short | Effects of Spermidine on Cell Proliferation, Migration, and Inflammatory Response in Porcine Enterocytes |
| title_sort | effects of spermidine on cell proliferation migration and inflammatory response in porcine enterocytes |
| topic | spermidine putrescine cell proliferation cell migration inflammatory response |
| url | https://www.imrpress.com/journal/FBL/27/6/10.31083/j.fbl2706194 |
| work_keys_str_mv | AT zixiwei effectsofspermidineoncellproliferationmigrationandinflammatoryresponseinporcineenterocytes AT longcai effectsofspermidineoncellproliferationmigrationandinflammatoryresponseinporcineenterocytes AT xuemeizhao effectsofspermidineoncellproliferationmigrationandinflammatoryresponseinporcineenterocytes AT xianrenjiang effectsofspermidineoncellproliferationmigrationandinflammatoryresponseinporcineenterocytes AT xilongli effectsofspermidineoncellproliferationmigrationandinflammatoryresponseinporcineenterocytes |