Wound healing after excision of subcutaneous tumors treated with near‐infrared photoimmunotherapy
Abstract Near‐infrared photoimmunotherapy (NIR‐PIT) is a novel cancer therapy that employs a combination of infrared light and tumor‐targeted monoclonal antibody‐photoabsorber conjugates to cause both direct tumor necrosis and immunogenic cell death. NIR‐PIT may have potential in the perioperative s...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-08-01
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| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.3247 |
| _version_ | 1797387131227734016 |
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| author | Adrian Rosenberg Fuyuki Inagaki Takuya Kato Ryuhei Okada Hiroaki Wakiyama Aki Furusawa Peter L. Choyke Hisataka Kobayashi |
| author_facet | Adrian Rosenberg Fuyuki Inagaki Takuya Kato Ryuhei Okada Hiroaki Wakiyama Aki Furusawa Peter L. Choyke Hisataka Kobayashi |
| author_sort | Adrian Rosenberg |
| collection | DOAJ |
| description | Abstract Near‐infrared photoimmunotherapy (NIR‐PIT) is a novel cancer therapy that employs a combination of infrared light and tumor‐targeted monoclonal antibody‐photoabsorber conjugates to cause both direct tumor necrosis and immunogenic cell death. NIR‐PIT may have potential in the perioperative setting before surgery, and therefore it is important to know the effect of NIR‐PIT on wound healing. Fifty mice were implanted with subcutaneous xenografts of N87 human gastric cancer cells, and tumors were excised after reaching a predetermined size. After excision, 30 mice were split into three groups: Controls, NIR‐PIT 1 day prior to surgery and NIR‐PIT 3 days prior to surgery. The quantity of reactive oxygen species (ROS) in each wound was measured on Postoperative Days 2 and 4, and mice were monitored weekly for 4 weeks for evidence of local tumor recurrence as well as clinical evidence of wound healing complications (eg, dehiscence, infection). The remaining 20 mice (10 controls, 10 treated with NIR‐PIT 1 day prior to surgery) were sacrificed on either Postoperative Day 7 or 14, the skin around wounds were excised, and tensile strength was measured with a digital force gauge. There were no significant differences between treatment and control groups with respect to wound ROS levels, wound tensile strength, local tumor recurrence, or postoperative complication rates (P > .05). In conclusion, neoadjuvant (pre‐operative) NIR‐PIT shows no evidence of adverse wound healing effects, and it is likely a safe adjunctive treatment to surgery. Postoperative use of NIR‐PIT merits investigation. |
| first_indexed | 2024-03-08T22:20:26Z |
| format | Article |
| id | doaj.art-9458232a636a4ba8b0984da1c8fbd79d |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-03-08T22:20:26Z |
| publishDate | 2020-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-9458232a636a4ba8b0984da1c8fbd79d2023-12-18T12:56:30ZengWileyCancer Medicine2045-76342020-08-019165932593910.1002/cam4.3247Wound healing after excision of subcutaneous tumors treated with near‐infrared photoimmunotherapyAdrian Rosenberg0Fuyuki Inagaki1Takuya Kato2Ryuhei Okada3Hiroaki Wakiyama4Aki Furusawa5Peter L. Choyke6Hisataka Kobayashi7Molecular Imaging Program Center for Cancer Research National Cancer InstituteNational Institutes of Health Bethesda MD USAMolecular Imaging Program Center for Cancer Research National Cancer InstituteNational Institutes of Health Bethesda MD USAMolecular Imaging Program Center for Cancer Research National Cancer InstituteNational Institutes of Health Bethesda MD USAMolecular Imaging Program Center for Cancer Research National Cancer InstituteNational Institutes of Health Bethesda MD USAMolecular Imaging Program Center for Cancer Research National Cancer InstituteNational Institutes of Health Bethesda MD USAMolecular Imaging Program Center for Cancer Research National Cancer InstituteNational Institutes of Health Bethesda MD USAMolecular Imaging Program Center for Cancer Research National Cancer InstituteNational Institutes of Health Bethesda MD USAMolecular Imaging Program Center for Cancer Research National Cancer InstituteNational Institutes of Health Bethesda MD USAAbstract Near‐infrared photoimmunotherapy (NIR‐PIT) is a novel cancer therapy that employs a combination of infrared light and tumor‐targeted monoclonal antibody‐photoabsorber conjugates to cause both direct tumor necrosis and immunogenic cell death. NIR‐PIT may have potential in the perioperative setting before surgery, and therefore it is important to know the effect of NIR‐PIT on wound healing. Fifty mice were implanted with subcutaneous xenografts of N87 human gastric cancer cells, and tumors were excised after reaching a predetermined size. After excision, 30 mice were split into three groups: Controls, NIR‐PIT 1 day prior to surgery and NIR‐PIT 3 days prior to surgery. The quantity of reactive oxygen species (ROS) in each wound was measured on Postoperative Days 2 and 4, and mice were monitored weekly for 4 weeks for evidence of local tumor recurrence as well as clinical evidence of wound healing complications (eg, dehiscence, infection). The remaining 20 mice (10 controls, 10 treated with NIR‐PIT 1 day prior to surgery) were sacrificed on either Postoperative Day 7 or 14, the skin around wounds were excised, and tensile strength was measured with a digital force gauge. There were no significant differences between treatment and control groups with respect to wound ROS levels, wound tensile strength, local tumor recurrence, or postoperative complication rates (P > .05). In conclusion, neoadjuvant (pre‐operative) NIR‐PIT shows no evidence of adverse wound healing effects, and it is likely a safe adjunctive treatment to surgery. Postoperative use of NIR‐PIT merits investigation.https://doi.org/10.1002/cam4.3247near‐infrared photoimmunotherapyneoadjuvantreactive oxygen speciessurgerywound healing |
| spellingShingle | Adrian Rosenberg Fuyuki Inagaki Takuya Kato Ryuhei Okada Hiroaki Wakiyama Aki Furusawa Peter L. Choyke Hisataka Kobayashi Wound healing after excision of subcutaneous tumors treated with near‐infrared photoimmunotherapy Cancer Medicine near‐infrared photoimmunotherapy neoadjuvant reactive oxygen species surgery wound healing |
| title | Wound healing after excision of subcutaneous tumors treated with near‐infrared photoimmunotherapy |
| title_full | Wound healing after excision of subcutaneous tumors treated with near‐infrared photoimmunotherapy |
| title_fullStr | Wound healing after excision of subcutaneous tumors treated with near‐infrared photoimmunotherapy |
| title_full_unstemmed | Wound healing after excision of subcutaneous tumors treated with near‐infrared photoimmunotherapy |
| title_short | Wound healing after excision of subcutaneous tumors treated with near‐infrared photoimmunotherapy |
| title_sort | wound healing after excision of subcutaneous tumors treated with near infrared photoimmunotherapy |
| topic | near‐infrared photoimmunotherapy neoadjuvant reactive oxygen species surgery wound healing |
| url | https://doi.org/10.1002/cam4.3247 |
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