Kiwifruit (Actinidia chinensis ‘Hongyang’) cytosolic ascorbate peroxidases (AcAPX1 and AcAPX2) enhance salinity tolerance in Arabidopsis thaliana
Ascorbate peroxidase (APX) plays a key role in scavenging reactive oxygen species (ROS) in higher plants. However, there is very little information available on the APXs in kiwifruit (Actinidia), which is an economically and nutritionally important horticultural crop with exceptionally high ascorbic...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311921636523 |
| _version_ | 1818157382889373696 |
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| author | Xiu-hong GUO Yan HE Yu ZHANG Yi WANG Sheng-xiong HUANG Yong-sheng LIU Wei LI |
| author_facet | Xiu-hong GUO Yan HE Yu ZHANG Yi WANG Sheng-xiong HUANG Yong-sheng LIU Wei LI |
| author_sort | Xiu-hong GUO |
| collection | DOAJ |
| description | Ascorbate peroxidase (APX) plays a key role in scavenging reactive oxygen species (ROS) in higher plants. However, there is very little information available on the APXs in kiwifruit (Actinidia), which is an economically and nutritionally important horticultural crop with exceptionally high ascorbic acid (AsA) accumulation. This study aims to identify and characterize two cytosolic APX genes (AcAPX1 and AcAPX2) derived from A. chinensis ‘Hongyang’. The constitutive expression pattern was determined for both AcAPX1 and AcAPX2, and showed relatively higher expression abundances of AcAPX1 in leaf and AcAPX2 in root. Transcript levels of AcAPX1 and AcAPX2 were increased in kiwifruit roots treated with NaCl. Subcellular localization assays using GFP-fusion proteins in Arabidopsis protoplasts showed that both AcAPX1 and AcAPX2 are targeted to the cytosol. Recombinant AcAPX1 or AcAPX2 proteins were successfully expressed in the prokaryotic expression system and their individual ascorbate peroxidase activities were determined. Finally, constitutive over-expression of AcAPX1 or AcAPX2 could dramatically increase total AsA, glutathione level and salinity tolerance under NaCl stress in Arabidopsis thaliana. Our findings revealed that cytosolic AcAPX1/2 may play an important protective role in the responses to unfavorable environmental stimuli in kiwifruit. |
| first_indexed | 2024-12-11T15:13:19Z |
| format | Article |
| id | doaj.art-945f9bf4321245feb35e3fcdeeb44c86 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-11T15:13:19Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-945f9bf4321245feb35e3fcdeeb44c862022-12-22T01:00:41ZengElsevierJournal of Integrative Agriculture2095-31192022-04-0121410581070Kiwifruit (Actinidia chinensis ‘Hongyang’) cytosolic ascorbate peroxidases (AcAPX1 and AcAPX2) enhance salinity tolerance in Arabidopsis thalianaXiu-hong GUO0Yan HE1Yu ZHANG2Yi WANG3Sheng-xiong HUANG4Yong-sheng LIU5Wei LI6Key Laboratory for Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences/State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, P.R.ChinaSchool of Horticulture, Anhui Agricultural University, Hefei 230036, P.R.ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, P.R.ChinaSchool of Horticulture, Anhui Agricultural University, Hefei 230036, P.R.ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, P.R.ChinaKey Laboratory for Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences/State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, P.R.China; School of Horticulture, Anhui Agricultural University, Hefei 230036, P.R.China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, P.R.China; Correspondence LIU Yong-shengSchool of Horticulture, Anhui Agricultural University, Hefei 230036, P.R.China; Correspondence LI WeiAscorbate peroxidase (APX) plays a key role in scavenging reactive oxygen species (ROS) in higher plants. However, there is very little information available on the APXs in kiwifruit (Actinidia), which is an economically and nutritionally important horticultural crop with exceptionally high ascorbic acid (AsA) accumulation. This study aims to identify and characterize two cytosolic APX genes (AcAPX1 and AcAPX2) derived from A. chinensis ‘Hongyang’. The constitutive expression pattern was determined for both AcAPX1 and AcAPX2, and showed relatively higher expression abundances of AcAPX1 in leaf and AcAPX2 in root. Transcript levels of AcAPX1 and AcAPX2 were increased in kiwifruit roots treated with NaCl. Subcellular localization assays using GFP-fusion proteins in Arabidopsis protoplasts showed that both AcAPX1 and AcAPX2 are targeted to the cytosol. Recombinant AcAPX1 or AcAPX2 proteins were successfully expressed in the prokaryotic expression system and their individual ascorbate peroxidase activities were determined. Finally, constitutive over-expression of AcAPX1 or AcAPX2 could dramatically increase total AsA, glutathione level and salinity tolerance under NaCl stress in Arabidopsis thaliana. Our findings revealed that cytosolic AcAPX1/2 may play an important protective role in the responses to unfavorable environmental stimuli in kiwifruit.http://www.sciencedirect.com/science/article/pii/S2095311921636523Actinidiaascorbic acidascorbate peroxidasesalinity |
| spellingShingle | Xiu-hong GUO Yan HE Yu ZHANG Yi WANG Sheng-xiong HUANG Yong-sheng LIU Wei LI Kiwifruit (Actinidia chinensis ‘Hongyang’) cytosolic ascorbate peroxidases (AcAPX1 and AcAPX2) enhance salinity tolerance in Arabidopsis thaliana Journal of Integrative Agriculture Actinidia ascorbic acid ascorbate peroxidase salinity |
| title | Kiwifruit (Actinidia chinensis ‘Hongyang’) cytosolic ascorbate peroxidases (AcAPX1 and AcAPX2) enhance salinity tolerance in Arabidopsis thaliana |
| title_full | Kiwifruit (Actinidia chinensis ‘Hongyang’) cytosolic ascorbate peroxidases (AcAPX1 and AcAPX2) enhance salinity tolerance in Arabidopsis thaliana |
| title_fullStr | Kiwifruit (Actinidia chinensis ‘Hongyang’) cytosolic ascorbate peroxidases (AcAPX1 and AcAPX2) enhance salinity tolerance in Arabidopsis thaliana |
| title_full_unstemmed | Kiwifruit (Actinidia chinensis ‘Hongyang’) cytosolic ascorbate peroxidases (AcAPX1 and AcAPX2) enhance salinity tolerance in Arabidopsis thaliana |
| title_short | Kiwifruit (Actinidia chinensis ‘Hongyang’) cytosolic ascorbate peroxidases (AcAPX1 and AcAPX2) enhance salinity tolerance in Arabidopsis thaliana |
| title_sort | kiwifruit actinidia chinensis hongyang cytosolic ascorbate peroxidases acapx1 and acapx2 enhance salinity tolerance in arabidopsis thaliana |
| topic | Actinidia ascorbic acid ascorbate peroxidase salinity |
| url | http://www.sciencedirect.com/science/article/pii/S2095311921636523 |
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