Effects of culture medium and lighting condition on induction of conidiation in Cercospora citrullina
Cercospora citrullina, isolated from pumpkin plant (Cucurbita maxima), initially could not produce conidia when cultured on potato dextrose agar, but was able to produce conidia when cultured on oatmeal agar, oat grain agar, wheat grain agar and barley grain agar. C. citrullina also produced conid...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Prince of Songkla University
2020-08-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | https://rdo.psu.ac.th/sjstweb/journal/42-4/29.pdf |
| _version_ | 1818334208613941248 |
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| author | Kathrine Xin Yee Tan Azlinda Binti Ibrahim Hideyuki Nagao |
| author_facet | Kathrine Xin Yee Tan Azlinda Binti Ibrahim Hideyuki Nagao |
| author_sort | Kathrine Xin Yee Tan |
| collection | DOAJ |
| description | Cercospora citrullina, isolated from pumpkin plant (Cucurbita maxima), initially could not produce conidia when
cultured on potato dextrose agar, but was able to produce conidia when cultured on oatmeal agar, oat grain agar, wheat grain agar
and barley grain agar. C. citrullina also produced conidia when cultured in substrates such as oat grain, wheat grain, barley grain
and Trichosanthes sp. dried leaves. Trichosanthes sp. dried leaves induced the highest conidia production (4.81 × 103
conidia /
ml) while oatmeal agar and barley grain agar yielded the best result among the agar media by inducing 1.23 × 103
conidia / ml
each. Overall, continuous darkness condition was better than other lighting conditions (12 h near UV black light plus 12 h
darkness condition; and 12 h fluorescent white light plus 12 h darkness lighting condition). Lastly, inoculation of C. citrullina
onto cucurbit plants was successfully conducted by sandwiching inoculum between leaves and plastic cards. |
| first_indexed | 2024-12-13T14:03:53Z |
| format | Article |
| id | doaj.art-9469ef230bad4f0d9e289387761fb0e2 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-13T14:03:53Z |
| publishDate | 2020-08-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-9469ef230bad4f0d9e289387761fb0e22022-12-21T23:42:39ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952020-08-0142493594010.14456/sjst-psu.2020.120Effects of culture medium and lighting condition on induction of conidiation in Cercospora citrullinaKathrine Xin Yee Tan0Azlinda Binti Ibrahim1Hideyuki Nagao2School of Biological Sciences, Universiti Sains Malaysia, Minden, Penang, 11800 MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, Minden, Penang, 11800 MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, Minden, Penang, 11800 MalaysiaCercospora citrullina, isolated from pumpkin plant (Cucurbita maxima), initially could not produce conidia when cultured on potato dextrose agar, but was able to produce conidia when cultured on oatmeal agar, oat grain agar, wheat grain agar and barley grain agar. C. citrullina also produced conidia when cultured in substrates such as oat grain, wheat grain, barley grain and Trichosanthes sp. dried leaves. Trichosanthes sp. dried leaves induced the highest conidia production (4.81 × 103 conidia / ml) while oatmeal agar and barley grain agar yielded the best result among the agar media by inducing 1.23 × 103 conidia / ml each. Overall, continuous darkness condition was better than other lighting conditions (12 h near UV black light plus 12 h darkness condition; and 12 h fluorescent white light plus 12 h darkness lighting condition). Lastly, inoculation of C. citrullina onto cucurbit plants was successfully conducted by sandwiching inoculum between leaves and plastic cards.https://rdo.psu.ac.th/sjstweb/journal/42-4/29.pdfcercosporaconidiamediumlighting conditionsinoculation |
| spellingShingle | Kathrine Xin Yee Tan Azlinda Binti Ibrahim Hideyuki Nagao Effects of culture medium and lighting condition on induction of conidiation in Cercospora citrullina Songklanakarin Journal of Science and Technology (SJST) cercospora conidia medium lighting conditions inoculation |
| title | Effects of culture medium and lighting condition on induction of conidiation in Cercospora citrullina |
| title_full | Effects of culture medium and lighting condition on induction of conidiation in Cercospora citrullina |
| title_fullStr | Effects of culture medium and lighting condition on induction of conidiation in Cercospora citrullina |
| title_full_unstemmed | Effects of culture medium and lighting condition on induction of conidiation in Cercospora citrullina |
| title_short | Effects of culture medium and lighting condition on induction of conidiation in Cercospora citrullina |
| title_sort | effects of culture medium and lighting condition on induction of conidiation in cercospora citrullina |
| topic | cercospora conidia medium lighting conditions inoculation |
| url | https://rdo.psu.ac.th/sjstweb/journal/42-4/29.pdf |
| work_keys_str_mv | AT kathrinexinyeetan effectsofculturemediumandlightingconditiononinductionofconidiationincercosporacitrullina AT azlindabintiibrahim effectsofculturemediumandlightingconditiononinductionofconidiationincercosporacitrullina AT hideyukinagao effectsofculturemediumandlightingconditiononinductionofconidiationincercosporacitrullina |