Multi-domain automated patterning of DNA-functionalized hydrogels.
DNA-functionalized hydrogels are capable of sensing oligonucleotides, proteins, and small molecules, and specific DNA sequences sensed in the hydrogels' environment can induce changes in these hydrogels' shape and fluorescence. Fabricating DNA-functionalized hydrogel architectures with mul...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2024-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0295923&type=printable |
| _version_ | 1797315927228809216 |
|---|---|
| author | Moshe Rubanov Joshua Cole Heon-Joon Lee Leandro G Soto Cordova Zachary Chen Elia Gonzalez Rebecca Schulman |
| author_facet | Moshe Rubanov Joshua Cole Heon-Joon Lee Leandro G Soto Cordova Zachary Chen Elia Gonzalez Rebecca Schulman |
| author_sort | Moshe Rubanov |
| collection | DOAJ |
| description | DNA-functionalized hydrogels are capable of sensing oligonucleotides, proteins, and small molecules, and specific DNA sequences sensed in the hydrogels' environment can induce changes in these hydrogels' shape and fluorescence. Fabricating DNA-functionalized hydrogel architectures with multiple domains could make it possible to sense multiple molecules and undergo more complicated macroscopic changes, such as changing fluorescence or changing the shapes of regions of the hydrogel architecture. However, automatically fabricating multi-domain DNA-functionalized hydrogel architectures, capable of enabling the construction of hydrogel architectures with tens to hundreds of different domains, presents a significant challenge. We describe a platform for fabricating multi-domain DNA-functionalized hydrogels automatically at the micron scale, where reaction and diffusion processes can be coupled to program material behavior. Using this platform, the hydrogels' material properties, such as shape and fluorescence, can be programmed, and the fabricated hydrogels can sense their environment. DNA-functionalized hydrogel architectures with domain sizes as small as 10 microns and with up to 4 different types of domains can be automatically fabricated using ink volumes as low as 50 μL. We also demonstrate that hydrogels fabricated using this platform exhibit responses similar to those of DNA-functionalized hydrogels fabricated using other methods by demonstrating that DNA sequences can hybridize within them and that they can undergo DNA sequence-induced shape change. |
| first_indexed | 2024-03-08T03:10:57Z |
| format | Article |
| id | doaj.art-946bced686a54fbd802c354a4875ff8c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-08T03:10:57Z |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-946bced686a54fbd802c354a4875ff8c2024-02-13T05:33:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01192e029592310.1371/journal.pone.0295923Multi-domain automated patterning of DNA-functionalized hydrogels.Moshe RubanovJoshua ColeHeon-Joon LeeLeandro G Soto CordovaZachary ChenElia GonzalezRebecca SchulmanDNA-functionalized hydrogels are capable of sensing oligonucleotides, proteins, and small molecules, and specific DNA sequences sensed in the hydrogels' environment can induce changes in these hydrogels' shape and fluorescence. Fabricating DNA-functionalized hydrogel architectures with multiple domains could make it possible to sense multiple molecules and undergo more complicated macroscopic changes, such as changing fluorescence or changing the shapes of regions of the hydrogel architecture. However, automatically fabricating multi-domain DNA-functionalized hydrogel architectures, capable of enabling the construction of hydrogel architectures with tens to hundreds of different domains, presents a significant challenge. We describe a platform for fabricating multi-domain DNA-functionalized hydrogels automatically at the micron scale, where reaction and diffusion processes can be coupled to program material behavior. Using this platform, the hydrogels' material properties, such as shape and fluorescence, can be programmed, and the fabricated hydrogels can sense their environment. DNA-functionalized hydrogel architectures with domain sizes as small as 10 microns and with up to 4 different types of domains can be automatically fabricated using ink volumes as low as 50 μL. We also demonstrate that hydrogels fabricated using this platform exhibit responses similar to those of DNA-functionalized hydrogels fabricated using other methods by demonstrating that DNA sequences can hybridize within them and that they can undergo DNA sequence-induced shape change.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0295923&type=printable |
| spellingShingle | Moshe Rubanov Joshua Cole Heon-Joon Lee Leandro G Soto Cordova Zachary Chen Elia Gonzalez Rebecca Schulman Multi-domain automated patterning of DNA-functionalized hydrogels. PLoS ONE |
| title | Multi-domain automated patterning of DNA-functionalized hydrogels. |
| title_full | Multi-domain automated patterning of DNA-functionalized hydrogels. |
| title_fullStr | Multi-domain automated patterning of DNA-functionalized hydrogels. |
| title_full_unstemmed | Multi-domain automated patterning of DNA-functionalized hydrogels. |
| title_short | Multi-domain automated patterning of DNA-functionalized hydrogels. |
| title_sort | multi domain automated patterning of dna functionalized hydrogels |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0295923&type=printable |
| work_keys_str_mv | AT mosherubanov multidomainautomatedpatterningofdnafunctionalizedhydrogels AT joshuacole multidomainautomatedpatterningofdnafunctionalizedhydrogels AT heonjoonlee multidomainautomatedpatterningofdnafunctionalizedhydrogels AT leandrogsotocordova multidomainautomatedpatterningofdnafunctionalizedhydrogels AT zacharychen multidomainautomatedpatterningofdnafunctionalizedhydrogels AT eliagonzalez multidomainautomatedpatterningofdnafunctionalizedhydrogels AT rebeccaschulman multidomainautomatedpatterningofdnafunctionalizedhydrogels |