The Novel Monoclonal IgG<sub>1</sub>-Antibody AB90-E8 as a Diagnostic Tool to Rapidly Distinguish <i>Aspergillus fumigatus</i> from Other Human Pathogenic <i>Aspergillus</i> Species
In most cases, invasive aspergillosis (IA) is caused by <i>A. fumigatus</i>, though infections with other <i>Aspergillus</i> spp. with lower susceptibilities to amphotericin B (AmB) gain ground. <i>A. terreus</i>, for instance, is the second leading cause of IA in...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/6/622 |
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| author | Tamara Katharina Kakoschke Christoph Kleinemeier Thomas Knösel Sara Carina Kakoschke Frank Ebel |
| author_facet | Tamara Katharina Kakoschke Christoph Kleinemeier Thomas Knösel Sara Carina Kakoschke Frank Ebel |
| author_sort | Tamara Katharina Kakoschke |
| collection | DOAJ |
| description | In most cases, invasive aspergillosis (IA) is caused by <i>A. fumigatus</i>, though infections with other <i>Aspergillus</i> spp. with lower susceptibilities to amphotericin B (AmB) gain ground. <i>A. terreus</i>, for instance, is the second leading cause of IA in humans and of serious concern because of its high propensity to disseminate and its in vitro and in vivo resistance to AmB. An early differentiation between <i>A. fumigatus</i> and <i>non-A. fumigatus</i> infections could swiftly recognize a potentially ineffective treatment with AmB and lead to the lifesaving change to a more appropriate drug regime in high-risk patients. In this study, we present the characteristics of the monoclonal IgG<sub>1</sub>-antibody AB90-E8 that specifically recognizes a surface antigen of <i>A. fumigatus</i> and the closely related, but not human pathogenic <i>A. fischeri</i>. We show immunostainings on fresh frozen sections as well as on incipient mycelium picked from agar plates with tweezers or by using the expeditious tape mount technique. All three methods have a time advantage over the common procedures currently used in the routine diagnosis of IA and outline the potential of AB90-E8 as a rapid diagnostic tool. |
| first_indexed | 2024-03-11T02:16:32Z |
| format | Article |
| id | doaj.art-94761cf87f5c4dc293e2025cc6a4dc04 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-11T02:16:32Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-94761cf87f5c4dc293e2025cc6a4dc042023-11-18T11:08:33ZengMDPI AGJournal of Fungi2309-608X2023-05-019662210.3390/jof9060622The Novel Monoclonal IgG<sub>1</sub>-Antibody AB90-E8 as a Diagnostic Tool to Rapidly Distinguish <i>Aspergillus fumigatus</i> from Other Human Pathogenic <i>Aspergillus</i> SpeciesTamara Katharina Kakoschke0Christoph Kleinemeier1Thomas Knösel2Sara Carina Kakoschke3Frank Ebel4Department of Oral and Maxillofacial Surgery and Facial Plastic Surgery, University Hospital, Ludwig-Maximilians-University Munich, Lindwurmstrasse 2a, 80337 Munich, GermanyInstitute for Infectious Diseases and Zoonoses, Ludwig-Maximilians-University Munich, 85764 Oberschleissheim, GermanyInstitute of Pathology, Ludwig-Maximilians-University Munich, 81377 Munich, GermanyDepartment of General, Visceral and Transplant Surgery, University Hospital, Ludwig-Maximilians-University Munich, Marchioninistrasse 15, 81337 Munich, GermanyInstitute for Infectious Diseases and Zoonoses, Ludwig-Maximilians-University Munich, 85764 Oberschleissheim, GermanyIn most cases, invasive aspergillosis (IA) is caused by <i>A. fumigatus</i>, though infections with other <i>Aspergillus</i> spp. with lower susceptibilities to amphotericin B (AmB) gain ground. <i>A. terreus</i>, for instance, is the second leading cause of IA in humans and of serious concern because of its high propensity to disseminate and its in vitro and in vivo resistance to AmB. An early differentiation between <i>A. fumigatus</i> and <i>non-A. fumigatus</i> infections could swiftly recognize a potentially ineffective treatment with AmB and lead to the lifesaving change to a more appropriate drug regime in high-risk patients. In this study, we present the characteristics of the monoclonal IgG<sub>1</sub>-antibody AB90-E8 that specifically recognizes a surface antigen of <i>A. fumigatus</i> and the closely related, but not human pathogenic <i>A. fischeri</i>. We show immunostainings on fresh frozen sections as well as on incipient mycelium picked from agar plates with tweezers or by using the expeditious tape mount technique. All three methods have a time advantage over the common procedures currently used in the routine diagnosis of IA and outline the potential of AB90-E8 as a rapid diagnostic tool.https://www.mdpi.com/2309-608X/9/6/622invasive aspergillosis<i>Aspergillus fumigatus</i>monoclonal antibodyfungal diagnostics |
| spellingShingle | Tamara Katharina Kakoschke Christoph Kleinemeier Thomas Knösel Sara Carina Kakoschke Frank Ebel The Novel Monoclonal IgG<sub>1</sub>-Antibody AB90-E8 as a Diagnostic Tool to Rapidly Distinguish <i>Aspergillus fumigatus</i> from Other Human Pathogenic <i>Aspergillus</i> Species Journal of Fungi invasive aspergillosis <i>Aspergillus fumigatus</i> monoclonal antibody fungal diagnostics |
| title | The Novel Monoclonal IgG<sub>1</sub>-Antibody AB90-E8 as a Diagnostic Tool to Rapidly Distinguish <i>Aspergillus fumigatus</i> from Other Human Pathogenic <i>Aspergillus</i> Species |
| title_full | The Novel Monoclonal IgG<sub>1</sub>-Antibody AB90-E8 as a Diagnostic Tool to Rapidly Distinguish <i>Aspergillus fumigatus</i> from Other Human Pathogenic <i>Aspergillus</i> Species |
| title_fullStr | The Novel Monoclonal IgG<sub>1</sub>-Antibody AB90-E8 as a Diagnostic Tool to Rapidly Distinguish <i>Aspergillus fumigatus</i> from Other Human Pathogenic <i>Aspergillus</i> Species |
| title_full_unstemmed | The Novel Monoclonal IgG<sub>1</sub>-Antibody AB90-E8 as a Diagnostic Tool to Rapidly Distinguish <i>Aspergillus fumigatus</i> from Other Human Pathogenic <i>Aspergillus</i> Species |
| title_short | The Novel Monoclonal IgG<sub>1</sub>-Antibody AB90-E8 as a Diagnostic Tool to Rapidly Distinguish <i>Aspergillus fumigatus</i> from Other Human Pathogenic <i>Aspergillus</i> Species |
| title_sort | novel monoclonal igg sub 1 sub antibody ab90 e8 as a diagnostic tool to rapidly distinguish i aspergillus fumigatus i from other human pathogenic i aspergillus i species |
| topic | invasive aspergillosis <i>Aspergillus fumigatus</i> monoclonal antibody fungal diagnostics |
| url | https://www.mdpi.com/2309-608X/9/6/622 |
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