Arrangement of the Autonomic Nerves Around the Pulmonary Vein-Left Atrial Junctions —Histologic and Immunohistochemical Analyses—
Introduction: Imbalanced autonomic activity in the area of the pulmonary veins (PVs) can result in spontaneous atrial fibrillation (AF). Histologic characteristics of the PV sleeve musculature and associated autonomic nerve are not fully understood. We investigated the arrangement of autonomic nerve...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2006-01-01
|
| Series: | Journal of Arrhythmia |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1880427606800335 |
| _version_ | 1828375943531462656 |
|---|---|
| author | Taka-aki Matsuyama, MD Shin Inoue, MD Youichi Kobayashi, MD Mutsuki Makino, MD Tetsuo Sakai, MD Tsukasa Saito, MD Taku Asano, MD Kaoru Tanno, MD Takashi Katagiri, MD Hidekazu Ota, MD |
| author_facet | Taka-aki Matsuyama, MD Shin Inoue, MD Youichi Kobayashi, MD Mutsuki Makino, MD Tetsuo Sakai, MD Tsukasa Saito, MD Taku Asano, MD Kaoru Tanno, MD Takashi Katagiri, MD Hidekazu Ota, MD |
| author_sort | Taka-aki Matsuyama, MD |
| collection | DOAJ |
| description | Introduction: Imbalanced autonomic activity in the area of the pulmonary veins (PVs) can result in spontaneous atrial fibrillation (AF). Histologic characteristics of the PV sleeve musculature and associated autonomic nerve are not fully understood. We investigated the arrangement of autonomic nerve fibers around PV-left atrium (LA) junctional musculature.
Methods: Thirteen autopsied adult hearts (9 men and 4 women; mean age at death, 66.2 years were studied. The atria were removed from each heart, along with all PV stalks, and cut longitudinally to each PV myocardial sleeve. After treatment with azan-Mallory stain and immunohistochemical staining for S-100 and tyrosine hydroxylase (TH), autonomic nerve distribution was assessed by counting the numbers of TH-positive (adrenergic and -negative (non-adrenergic) fibers within S-100-positive fibers (>50 mm in diameter) in the anterior, posterior, and septal junctions.
Results: TH-positive adrenergic fibers, consisting of sympathetic nerves, were most predominant in the anterior and septal junctions. In the anterior junction, these fibers were packed tightly among myocardial sleeve fascicles. In the posterior junction, the numbers of adrenergic and non-adrenergic fibers were fewer. In the septal junction, the number of THnegative non-adrenergic fibers (predominantly parasympathetic nerves) was greater, concomitant diffuse ganglionic nodule distribution in the interatrial fat pad.
Conclusions: In each PV-LA junction, autonomic nerves were localized on the anterior and septal walls. Heterogeneous distribution of TH-positive and TH-negative fibers and ganglion nodules around each PV opening appears to represent the major histologic characteristic in these areas. |
| first_indexed | 2024-04-14T07:53:56Z |
| format | Article |
| id | doaj.art-9456d44b5bf5466c8ecc7650628c87d6 |
| institution | Directory Open Access Journal |
| issn | 1880-4276 |
| language | English |
| last_indexed | 2024-04-14T07:53:56Z |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Arrhythmia |
| spelling | doaj.art-9456d44b5bf5466c8ecc7650628c87d62022-12-22T02:05:07ZengWileyJournal of Arrhythmia1880-42762006-01-0122423424110.1016/S1880-4276(06)80033-5Arrangement of the Autonomic Nerves Around the Pulmonary Vein-Left Atrial Junctions —Histologic and Immunohistochemical Analyses—Taka-aki Matsuyama, MD0Shin Inoue, MD1Youichi Kobayashi, MD2Mutsuki Makino, MD3Tetsuo Sakai, MD4Tsukasa Saito, MD5Taku Asano, MD6Kaoru Tanno, MD7Takashi Katagiri, MD8Hidekazu Ota, MD9Second Department of Pathology, Showa University School of MedicineDepartment of Internal Medicine, Showa University Dental HospitalThird Department of Internal Medicine, Showa University School of Medicine, Tokyo, JapanSecond Department of Pathology, Showa University School of MedicineThird Department of Internal Medicine, Showa University School of Medicine, Tokyo, JapanThird Department of Internal Medicine, Showa University School of Medicine, Tokyo, JapanThird Department of Internal Medicine, Showa University School of Medicine, Tokyo, JapanThird Department of Internal Medicine, Showa University School of Medicine, Tokyo, JapanThird Department of Internal Medicine, Showa University School of Medicine, Tokyo, JapanSecond Department of Pathology, Showa University School of MedicineIntroduction: Imbalanced autonomic activity in the area of the pulmonary veins (PVs) can result in spontaneous atrial fibrillation (AF). Histologic characteristics of the PV sleeve musculature and associated autonomic nerve are not fully understood. We investigated the arrangement of autonomic nerve fibers around PV-left atrium (LA) junctional musculature. Methods: Thirteen autopsied adult hearts (9 men and 4 women; mean age at death, 66.2 years were studied. The atria were removed from each heart, along with all PV stalks, and cut longitudinally to each PV myocardial sleeve. After treatment with azan-Mallory stain and immunohistochemical staining for S-100 and tyrosine hydroxylase (TH), autonomic nerve distribution was assessed by counting the numbers of TH-positive (adrenergic and -negative (non-adrenergic) fibers within S-100-positive fibers (>50 mm in diameter) in the anterior, posterior, and septal junctions. Results: TH-positive adrenergic fibers, consisting of sympathetic nerves, were most predominant in the anterior and septal junctions. In the anterior junction, these fibers were packed tightly among myocardial sleeve fascicles. In the posterior junction, the numbers of adrenergic and non-adrenergic fibers were fewer. In the septal junction, the number of THnegative non-adrenergic fibers (predominantly parasympathetic nerves) was greater, concomitant diffuse ganglionic nodule distribution in the interatrial fat pad. Conclusions: In each PV-LA junction, autonomic nerves were localized on the anterior and septal walls. Heterogeneous distribution of TH-positive and TH-negative fibers and ganglion nodules around each PV opening appears to represent the major histologic characteristic in these areas.http://www.sciencedirect.com/science/article/pii/S1880427606800335Atrial fibrillationHistologyMyocardial sleeveNerve distributionTyrosine hydroxylase |
| spellingShingle | Taka-aki Matsuyama, MD Shin Inoue, MD Youichi Kobayashi, MD Mutsuki Makino, MD Tetsuo Sakai, MD Tsukasa Saito, MD Taku Asano, MD Kaoru Tanno, MD Takashi Katagiri, MD Hidekazu Ota, MD Arrangement of the Autonomic Nerves Around the Pulmonary Vein-Left Atrial Junctions —Histologic and Immunohistochemical Analyses— Journal of Arrhythmia Atrial fibrillation Histology Myocardial sleeve Nerve distribution Tyrosine hydroxylase |
| title | Arrangement of the Autonomic Nerves Around the Pulmonary Vein-Left Atrial Junctions —Histologic and Immunohistochemical Analyses— |
| title_full | Arrangement of the Autonomic Nerves Around the Pulmonary Vein-Left Atrial Junctions —Histologic and Immunohistochemical Analyses— |
| title_fullStr | Arrangement of the Autonomic Nerves Around the Pulmonary Vein-Left Atrial Junctions —Histologic and Immunohistochemical Analyses— |
| title_full_unstemmed | Arrangement of the Autonomic Nerves Around the Pulmonary Vein-Left Atrial Junctions —Histologic and Immunohistochemical Analyses— |
| title_short | Arrangement of the Autonomic Nerves Around the Pulmonary Vein-Left Atrial Junctions —Histologic and Immunohistochemical Analyses— |
| title_sort | arrangement of the autonomic nerves around the pulmonary vein left atrial junctions histologic and immunohistochemical analyses |
| topic | Atrial fibrillation Histology Myocardial sleeve Nerve distribution Tyrosine hydroxylase |
| url | http://www.sciencedirect.com/science/article/pii/S1880427606800335 |
| work_keys_str_mv | AT takaakimatsuyamamd arrangementoftheautonomicnervesaroundthepulmonaryveinleftatrialjunctionshistologicandimmunohistochemicalanalyses AT shininouemd arrangementoftheautonomicnervesaroundthepulmonaryveinleftatrialjunctionshistologicandimmunohistochemicalanalyses AT youichikobayashimd arrangementoftheautonomicnervesaroundthepulmonaryveinleftatrialjunctionshistologicandimmunohistochemicalanalyses AT mutsukimakinomd arrangementoftheautonomicnervesaroundthepulmonaryveinleftatrialjunctionshistologicandimmunohistochemicalanalyses AT tetsuosakaimd arrangementoftheautonomicnervesaroundthepulmonaryveinleftatrialjunctionshistologicandimmunohistochemicalanalyses AT tsukasasaitomd arrangementoftheautonomicnervesaroundthepulmonaryveinleftatrialjunctionshistologicandimmunohistochemicalanalyses AT takuasanomd arrangementoftheautonomicnervesaroundthepulmonaryveinleftatrialjunctionshistologicandimmunohistochemicalanalyses AT kaorutannomd arrangementoftheautonomicnervesaroundthepulmonaryveinleftatrialjunctionshistologicandimmunohistochemicalanalyses AT takashikatagirimd arrangementoftheautonomicnervesaroundthepulmonaryveinleftatrialjunctionshistologicandimmunohistochemicalanalyses AT hidekazuotamd arrangementoftheautonomicnervesaroundthepulmonaryveinleftatrialjunctionshistologicandimmunohistochemicalanalyses |