Evaluation of Efflux-Mediated Resistance and Biofilm formation in Virulent <i>Pseudomonas aeruginosa</i> Associated with Healthcare Infections
<i>Pseudomonas aeruginosa</i> is a significant pathogen identified with healthcare-associated infections. The present study evaluates the role of biofilm and efflux pump activities in influencing high-level resistance in virulent <i>P. aeruginosa</i> strains in clinical infec...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/3/626 |
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| author | Paul A. Akinduti Onome W. George Hannah U. Ohore Olusegun E. Ariyo Samuel T. Popoola Adenike I. Adeleye Kazeem S. Akinwande Jacob O. Popoola Solomon O. Rotimi Fredrick O. Olufemi Conrad A. Omonhinmin Grace I. Olasehinde |
| author_facet | Paul A. Akinduti Onome W. George Hannah U. Ohore Olusegun E. Ariyo Samuel T. Popoola Adenike I. Adeleye Kazeem S. Akinwande Jacob O. Popoola Solomon O. Rotimi Fredrick O. Olufemi Conrad A. Omonhinmin Grace I. Olasehinde |
| author_sort | Paul A. Akinduti |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> is a significant pathogen identified with healthcare-associated infections. The present study evaluates the role of biofilm and efflux pump activities in influencing high-level resistance in virulent <i>P. aeruginosa</i> strains in clinical infection. Phenotypic resistance in biotyped <i>Pseudomonas aeruginosa</i> (<i>n</i> = 147) from diagnosed disease conditions was classified based on multiple antibiotic resistance (MAR) indices and analysed with logistic regression for risk factors. Efflux pump activity, biofilm formation, and virulence factors were analysed for optimal association in <i>Pseudomonas</i> infection using receiver operation characteristics (ROC). Age-specificity (OR [CI] = 0.986 [0.946–1.027]), gender (OR [CI] = 1.44 [0.211–9.827]) and infection sources (OR [CI] = 0.860 [0.438–1.688]) were risk variables for multidrug resistance (MDR)-<i>P. aeruginosa</i> infection (<i>p</i> < 0.05). Biofilm formers caused 48.2% and 18.5% otorrhea and wound infections (95% CI = 0.820–1.032; <i>p</i> = 0.001) respectively and more than 30% multidrug resistance (MDR) strains demonstrated high-level efflux pump activity (95% CI = 0.762–1.016; <i>p</i> = 0.001), protease (95% CI = 0.112–0.480; <i>p</i> = 0.003), lipase (95% CI = 0.143–0.523; <i>p</i> = 0.001), and hemolysin (95% CI = 1.109–1.780; <i>p</i> = 0.001). Resistance relatedness of more than 80% and 60% to cell wall biosynthesis inhibitors (ceftazidime, ceffproxil, augumentin, ampicillin) and, DNA translational and transcriptional inhibitors (gentamicin, ciprofloxacin, ofloxacin, nitrofurantoin) were observed (<i>p</i> < 0.05). Strong efflux correlation (r = 0.85, <i>p</i> = 0.034) with MDR strains, with high predictive performances in efflux pump activity (ROC-AUC 0.78), biofilm formation (ROC-AUC 0.520), and virulence hierarchical-clustering. Combine activities of the expressed efflux pump and biofilm formation in MDR-<i>P. aeruginosa</i> pose risk to clinical management and infection control. |
| first_indexed | 2024-03-11T07:00:44Z |
| format | Article |
| id | doaj.art-946202965d48448f9dd6a7b0defb570e |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T07:00:44Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-946202965d48448f9dd6a7b0defb570e2023-11-17T09:15:45ZengMDPI AGAntibiotics2079-63822023-03-0112362610.3390/antibiotics12030626Evaluation of Efflux-Mediated Resistance and Biofilm formation in Virulent <i>Pseudomonas aeruginosa</i> Associated with Healthcare InfectionsPaul A. Akinduti0Onome W. George1Hannah U. Ohore2Olusegun E. Ariyo3Samuel T. Popoola4Adenike I. Adeleye5Kazeem S. Akinwande6Jacob O. Popoola7Solomon O. Rotimi8Fredrick O. Olufemi9Conrad A. Omonhinmin10Grace I. Olasehinde11Microbiology Unit, Department of Biological Sciences, Covenant University, PMB 1023, Ota 112104, Ogun State, NigeriaMicrobiology Unit, Department of Biological Sciences, Covenant University, PMB 1023, Ota 112104, Ogun State, NigeriaMicrobiology Unit, Department of Biological Sciences, Covenant University, PMB 1023, Ota 112104, Ogun State, NigeriaObasanjo Holdings Limited, Abeokuta 110124, Ogun State, NigeriaMicrobiology Unit, Department of Biological Sciences, Covenant University, PMB 1023, Ota 112104, Ogun State, NigeriaVeterinary Teaching Hospital, Federal University of Agriculture, Abeokuta 110124, Ogun State, NigeriaDepartment of Chemical Pathology and Immunology, Federal Medical Centre, Abeokuta 110124, Ogun State, NigeriaApplied Biology and Biotechnology Unit, Department of Biological Sciences, Covenant University, PMB 1023, Ota 112104, Ogun State, NigeriaDepartment of Biochemistry and Molecular Biology, Covenant University, PMB 1023, Ota 112104, Ogun State, NigeriaDepartment of Veterinary Microbiology and Virology, College of Veterinary Medicine, Federal University of Agriculture, Abeokuta 110124, Ogun State, NigeriaApplied Biology and Biotechnology Unit, Department of Biological Sciences, Covenant University, PMB 1023, Ota 112104, Ogun State, NigeriaMicrobiology Unit, Department of Biological Sciences, Covenant University, PMB 1023, Ota 112104, Ogun State, Nigeria<i>Pseudomonas aeruginosa</i> is a significant pathogen identified with healthcare-associated infections. The present study evaluates the role of biofilm and efflux pump activities in influencing high-level resistance in virulent <i>P. aeruginosa</i> strains in clinical infection. Phenotypic resistance in biotyped <i>Pseudomonas aeruginosa</i> (<i>n</i> = 147) from diagnosed disease conditions was classified based on multiple antibiotic resistance (MAR) indices and analysed with logistic regression for risk factors. Efflux pump activity, biofilm formation, and virulence factors were analysed for optimal association in <i>Pseudomonas</i> infection using receiver operation characteristics (ROC). Age-specificity (OR [CI] = 0.986 [0.946–1.027]), gender (OR [CI] = 1.44 [0.211–9.827]) and infection sources (OR [CI] = 0.860 [0.438–1.688]) were risk variables for multidrug resistance (MDR)-<i>P. aeruginosa</i> infection (<i>p</i> < 0.05). Biofilm formers caused 48.2% and 18.5% otorrhea and wound infections (95% CI = 0.820–1.032; <i>p</i> = 0.001) respectively and more than 30% multidrug resistance (MDR) strains demonstrated high-level efflux pump activity (95% CI = 0.762–1.016; <i>p</i> = 0.001), protease (95% CI = 0.112–0.480; <i>p</i> = 0.003), lipase (95% CI = 0.143–0.523; <i>p</i> = 0.001), and hemolysin (95% CI = 1.109–1.780; <i>p</i> = 0.001). Resistance relatedness of more than 80% and 60% to cell wall biosynthesis inhibitors (ceftazidime, ceffproxil, augumentin, ampicillin) and, DNA translational and transcriptional inhibitors (gentamicin, ciprofloxacin, ofloxacin, nitrofurantoin) were observed (<i>p</i> < 0.05). Strong efflux correlation (r = 0.85, <i>p</i> = 0.034) with MDR strains, with high predictive performances in efflux pump activity (ROC-AUC 0.78), biofilm formation (ROC-AUC 0.520), and virulence hierarchical-clustering. Combine activities of the expressed efflux pump and biofilm formation in MDR-<i>P. aeruginosa</i> pose risk to clinical management and infection control.https://www.mdpi.com/2079-6382/12/3/626<i>Pseudomonas aeruginosa</i>antibiotic resistancebiofilmefflux pumpvirulence factors |
| spellingShingle | Paul A. Akinduti Onome W. George Hannah U. Ohore Olusegun E. Ariyo Samuel T. Popoola Adenike I. Adeleye Kazeem S. Akinwande Jacob O. Popoola Solomon O. Rotimi Fredrick O. Olufemi Conrad A. Omonhinmin Grace I. Olasehinde Evaluation of Efflux-Mediated Resistance and Biofilm formation in Virulent <i>Pseudomonas aeruginosa</i> Associated with Healthcare Infections Antibiotics <i>Pseudomonas aeruginosa</i> antibiotic resistance biofilm efflux pump virulence factors |
| title | Evaluation of Efflux-Mediated Resistance and Biofilm formation in Virulent <i>Pseudomonas aeruginosa</i> Associated with Healthcare Infections |
| title_full | Evaluation of Efflux-Mediated Resistance and Biofilm formation in Virulent <i>Pseudomonas aeruginosa</i> Associated with Healthcare Infections |
| title_fullStr | Evaluation of Efflux-Mediated Resistance and Biofilm formation in Virulent <i>Pseudomonas aeruginosa</i> Associated with Healthcare Infections |
| title_full_unstemmed | Evaluation of Efflux-Mediated Resistance and Biofilm formation in Virulent <i>Pseudomonas aeruginosa</i> Associated with Healthcare Infections |
| title_short | Evaluation of Efflux-Mediated Resistance and Biofilm formation in Virulent <i>Pseudomonas aeruginosa</i> Associated with Healthcare Infections |
| title_sort | evaluation of efflux mediated resistance and biofilm formation in virulent i pseudomonas aeruginosa i associated with healthcare infections |
| topic | <i>Pseudomonas aeruginosa</i> antibiotic resistance biofilm efflux pump virulence factors |
| url | https://www.mdpi.com/2079-6382/12/3/626 |
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