Molecular and serological detection of Neospora caninum in multiple tissues and CSF in asymptomatic infected stray dogs.
Backgrounds: Neospora caninum is an Apicomplexan parasite. It causes paralysis and neuromuscular disorders in dogs and abortion in cattle. Although contamination with N. caninum is common in stray dogs, most of the dogs are infected with subclinical neosporosis. Objectives: The objective of this stu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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University of Tehran
2017-04-01
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| Series: | Iranian Journal of Veterinary Medicine |
| Subjects: | |
| Online Access: | https://ijvm.ut.ac.ir/article_61602_ed973cd720d2fe792c85b1638cfc256f.pdf |
| _version_ | 1819122114747170816 |
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| author | Ali Pouramini Shahram Jamshidi Parviz Shayan Elahe Ebrahimzadeh Mehdi Namavari Sadegh Shirian |
| author_facet | Ali Pouramini Shahram Jamshidi Parviz Shayan Elahe Ebrahimzadeh Mehdi Namavari Sadegh Shirian |
| author_sort | Ali Pouramini |
| collection | DOAJ |
| description | Backgrounds: Neospora caninum is an Apicomplexan parasite. It causes paralysis and neuromuscular disorders in dogs and abortion in cattle. Although contamination with N. caninum is common in stray dogs, most of the dogs are infected with subclinical neosporosis. Objectives: The objective of this study was to evaluate the presence of N. caninum in multiple tissues and cerebrospinal fluid (CSF) using Nested-PCR technique. Furthermore, the N. caninum specific antibody was detected in serum of examined dogs by indirect enzyme-linked immunosorbent assay (ELISA). Methods: Forty two stray dogs of mixed breeds captured from districts of Tehran, Iran, were examined physically and euthanized. A commercially indirect ELISA kit was used to detect the anti-N. caninum antibodies in serum. Nested PCR was applied to analyze the extracted DNA from brain, skeletal muscle, CSF, liver, spleen and mandibular lymph nodes for Nc5 gene. Results: Indirect ELISA assay for N. caninum antibody was positive in a seven years old male dog (2.22%). Out of 42 stray dogs whose multiple organs were examined using Nested PCR, 15 samples (35%) were positive. The highest presence of N. caninum was found in skeletal muscle with 30% (13/42) frequency, followed by CSF (26.2%) (11/42), brain (19%) (8/42), liver (7.14%) (3/42), lymph node (4.62%) (2/42) and spleen samples (0/42). Conclusions: These results suggest that serology tests can underestimate the carrier of N. caninum in dogs compared to PCR test which directly detect the presence of the parasite. Nested-PCR might be a potential alternative for the histological methods to detect N. caninum in dog’s tissues. |
| first_indexed | 2024-12-22T06:47:19Z |
| format | Article |
| id | doaj.art-946313b441e2473c9a4bb3c585518888 |
| institution | Directory Open Access Journal |
| issn | 2251-8894 2252-0554 |
| language | English |
| last_indexed | 2024-12-22T06:47:19Z |
| publishDate | 2017-04-01 |
| publisher | University of Tehran |
| record_format | Article |
| series | Iranian Journal of Veterinary Medicine |
| spelling | doaj.art-946313b441e2473c9a4bb3c5855188882022-12-21T18:35:15ZengUniversity of TehranIranian Journal of Veterinary Medicine2251-88942252-05542017-04-0111210511210.22059/ijvm.2017.6160261602Molecular and serological detection of Neospora caninum in multiple tissues and CSF in asymptomatic infected stray dogs.Ali Pouramini0Shahram Jamshidi1Parviz Shayan2Elahe Ebrahimzadeh3Mehdi Namavari4Sadegh Shirian5Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.Razi Vaccine and Serum Research Institute, Shiraz, Iran.Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrkord, Iran.Backgrounds: Neospora caninum is an Apicomplexan parasite. It causes paralysis and neuromuscular disorders in dogs and abortion in cattle. Although contamination with N. caninum is common in stray dogs, most of the dogs are infected with subclinical neosporosis. Objectives: The objective of this study was to evaluate the presence of N. caninum in multiple tissues and cerebrospinal fluid (CSF) using Nested-PCR technique. Furthermore, the N. caninum specific antibody was detected in serum of examined dogs by indirect enzyme-linked immunosorbent assay (ELISA). Methods: Forty two stray dogs of mixed breeds captured from districts of Tehran, Iran, were examined physically and euthanized. A commercially indirect ELISA kit was used to detect the anti-N. caninum antibodies in serum. Nested PCR was applied to analyze the extracted DNA from brain, skeletal muscle, CSF, liver, spleen and mandibular lymph nodes for Nc5 gene. Results: Indirect ELISA assay for N. caninum antibody was positive in a seven years old male dog (2.22%). Out of 42 stray dogs whose multiple organs were examined using Nested PCR, 15 samples (35%) were positive. The highest presence of N. caninum was found in skeletal muscle with 30% (13/42) frequency, followed by CSF (26.2%) (11/42), brain (19%) (8/42), liver (7.14%) (3/42), lymph node (4.62%) (2/42) and spleen samples (0/42). Conclusions: These results suggest that serology tests can underestimate the carrier of N. caninum in dogs compared to PCR test which directly detect the presence of the parasite. Nested-PCR might be a potential alternative for the histological methods to detect N. caninum in dog’s tissues.https://ijvm.ut.ac.ir/article_61602_ed973cd720d2fe792c85b1638cfc256f.pdfdogindirect elisaneospora caninumneosporosisnested-pcr |
| spellingShingle | Ali Pouramini Shahram Jamshidi Parviz Shayan Elahe Ebrahimzadeh Mehdi Namavari Sadegh Shirian Molecular and serological detection of Neospora caninum in multiple tissues and CSF in asymptomatic infected stray dogs. Iranian Journal of Veterinary Medicine dog indirect elisa neospora caninum neosporosis nested-pcr |
| title | Molecular and serological detection of Neospora caninum in multiple tissues and CSF in asymptomatic infected stray dogs. |
| title_full | Molecular and serological detection of Neospora caninum in multiple tissues and CSF in asymptomatic infected stray dogs. |
| title_fullStr | Molecular and serological detection of Neospora caninum in multiple tissues and CSF in asymptomatic infected stray dogs. |
| title_full_unstemmed | Molecular and serological detection of Neospora caninum in multiple tissues and CSF in asymptomatic infected stray dogs. |
| title_short | Molecular and serological detection of Neospora caninum in multiple tissues and CSF in asymptomatic infected stray dogs. |
| title_sort | molecular and serological detection of neospora caninum in multiple tissues and csf in asymptomatic infected stray dogs |
| topic | dog indirect elisa neospora caninum neosporosis nested-pcr |
| url | https://ijvm.ut.ac.ir/article_61602_ed973cd720d2fe792c85b1638cfc256f.pdf |
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