Arbuscular mycorrhizal fungus changes alfalfa response to pathogen infection activated by pea aphid infestation
IntroductionArbuscular mycorrhizal (AM) fungi are important for the resistance of plants to insect infestation and diseases. However, the effect of AM fungal colonization of plants response to pathogen infection activated by pea aphid infestation is unknown. Pea aphid (Acyrthosiphon pisum) and the f...
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1074592/full |
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| author | Yajie Wang Yajie Wang Yajie Wang Yingde Li Yingde Li Yingde Li Tingyu Duan Tingyu Duan Tingyu Duan |
| author_facet | Yajie Wang Yajie Wang Yajie Wang Yingde Li Yingde Li Yingde Li Tingyu Duan Tingyu Duan Tingyu Duan |
| author_sort | Yajie Wang |
| collection | DOAJ |
| description | IntroductionArbuscular mycorrhizal (AM) fungi are important for the resistance of plants to insect infestation and diseases. However, the effect of AM fungal colonization of plants response to pathogen infection activated by pea aphid infestation is unknown. Pea aphid (Acyrthosiphon pisum) and the fungal pathogen Phoma medicaginis severely limit alfalfa production worldwide.MethodsThis study established an alfalfa (Medicago sativa)–AM fungus (Rhizophagus intraradices)–pea aphid–P. medicaginis experimental system to clarify the effects of an AM fungus on the host plant response to insect infestation and subsequent fungal pathogen infection.ResultsPea aphid increased the disease incidence of P. medicaginis by 24.94%. The AM fungus decreased the disease index by 22.37% and enhanced alfalfa growth by increasing the uptake of total nitrogen and total phosphorus. The aphid induced polyphenol oxidase activity of alfalfa, and the AM fungus enhanced plant-defense enzyme activity against aphid infestation and subsequent P. medicaginis infection. In addition, the AM fungus increased the contents of jasmonic acid and abscisic acid in plants exposed to aphid infestation or pathogen infection. Abscisic acid and genes associated with the gene ontology term “hormone binding” were upregulated in aphid-infested or pathogen-infected alfalfa.DiscussionThe results demonstrate that an AM fungus enhances plant defense and signaling components induced by aphid infestation, which may contribute to improved defense against subsequent pathogen infection. |
| first_indexed | 2024-04-10T16:41:13Z |
| format | Article |
| id | doaj.art-946f666a16ff4debaba0ac123ce680e6 |
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| language | English |
| last_indexed | 2024-04-10T16:41:13Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-946f666a16ff4debaba0ac123ce680e62023-02-08T06:57:17ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-02-011310.3389/fmicb.2022.10745921074592Arbuscular mycorrhizal fungus changes alfalfa response to pathogen infection activated by pea aphid infestationYajie Wang0Yajie Wang1Yajie Wang2Yingde Li3Yingde Li4Yingde Li5Tingyu Duan6Tingyu Duan7Tingyu Duan8State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Lanzhou University, Lanzhou, ChinaKey Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou, ChinaCollege of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, ChinaState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Lanzhou University, Lanzhou, ChinaKey Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou, ChinaCollege of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, ChinaState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Lanzhou University, Lanzhou, ChinaKey Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou, ChinaCollege of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, ChinaIntroductionArbuscular mycorrhizal (AM) fungi are important for the resistance of plants to insect infestation and diseases. However, the effect of AM fungal colonization of plants response to pathogen infection activated by pea aphid infestation is unknown. Pea aphid (Acyrthosiphon pisum) and the fungal pathogen Phoma medicaginis severely limit alfalfa production worldwide.MethodsThis study established an alfalfa (Medicago sativa)–AM fungus (Rhizophagus intraradices)–pea aphid–P. medicaginis experimental system to clarify the effects of an AM fungus on the host plant response to insect infestation and subsequent fungal pathogen infection.ResultsPea aphid increased the disease incidence of P. medicaginis by 24.94%. The AM fungus decreased the disease index by 22.37% and enhanced alfalfa growth by increasing the uptake of total nitrogen and total phosphorus. The aphid induced polyphenol oxidase activity of alfalfa, and the AM fungus enhanced plant-defense enzyme activity against aphid infestation and subsequent P. medicaginis infection. In addition, the AM fungus increased the contents of jasmonic acid and abscisic acid in plants exposed to aphid infestation or pathogen infection. Abscisic acid and genes associated with the gene ontology term “hormone binding” were upregulated in aphid-infested or pathogen-infected alfalfa.DiscussionThe results demonstrate that an AM fungus enhances plant defense and signaling components induced by aphid infestation, which may contribute to improved defense against subsequent pathogen infection.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1074592/fullAM fungusplant–pathogen–insect interactionMedicago sativaPhoma medicaginispea aphid |
| spellingShingle | Yajie Wang Yajie Wang Yajie Wang Yingde Li Yingde Li Yingde Li Tingyu Duan Tingyu Duan Tingyu Duan Arbuscular mycorrhizal fungus changes alfalfa response to pathogen infection activated by pea aphid infestation Frontiers in Microbiology AM fungus plant–pathogen–insect interaction Medicago sativa Phoma medicaginis pea aphid |
| title | Arbuscular mycorrhizal fungus changes alfalfa response to pathogen infection activated by pea aphid infestation |
| title_full | Arbuscular mycorrhizal fungus changes alfalfa response to pathogen infection activated by pea aphid infestation |
| title_fullStr | Arbuscular mycorrhizal fungus changes alfalfa response to pathogen infection activated by pea aphid infestation |
| title_full_unstemmed | Arbuscular mycorrhizal fungus changes alfalfa response to pathogen infection activated by pea aphid infestation |
| title_short | Arbuscular mycorrhizal fungus changes alfalfa response to pathogen infection activated by pea aphid infestation |
| title_sort | arbuscular mycorrhizal fungus changes alfalfa response to pathogen infection activated by pea aphid infestation |
| topic | AM fungus plant–pathogen–insect interaction Medicago sativa Phoma medicaginis pea aphid |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1074592/full |
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