Litter Management Strategies and Their Impact on the Environmental and Respiratory Microbiome Might Influence Health in Poultry
Aerial and respiratory tract-associated bacterial diversity has been scarcely studied in broiler production systems. This study examined the relationship between the environmental air and birds’ respiratory microbiome, considering a longitudinal sampling. Total viable bacteria and coliforms in the a...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/5/878 |
| _version_ | 1797497719474880512 |
|---|---|
| author | Dinka Ivulic Ramon Rossello-Mora Tomeu Viver David A. Montero Sonia Vidal Francisco Aspee Héctor Hidalgo Roberto Vidal |
| author_facet | Dinka Ivulic Ramon Rossello-Mora Tomeu Viver David A. Montero Sonia Vidal Francisco Aspee Héctor Hidalgo Roberto Vidal |
| author_sort | Dinka Ivulic |
| collection | DOAJ |
| description | Aerial and respiratory tract-associated bacterial diversity has been scarcely studied in broiler production systems. This study examined the relationship between the environmental air and birds’ respiratory microbiome, considering a longitudinal sampling. Total viable bacteria and coliforms in the air were quantified, and the 16S rRNA gene was sequenced from tracheal and air samples obtained through a novelty protocol. Air results showed a decrease in coliforms over time. However, at week 3, we reported an increase in coliforms (from 143 to 474 CFUc/m<sup>3</sup>) associated with litter management. Additionally, 16S rRNA gene results indicated a distinctive air microbial community, associated primarily with <i>Bacillota</i> phylum particularly of the <i>Bacilli</i> class (>58%), under all conditions. Tracheal results indicated a predominance of <i>Escherichia coli/Shigella</i> at the beginning of the productive cycle, shifting toward the middle and end of the cycle to <i>Gallibacterium</i>. However, at week 3, the dominance of <i>Escherichia coli/Shigella</i> (>99.5%) associated with litter aeration by tumbling stood out. Tracheal and air samples displayed a statistically different community structure, but shared differentially abundant features through time: <i>Enterococcus</i>, <i>Gallibacterium</i>, and <i>Romboutsia ilealis</i>. These results indicate the impact of production management protocols on the birds’ respiratory system that should be considered a breakpoint in poultry farm health. |
| first_indexed | 2024-03-10T03:23:16Z |
| format | Article |
| id | doaj.art-94569edf42df436b9873f8c76162ea4a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T03:23:16Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-94569edf42df436b9873f8c76162ea4a2023-11-23T12:14:21ZengMDPI AGMicroorganisms2076-26072022-04-0110587810.3390/microorganisms10050878Litter Management Strategies and Their Impact on the Environmental and Respiratory Microbiome Might Influence Health in PoultryDinka Ivulic0Ramon Rossello-Mora1Tomeu Viver2David A. Montero3Sonia Vidal4Francisco Aspee5Héctor Hidalgo6Roberto Vidal7Programa de Doctorado en Ciencias Silvoagropecuarias y Veterinarias, Campus Sur Universidad de Chile, Santa Rosa 11315, La Pintana, Santiago 8820808, ChileMarine Microbiology Group, Department of Animal and Microbial Diversity, IMEDEA (CSIC-UIB), 07190 Esporles, Illes Balears, SpainMarine Microbiology Group, Department of Animal and Microbial Diversity, IMEDEA (CSIC-UIB), 07190 Esporles, Illes Balears, SpainPrograma de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago 8380453, ChileLaboratory of Veterinary Vaccines, Department of Animal Biology, Faculty of Veterinary and Animal Science, Universidad de Chile, Santiago 8820808, ChileAgrícola Ariztía Ltda., Melipilla 9580752, ChileLaboratory of Avian Pathology, Faculty of Veterinary and Animal Sciences, Universidad de Chile, Santiago 8820808, ChilePrograma de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago 8380453, ChileAerial and respiratory tract-associated bacterial diversity has been scarcely studied in broiler production systems. This study examined the relationship between the environmental air and birds’ respiratory microbiome, considering a longitudinal sampling. Total viable bacteria and coliforms in the air were quantified, and the 16S rRNA gene was sequenced from tracheal and air samples obtained through a novelty protocol. Air results showed a decrease in coliforms over time. However, at week 3, we reported an increase in coliforms (from 143 to 474 CFUc/m<sup>3</sup>) associated with litter management. Additionally, 16S rRNA gene results indicated a distinctive air microbial community, associated primarily with <i>Bacillota</i> phylum particularly of the <i>Bacilli</i> class (>58%), under all conditions. Tracheal results indicated a predominance of <i>Escherichia coli/Shigella</i> at the beginning of the productive cycle, shifting toward the middle and end of the cycle to <i>Gallibacterium</i>. However, at week 3, the dominance of <i>Escherichia coli/Shigella</i> (>99.5%) associated with litter aeration by tumbling stood out. Tracheal and air samples displayed a statistically different community structure, but shared differentially abundant features through time: <i>Enterococcus</i>, <i>Gallibacterium</i>, and <i>Romboutsia ilealis</i>. These results indicate the impact of production management protocols on the birds’ respiratory system that should be considered a breakpoint in poultry farm health.https://www.mdpi.com/2076-2607/10/5/878microbiomebroilerstrachealairavianlitter |
| spellingShingle | Dinka Ivulic Ramon Rossello-Mora Tomeu Viver David A. Montero Sonia Vidal Francisco Aspee Héctor Hidalgo Roberto Vidal Litter Management Strategies and Their Impact on the Environmental and Respiratory Microbiome Might Influence Health in Poultry Microorganisms microbiome broilers tracheal air avian litter |
| title | Litter Management Strategies and Their Impact on the Environmental and Respiratory Microbiome Might Influence Health in Poultry |
| title_full | Litter Management Strategies and Their Impact on the Environmental and Respiratory Microbiome Might Influence Health in Poultry |
| title_fullStr | Litter Management Strategies and Their Impact on the Environmental and Respiratory Microbiome Might Influence Health in Poultry |
| title_full_unstemmed | Litter Management Strategies and Their Impact on the Environmental and Respiratory Microbiome Might Influence Health in Poultry |
| title_short | Litter Management Strategies and Their Impact on the Environmental and Respiratory Microbiome Might Influence Health in Poultry |
| title_sort | litter management strategies and their impact on the environmental and respiratory microbiome might influence health in poultry |
| topic | microbiome broilers tracheal air avian litter |
| url | https://www.mdpi.com/2076-2607/10/5/878 |
| work_keys_str_mv | AT dinkaivulic littermanagementstrategiesandtheirimpactontheenvironmentalandrespiratorymicrobiomemightinfluencehealthinpoultry AT ramonrossellomora littermanagementstrategiesandtheirimpactontheenvironmentalandrespiratorymicrobiomemightinfluencehealthinpoultry AT tomeuviver littermanagementstrategiesandtheirimpactontheenvironmentalandrespiratorymicrobiomemightinfluencehealthinpoultry AT davidamontero littermanagementstrategiesandtheirimpactontheenvironmentalandrespiratorymicrobiomemightinfluencehealthinpoultry AT soniavidal littermanagementstrategiesandtheirimpactontheenvironmentalandrespiratorymicrobiomemightinfluencehealthinpoultry AT franciscoaspee littermanagementstrategiesandtheirimpactontheenvironmentalandrespiratorymicrobiomemightinfluencehealthinpoultry AT hectorhidalgo littermanagementstrategiesandtheirimpactontheenvironmentalandrespiratorymicrobiomemightinfluencehealthinpoultry AT robertovidal littermanagementstrategiesandtheirimpactontheenvironmentalandrespiratorymicrobiomemightinfluencehealthinpoultry |