Insulin Preferentially Regulates the Activity of Parasympathetic Preganglionic Neurons over Sympathetic Preganglionic Neurons
Background Insulin is a peptide hormone that regulates post-prandial physiology, and it is well known that insulin controls homeostasis at least in part via the central nervous system. In particular, insulin alters the activity of neurons within the autonomic nervous system. However, currently avail...
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| Format: | Article |
| Language: | English |
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Korean Endocrine Society
2023-10-01
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| Series: | Endocrinology and Metabolism |
| Subjects: | |
| Online Access: | http://www.e-enm.org/upload/pdf/enm-2023-1725.pdf |
| _version_ | 1797650421635874816 |
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| author | Uisu Hyun Yoon Young Kweon Jong-Woo Sohn |
| author_facet | Uisu Hyun Yoon Young Kweon Jong-Woo Sohn |
| author_sort | Uisu Hyun |
| collection | DOAJ |
| description | Background Insulin is a peptide hormone that regulates post-prandial physiology, and it is well known that insulin controls homeostasis at least in part via the central nervous system. In particular, insulin alters the activity of neurons within the autonomic nervous system. However, currently available data are mostly from unidentified brainstem neurons of the dorsal motor nucleus of the vagus nerve (DMV). Methods In this study, we used several genetically engineered mouse models to label distinct populations of neurons within the brainstem and the spinal cord for whole-cell patch clamp recordings and to assess several in vivo metabolic functions. Results We first confirmed that insulin directly inhibited cholinergic (parasympathetic preganglionic) neurons in the DMV. We also found inhibitory effects of insulin on both the excitatory and inhibitory postsynaptic currents recorded in DMV cholinergic neurons. In addition, GABAergic neurons of the DMV and nucleus tractus solitarius were inhibited by insulin. However, insulin had no effects on the cholinergic sympathetic preganglionic neurons of the spinal cord. Finally, we obtained results suggesting that the insulin-induced inhibition of parasympathetic preganglionic neurons may not play a critical role in the regulation of glucose homeostasis and gastrointestinal motility. Conclusion Our results demonstrate that insulin inhibits parasympathetic neuronal circuitry in the brainstem, while not affecting sympathetic neuronal activity in the spinal cord. |
| first_indexed | 2024-03-11T16:00:01Z |
| format | Article |
| id | doaj.art-946e641039264ea383a2e57995328b74 |
| institution | Directory Open Access Journal |
| issn | 2093-596X 2093-5978 |
| language | English |
| last_indexed | 2024-03-11T16:00:01Z |
| publishDate | 2023-10-01 |
| publisher | Korean Endocrine Society |
| record_format | Article |
| series | Endocrinology and Metabolism |
| spelling | doaj.art-946e641039264ea383a2e57995328b742023-10-25T07:28:35ZengKorean Endocrine SocietyEndocrinology and Metabolism2093-596X2093-59782023-10-0138554555610.3803/EnM.2023.17252420Insulin Preferentially Regulates the Activity of Parasympathetic Preganglionic Neurons over Sympathetic Preganglionic NeuronsUisu Hyun0Yoon Young Kweon1Jong-Woo Sohn Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, KoreaBackground Insulin is a peptide hormone that regulates post-prandial physiology, and it is well known that insulin controls homeostasis at least in part via the central nervous system. In particular, insulin alters the activity of neurons within the autonomic nervous system. However, currently available data are mostly from unidentified brainstem neurons of the dorsal motor nucleus of the vagus nerve (DMV). Methods In this study, we used several genetically engineered mouse models to label distinct populations of neurons within the brainstem and the spinal cord for whole-cell patch clamp recordings and to assess several in vivo metabolic functions. Results We first confirmed that insulin directly inhibited cholinergic (parasympathetic preganglionic) neurons in the DMV. We also found inhibitory effects of insulin on both the excitatory and inhibitory postsynaptic currents recorded in DMV cholinergic neurons. In addition, GABAergic neurons of the DMV and nucleus tractus solitarius were inhibited by insulin. However, insulin had no effects on the cholinergic sympathetic preganglionic neurons of the spinal cord. Finally, we obtained results suggesting that the insulin-induced inhibition of parasympathetic preganglionic neurons may not play a critical role in the regulation of glucose homeostasis and gastrointestinal motility. Conclusion Our results demonstrate that insulin inhibits parasympathetic neuronal circuitry in the brainstem, while not affecting sympathetic neuronal activity in the spinal cord.http://www.e-enm.org/upload/pdf/enm-2023-1725.pdfautonomic nervous systemmedulla oblongataspinal cordcholinergic neuronselectrophysiologyautonomic function |
| spellingShingle | Uisu Hyun Yoon Young Kweon Jong-Woo Sohn Insulin Preferentially Regulates the Activity of Parasympathetic Preganglionic Neurons over Sympathetic Preganglionic Neurons Endocrinology and Metabolism autonomic nervous system medulla oblongata spinal cord cholinergic neurons electrophysiology autonomic function |
| title | Insulin Preferentially Regulates the Activity of Parasympathetic Preganglionic Neurons over Sympathetic Preganglionic Neurons |
| title_full | Insulin Preferentially Regulates the Activity of Parasympathetic Preganglionic Neurons over Sympathetic Preganglionic Neurons |
| title_fullStr | Insulin Preferentially Regulates the Activity of Parasympathetic Preganglionic Neurons over Sympathetic Preganglionic Neurons |
| title_full_unstemmed | Insulin Preferentially Regulates the Activity of Parasympathetic Preganglionic Neurons over Sympathetic Preganglionic Neurons |
| title_short | Insulin Preferentially Regulates the Activity of Parasympathetic Preganglionic Neurons over Sympathetic Preganglionic Neurons |
| title_sort | insulin preferentially regulates the activity of parasympathetic preganglionic neurons over sympathetic preganglionic neurons |
| topic | autonomic nervous system medulla oblongata spinal cord cholinergic neurons electrophysiology autonomic function |
| url | http://www.e-enm.org/upload/pdf/enm-2023-1725.pdf |
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