| Summary: | <i>Lactobacillus plantarum</i> is a catalase-negative species and distributes in human intestinal tracts. However, the cytoprotective effects of the catalase-activated <i>L. plantarum</i> strain have yet to be exploited against reactive oxygen species (ROS). Here, a catalase-activated <i>L. plantarum</i> CGMCC 6888 (CatA<sup>+</sup>) was obtained using exogenous added heme. The scavenging free radical abilities of this strain were obviously increased. Moreover, the activated catalase A in <i>L. plantarum</i> CGMCC 6888 endowed the intestinal epithelium NCM460 with lower ROS content after degrading H<sub>2</sub>O<sub>2</sub>. In addition, the transcription levels of Nrf2 and Nrf2-related antioxidant enzyme genes (HO-1, GCLC, NQO-1 and TXNRD1) and tight junction protein genes (ZO-1, OCLN, and JAM-1) were upregulated significantly when co-incubated with CGMCC 6888/CatA<sup>+</sup>. This work confirmed that the catalase A conferred <i>L. plantarum</i> with the strong protection effects in the intestinal epithelial cells against ROS.
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