Isolation and Characterization of a Lytic Vibriophage OY1 and Its Biocontrol Effects Against Vibrio spp.
Vibrio species are important pathogens of marine animals and aquaculture populations and some of them can cause serious infections in humans through consumption of contaminated seafood and aquaculture products. Lytic bacteriophages can potentially alleviate Vibrio contamination in the aquaculture or...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.830692/full |
| _version_ | 1811313241411813376 |
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| author | Lu Gao Lu Gao Min Ouyang Yi Li Hui Zhang Xiang-Feng Zheng Hua-Xiang Li Sheng-Qi Rao Zhen-Quan Yang Zhen-Quan Yang Song Gao Song Gao |
| author_facet | Lu Gao Lu Gao Min Ouyang Yi Li Hui Zhang Xiang-Feng Zheng Hua-Xiang Li Sheng-Qi Rao Zhen-Quan Yang Zhen-Quan Yang Song Gao Song Gao |
| author_sort | Lu Gao |
| collection | DOAJ |
| description | Vibrio species are important pathogens of marine animals and aquaculture populations and some of them can cause serious infections in humans through consumption of contaminated seafood and aquaculture products. Lytic bacteriophages can potentially alleviate Vibrio contamination in the aquaculture organisms and in the processing of aquatic products and have gained significant scientific attention in recent years. In the present study, bacteriophages were isolated from sewage of local aquatic products markets and grown using Vibrio mimicus CICC 21613 as host cells. The lytic vibriophage OY1 belonging to the newly proposed family Autographiviridae and the genus Maculvirus was identified by observation under electron microscope and comparative genomic analysis. The phage OY1 showed lytic activity against 24 among 32 tested strains belonging to eight Vibrio species. The complete phage OY1 genome consists of a single circular double-stranded DNA of 43,479 bp with a total GC content of 49.27% and was predicted to encode 40 open reading frames (ORFs). To evaluate its potential against vibrios, the one-step growth curve, thermal and pH stability, host range, and lytic activity of the OY1 phage against Vibrio species were evaluated. The results showed that phage OY1 had a range of thermal and pH tolerance, and exhibited a significant inhibitory effect on the growth of tested Vibrio species. Bacterial growth in the fish muscle extract juice (FMEJ) inoculated with Vibrio mimicus CICC 21613, Vibrio parahaemolyticus CICC 21617, Vibrio alginolyticus VJ14, and the mixed bacterial culture was reduced by 2.65 log CFU/ml, 2.42 log CFU/ml, 1.93 log CFU/ml, and 2.01 log CFU/ml, respectively, by incubation with phage OY1 at 25°C for 36 h. Phage OY1 also showed a strong ability to prevent biofilm formation and destroy formed Vibrio species biofilms. These results indicate that phage OY1 is a potential biocontrol agent against Vibrio species in the aquaculture industry and in food safety control. |
| first_indexed | 2024-04-13T10:50:17Z |
| format | Article |
| id | doaj.art-9469ccf93c00431c8793aa00f90b7ad5 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T10:50:17Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9469ccf93c00431c8793aa00f90b7ad52022-12-22T02:49:41ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-04-011310.3389/fmicb.2022.830692830692Isolation and Characterization of a Lytic Vibriophage OY1 and Its Biocontrol Effects Against Vibrio spp.Lu Gao0Lu Gao1Min Ouyang2Yi Li3Hui Zhang4Xiang-Feng Zheng5Hua-Xiang Li6Sheng-Qi Rao7Zhen-Quan Yang8Zhen-Quan Yang9Song Gao10Song Gao11College of Food Science and Engineering, Yangzhou University, Yangzhou, ChinaKey Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agri-food Safety and Quality, Ministry of Agriculture, Yangzhou, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, ChinaKey Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agri-food Safety and Quality, Ministry of Agriculture, Yangzhou, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaVibrio species are important pathogens of marine animals and aquaculture populations and some of them can cause serious infections in humans through consumption of contaminated seafood and aquaculture products. Lytic bacteriophages can potentially alleviate Vibrio contamination in the aquaculture organisms and in the processing of aquatic products and have gained significant scientific attention in recent years. In the present study, bacteriophages were isolated from sewage of local aquatic products markets and grown using Vibrio mimicus CICC 21613 as host cells. The lytic vibriophage OY1 belonging to the newly proposed family Autographiviridae and the genus Maculvirus was identified by observation under electron microscope and comparative genomic analysis. The phage OY1 showed lytic activity against 24 among 32 tested strains belonging to eight Vibrio species. The complete phage OY1 genome consists of a single circular double-stranded DNA of 43,479 bp with a total GC content of 49.27% and was predicted to encode 40 open reading frames (ORFs). To evaluate its potential against vibrios, the one-step growth curve, thermal and pH stability, host range, and lytic activity of the OY1 phage against Vibrio species were evaluated. The results showed that phage OY1 had a range of thermal and pH tolerance, and exhibited a significant inhibitory effect on the growth of tested Vibrio species. Bacterial growth in the fish muscle extract juice (FMEJ) inoculated with Vibrio mimicus CICC 21613, Vibrio parahaemolyticus CICC 21617, Vibrio alginolyticus VJ14, and the mixed bacterial culture was reduced by 2.65 log CFU/ml, 2.42 log CFU/ml, 1.93 log CFU/ml, and 2.01 log CFU/ml, respectively, by incubation with phage OY1 at 25°C for 36 h. Phage OY1 also showed a strong ability to prevent biofilm formation and destroy formed Vibrio species biofilms. These results indicate that phage OY1 is a potential biocontrol agent against Vibrio species in the aquaculture industry and in food safety control.https://www.frontiersin.org/articles/10.3389/fmicb.2022.830692/fullvibriophagelytic activitybiocontrolVibrio speciesbiofilm |
| spellingShingle | Lu Gao Lu Gao Min Ouyang Yi Li Hui Zhang Xiang-Feng Zheng Hua-Xiang Li Sheng-Qi Rao Zhen-Quan Yang Zhen-Quan Yang Song Gao Song Gao Isolation and Characterization of a Lytic Vibriophage OY1 and Its Biocontrol Effects Against Vibrio spp. Frontiers in Microbiology vibriophage lytic activity biocontrol Vibrio species biofilm |
| title | Isolation and Characterization of a Lytic Vibriophage OY1 and Its Biocontrol Effects Against Vibrio spp. |
| title_full | Isolation and Characterization of a Lytic Vibriophage OY1 and Its Biocontrol Effects Against Vibrio spp. |
| title_fullStr | Isolation and Characterization of a Lytic Vibriophage OY1 and Its Biocontrol Effects Against Vibrio spp. |
| title_full_unstemmed | Isolation and Characterization of a Lytic Vibriophage OY1 and Its Biocontrol Effects Against Vibrio spp. |
| title_short | Isolation and Characterization of a Lytic Vibriophage OY1 and Its Biocontrol Effects Against Vibrio spp. |
| title_sort | isolation and characterization of a lytic vibriophage oy1 and its biocontrol effects against vibrio spp |
| topic | vibriophage lytic activity biocontrol Vibrio species biofilm |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.830692/full |
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