<i>Boletus edulis</i> Extract—A New Modulator of Dysbiotic Microbiota
The regular administration of antibiotics is a public concern due to the prejudices of large population groups and the high frequency with which antimicrobial products are prescribed. The current study aimed to evaluate the in vitro effect of a new extract from <i>Boletus edulis</i> (BEE...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/13/7/1481 |
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| author | Ionela Avram Diana Pelinescu Florentina Gatea Robertina Ionescu Alexandru Barcan Razvan Rosca Anca Zanfirescu Emanuel Vamanu |
| author_facet | Ionela Avram Diana Pelinescu Florentina Gatea Robertina Ionescu Alexandru Barcan Razvan Rosca Anca Zanfirescu Emanuel Vamanu |
| author_sort | Ionela Avram |
| collection | DOAJ |
| description | The regular administration of antibiotics is a public concern due to the prejudices of large population groups and the high frequency with which antimicrobial products are prescribed. The current study aimed to evaluate the in vitro effect of a new extract from <i>Boletus edulis</i> (BEE) on the human microbiota. One of the disadvantages of this extensive use is the disruption of the human microbiota, leading to potential negative health consequences. The in vitro evaluation of BEE consisted in determining its cytotoxicity, influence on the concentration of four types of cytokines (IL-6, IL-10, IL-1β, TNFα), and capacity to modulate the human microbiota after administering antibiotics. The latter was assessed by microbiome analysis and the evaluation of short-chain fatty acid synthesis (SCFAs). Simultaneously, the content of total polyphenols, the antioxidant capacity, and the compositional analysis of the extract (individual polyphenols composition) were determined. The results showed that BEE modulates the microbial pattern and reduces inflammatory progression. The data demonstrated antioxidant properties correlated with the increase in synthesizing some biomarkers, such as SCFAs, which mitigated antibiotic-induced dysbiosis without using probiotic products. |
| first_indexed | 2024-03-11T00:54:56Z |
| format | Article |
| id | doaj.art-945947f2c7e7498a9052ffdd033c2193 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T00:54:56Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-945947f2c7e7498a9052ffdd033c21932023-11-18T20:09:05ZengMDPI AGLife2075-17292023-06-01137148110.3390/life13071481<i>Boletus edulis</i> Extract—A New Modulator of Dysbiotic MicrobiotaIonela Avram0Diana Pelinescu1Florentina Gatea2Robertina Ionescu3Alexandru Barcan4Razvan Rosca5Anca Zanfirescu6Emanuel Vamanu7Department of Genetics, University of Bucharest, 36-46 Bd. M. Kogalniceanu, 5th District, 050107 Bucharest, RomaniaDepartment of Genetics, University of Bucharest, 36-46 Bd. M. Kogalniceanu, 5th District, 050107 Bucharest, RomaniaCentre of Bioanalysis, National Institute for Biological Sciences, 296 Spl. Independentei, 060031 Bucharest, RomaniaDepartment of Genetics, University of Bucharest, 36-46 Bd. M. Kogalniceanu, 5th District, 050107 Bucharest, RomaniaFaculty of Biotechnology, University of Agricultural Sciences and Veterinary Medicine, 011464 Bucharest, RomaniaAnoom Laboratories SRL, București, 28 Vintila Mihaileanu Sector 1, 024023 Bucharest, RomaniaFaculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, RomaniaFaculty of Biotechnology, University of Agricultural Sciences and Veterinary Medicine, 011464 Bucharest, RomaniaThe regular administration of antibiotics is a public concern due to the prejudices of large population groups and the high frequency with which antimicrobial products are prescribed. The current study aimed to evaluate the in vitro effect of a new extract from <i>Boletus edulis</i> (BEE) on the human microbiota. One of the disadvantages of this extensive use is the disruption of the human microbiota, leading to potential negative health consequences. The in vitro evaluation of BEE consisted in determining its cytotoxicity, influence on the concentration of four types of cytokines (IL-6, IL-10, IL-1β, TNFα), and capacity to modulate the human microbiota after administering antibiotics. The latter was assessed by microbiome analysis and the evaluation of short-chain fatty acid synthesis (SCFAs). Simultaneously, the content of total polyphenols, the antioxidant capacity, and the compositional analysis of the extract (individual polyphenols composition) were determined. The results showed that BEE modulates the microbial pattern and reduces inflammatory progression. The data demonstrated antioxidant properties correlated with the increase in synthesizing some biomarkers, such as SCFAs, which mitigated antibiotic-induced dysbiosis without using probiotic products.https://www.mdpi.com/2075-1729/13/7/1481patternporcinifunctional productin vitroshort-chain fatty acid synthesis |
| spellingShingle | Ionela Avram Diana Pelinescu Florentina Gatea Robertina Ionescu Alexandru Barcan Razvan Rosca Anca Zanfirescu Emanuel Vamanu <i>Boletus edulis</i> Extract—A New Modulator of Dysbiotic Microbiota Life pattern porcini functional product in vitro short-chain fatty acid synthesis |
| title | <i>Boletus edulis</i> Extract—A New Modulator of Dysbiotic Microbiota |
| title_full | <i>Boletus edulis</i> Extract—A New Modulator of Dysbiotic Microbiota |
| title_fullStr | <i>Boletus edulis</i> Extract—A New Modulator of Dysbiotic Microbiota |
| title_full_unstemmed | <i>Boletus edulis</i> Extract—A New Modulator of Dysbiotic Microbiota |
| title_short | <i>Boletus edulis</i> Extract—A New Modulator of Dysbiotic Microbiota |
| title_sort | i boletus edulis i extract a new modulator of dysbiotic microbiota |
| topic | pattern porcini functional product in vitro short-chain fatty acid synthesis |
| url | https://www.mdpi.com/2075-1729/13/7/1481 |
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