Genome-Wide Analysis and Expression Profiling of the Phospholipase D Gene Family in <i>Solanum tuberosum</i>
Phospholipase D (PLD) is the most important phospholipid hydrolase in plants, which can hydrolyze phospholipids into phosphatidic acid (PA) and choline. When plants encounter low temperature, drought and high salt stress, phospholipase D and its products play an important role in regulating plant gr...
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MDPI AG
2021-08-01
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| author | Long Li Chao Zhang Mancang Zhang Chenghui Yang Yanru Bao Dongdong Wang Qin Chen Yue Chen |
| author_facet | Long Li Chao Zhang Mancang Zhang Chenghui Yang Yanru Bao Dongdong Wang Qin Chen Yue Chen |
| author_sort | Long Li |
| collection | DOAJ |
| description | Phospholipase D (PLD) is the most important phospholipid hydrolase in plants, which can hydrolyze phospholipids into phosphatidic acid (PA) and choline. When plants encounter low temperature, drought and high salt stress, phospholipase D and its products play an important role in regulating plant growth and development and coping with stress. In this study, 16 members of StPLD gene family were identified in potato genome, which were distributed in α, β, δ, and ζ subfamilies, and their expression patterns under salt, high temperature, drought, and ABA stress were detected by qRT-PCR method. Gene expression analysis showed that the expression of StPLD genes in potato was upregulated and downregulated to varying degrees under the four stresses, indicating that the PLD gene family is involved in the interaction of potato plant hormones and abiotic stress signals. Chromosome distribution showed that StPLD gene was unevenly distributed on 8 chromosomes, and only one pair of tandem repeat genes was found. All StPLD promoters contain hormone and stress-related cis-regulatory elements to respond to different stresses. Structural analysis showed that StPLD genes in the same subgroup had a similar exon–intron structure. Our study provides a valuable reference for further research of the function and structure of PLD gene. |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T09:00:33Z |
| publishDate | 2021-08-01 |
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| spelling | doaj.art-946577576b034342b417f6f6dba10e252023-11-22T06:49:51ZengMDPI AGBiology2079-77372021-08-0110874110.3390/biology10080741Genome-Wide Analysis and Expression Profiling of the Phospholipase D Gene Family in <i>Solanum tuberosum</i>Long Li0Chao Zhang1Mancang Zhang2Chenghui Yang3Yanru Bao4Dongdong Wang5Qin Chen6Yue Chen7State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaPhospholipase D (PLD) is the most important phospholipid hydrolase in plants, which can hydrolyze phospholipids into phosphatidic acid (PA) and choline. When plants encounter low temperature, drought and high salt stress, phospholipase D and its products play an important role in regulating plant growth and development and coping with stress. In this study, 16 members of StPLD gene family were identified in potato genome, which were distributed in α, β, δ, and ζ subfamilies, and their expression patterns under salt, high temperature, drought, and ABA stress were detected by qRT-PCR method. Gene expression analysis showed that the expression of StPLD genes in potato was upregulated and downregulated to varying degrees under the four stresses, indicating that the PLD gene family is involved in the interaction of potato plant hormones and abiotic stress signals. Chromosome distribution showed that StPLD gene was unevenly distributed on 8 chromosomes, and only one pair of tandem repeat genes was found. All StPLD promoters contain hormone and stress-related cis-regulatory elements to respond to different stresses. Structural analysis showed that StPLD genes in the same subgroup had a similar exon–intron structure. Our study provides a valuable reference for further research of the function and structure of PLD gene.https://www.mdpi.com/2079-7737/10/8/741potatoPLDabiotic stressgene expression |
| spellingShingle | Long Li Chao Zhang Mancang Zhang Chenghui Yang Yanru Bao Dongdong Wang Qin Chen Yue Chen Genome-Wide Analysis and Expression Profiling of the Phospholipase D Gene Family in <i>Solanum tuberosum</i> Biology potato PLD abiotic stress gene expression |
| title | Genome-Wide Analysis and Expression Profiling of the Phospholipase D Gene Family in <i>Solanum tuberosum</i> |
| title_full | Genome-Wide Analysis and Expression Profiling of the Phospholipase D Gene Family in <i>Solanum tuberosum</i> |
| title_fullStr | Genome-Wide Analysis and Expression Profiling of the Phospholipase D Gene Family in <i>Solanum tuberosum</i> |
| title_full_unstemmed | Genome-Wide Analysis and Expression Profiling of the Phospholipase D Gene Family in <i>Solanum tuberosum</i> |
| title_short | Genome-Wide Analysis and Expression Profiling of the Phospholipase D Gene Family in <i>Solanum tuberosum</i> |
| title_sort | genome wide analysis and expression profiling of the phospholipase d gene family in i solanum tuberosum i |
| topic | potato PLD abiotic stress gene expression |
| url | https://www.mdpi.com/2079-7737/10/8/741 |
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