Transcriptional Memory-Like Imprints and Enhanced Functional Activity in γδ T Cells Following Resolution of Malaria Infection
γδ T cells play an essential role in the immune response to many pathogens, including Plasmodium. However, long-lasting effects of infection on the γδ T cell population still remain inadequately understood. This study focused on assessing molecular and functional changes that persist in the γδ T cel...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2020.582358/full |
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| author | Rasika Kumarasingha Rasika Kumarasingha Lisa J. Ioannidis Lisa J. Ioannidis Waruni Abeysekera Waruni Abeysekera Stephanie Studniberg Stephanie Studniberg Dinidu Wijesurendra Dinidu Wijesurendra Ramin Mazhari Ramin Mazhari Daniel P. Poole Ivo Mueller Ivo Mueller Louis Schofield Louis Schofield Louis Schofield Diana S. Hansen Diana S. Hansen Emily M. Eriksson Emily M. Eriksson |
| author_facet | Rasika Kumarasingha Rasika Kumarasingha Lisa J. Ioannidis Lisa J. Ioannidis Waruni Abeysekera Waruni Abeysekera Stephanie Studniberg Stephanie Studniberg Dinidu Wijesurendra Dinidu Wijesurendra Ramin Mazhari Ramin Mazhari Daniel P. Poole Ivo Mueller Ivo Mueller Louis Schofield Louis Schofield Louis Schofield Diana S. Hansen Diana S. Hansen Emily M. Eriksson Emily M. Eriksson |
| author_sort | Rasika Kumarasingha |
| collection | DOAJ |
| description | γδ T cells play an essential role in the immune response to many pathogens, including Plasmodium. However, long-lasting effects of infection on the γδ T cell population still remain inadequately understood. This study focused on assessing molecular and functional changes that persist in the γδ T cell population following resolution of malaria infection. We investigated transcriptional changes and memory-like functional capacity of malaria pre-exposed γδ T cells using a Plasmodiumchabaudi infection model. We show that multiple genes associated with effector function (chemokines, cytokines and cytotoxicity) and antigen-presentation were upregulated in P. chabaudi-exposed γδ T cells compared to γδ T cells from naïve mice. This transcriptional profile was positively correlated with profiles observed in conventional memory CD8+ T cells and was accompanied by enhanced reactivation upon secondary encounter with Plasmodium-infected red blood cells in vitro. Collectively our data demonstrate that Plasmodium exposure result in “memory-like imprints” in the γδ T cell population and also promotes γδ T cells that can support antigen-presentation during subsequent infections. |
| first_indexed | 2024-12-12T05:50:48Z |
| format | Article |
| id | doaj.art-946c703f38414c428db6dd6e4799c84d |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-12T05:50:48Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-946c703f38414c428db6dd6e4799c84d2022-12-22T00:35:40ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-10-011110.3389/fimmu.2020.582358582358Transcriptional Memory-Like Imprints and Enhanced Functional Activity in γδ T Cells Following Resolution of Malaria InfectionRasika Kumarasingha0Rasika Kumarasingha1Lisa J. Ioannidis2Lisa J. Ioannidis3Waruni Abeysekera4Waruni Abeysekera5Stephanie Studniberg6Stephanie Studniberg7Dinidu Wijesurendra8Dinidu Wijesurendra9Ramin Mazhari10Ramin Mazhari11Daniel P. Poole12Ivo Mueller13Ivo Mueller14Louis Schofield15Louis Schofield16Louis Schofield17Diana S. Hansen18Diana S. Hansen19Emily M. Eriksson20Emily M. Eriksson21Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, AustraliaDepartment of Medical Biology, The University of Melbourne, Parkville, VIC, AustraliaWalter and Eliza Hall Institute of Medical Research, Parkville, VIC, AustraliaDepartment of Medical Biology, The University of Melbourne, Parkville, VIC, AustraliaWalter and Eliza Hall Institute of Medical Research, Parkville, VIC, AustraliaDepartment of Medical Biology, The University of Melbourne, Parkville, VIC, AustraliaWalter and Eliza Hall Institute of Medical Research, Parkville, VIC, AustraliaDepartment of Medical Biology, The University of Melbourne, Parkville, VIC, AustraliaWalter and Eliza Hall Institute of Medical Research, Parkville, VIC, AustraliaDepartment of Medical Biology, The University of Melbourne, Parkville, VIC, AustraliaWalter and Eliza Hall Institute of Medical Research, Parkville, VIC, AustraliaDepartment of Medical Biology, The University of Melbourne, Parkville, VIC, AustraliaDrug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, AustraliaWalter and Eliza Hall Institute of Medical Research, Parkville, VIC, AustraliaDepartment of Medical Biology, The University of Melbourne, Parkville, VIC, AustraliaWalter and Eliza Hall Institute of Medical Research, Parkville, VIC, AustraliaSchool of Veterinary and Biomedical Sciences, James Cook University, Townsville, QLD, AustraliaAustralian Institute of Tropical Health and Medicine, James Cook University, Smithfield, QLD, AustraliaWalter and Eliza Hall Institute of Medical Research, Parkville, VIC, AustraliaDepartment of Medical Biology, The University of Melbourne, Parkville, VIC, AustraliaWalter and Eliza Hall Institute of Medical Research, Parkville, VIC, AustraliaDepartment of Medical Biology, The University of Melbourne, Parkville, VIC, Australiaγδ T cells play an essential role in the immune response to many pathogens, including Plasmodium. However, long-lasting effects of infection on the γδ T cell population still remain inadequately understood. This study focused on assessing molecular and functional changes that persist in the γδ T cell population following resolution of malaria infection. We investigated transcriptional changes and memory-like functional capacity of malaria pre-exposed γδ T cells using a Plasmodiumchabaudi infection model. We show that multiple genes associated with effector function (chemokines, cytokines and cytotoxicity) and antigen-presentation were upregulated in P. chabaudi-exposed γδ T cells compared to γδ T cells from naïve mice. This transcriptional profile was positively correlated with profiles observed in conventional memory CD8+ T cells and was accompanied by enhanced reactivation upon secondary encounter with Plasmodium-infected red blood cells in vitro. Collectively our data demonstrate that Plasmodium exposure result in “memory-like imprints” in the γδ T cell population and also promotes γδ T cells that can support antigen-presentation during subsequent infections.https://www.frontiersin.org/article/10.3389/fimmu.2020.582358/fullRNA-SeqmemoryPlasmodiumchabaudiγδ T cell |
| spellingShingle | Rasika Kumarasingha Rasika Kumarasingha Lisa J. Ioannidis Lisa J. Ioannidis Waruni Abeysekera Waruni Abeysekera Stephanie Studniberg Stephanie Studniberg Dinidu Wijesurendra Dinidu Wijesurendra Ramin Mazhari Ramin Mazhari Daniel P. Poole Ivo Mueller Ivo Mueller Louis Schofield Louis Schofield Louis Schofield Diana S. Hansen Diana S. Hansen Emily M. Eriksson Emily M. Eriksson Transcriptional Memory-Like Imprints and Enhanced Functional Activity in γδ T Cells Following Resolution of Malaria Infection Frontiers in Immunology RNA-Seq memory Plasmodium chabaudi γδ T cell |
| title | Transcriptional Memory-Like Imprints and Enhanced Functional Activity in γδ T Cells Following Resolution of Malaria Infection |
| title_full | Transcriptional Memory-Like Imprints and Enhanced Functional Activity in γδ T Cells Following Resolution of Malaria Infection |
| title_fullStr | Transcriptional Memory-Like Imprints and Enhanced Functional Activity in γδ T Cells Following Resolution of Malaria Infection |
| title_full_unstemmed | Transcriptional Memory-Like Imprints and Enhanced Functional Activity in γδ T Cells Following Resolution of Malaria Infection |
| title_short | Transcriptional Memory-Like Imprints and Enhanced Functional Activity in γδ T Cells Following Resolution of Malaria Infection |
| title_sort | transcriptional memory like imprints and enhanced functional activity in γδ t cells following resolution of malaria infection |
| topic | RNA-Seq memory Plasmodium chabaudi γδ T cell |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2020.582358/full |
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